Physiology and Pharmacology
Physiol Pharmacol
Medical Sciences
http://ppj.phypha.ir
32
journal32
24765236
24765244
10.61186/phypha
(previous ISSN: 17350581)
en
jalali
1390
7
1
gregorian
2011
10
1
15
3
online
1
fulltext
en
The effect of long term treatment of lowest effective dose of para-nonylphenol on viability, morphology and proliferation of rat bone marrow mesenchymal stem cells
Cell, Stem Cell and Cancer
Cell, Stem Cell and Cancer
Experimental research article
Experimental research article
Introduction: In this study, the effect of para-nonylphenol as an environmental pollutant on viability, morphology
and proliferation of bone marrow mesenchymal stem cells was investigated.
Methods: Bone marrow mesenchymal stem cells of rat were treated with the 0.5, 1, 2.5, 3.5 and 5 μM of paranonylphenol
for a period of 21 days, then the viability of the cells were estimated using trypan blue and MTT methods.
After choosing the effective dose, the integration of the DNA was investigated using comet assay and agarose gel
electrophoresis. Mechanisms of cell death were also investigated by TUNEL assay and presence of caspase activity.
Results: The results showed that para-nonylphenol caused significant dose dependent reduction of viability and
proliferation of the cells. Comet assay, agarose gel electrophoresis and TUNEL test showed that the DNA of the cells
were damaged and broken after treatment with 0.5 and 2.5 μM of para-nonylphenol. In addition, activated caspase-3
was observed in the cytoplasm of treated cells.
Conclusion: This study showed that a very low concentration of para-nonylphenol has drastic effects on bone
marrow mesenchymal stem cells. This chemical is used in formulation of cosmetics and detergents and therfore may
have detrimental effects on the viability and proliferation of stem cells.
apoptosis, caspase, mesenchymal stem cells, para-nonylphenol, TUNEL
308
317
http://ppj.phypha.ir/browse.php?a_code=A-10-358-1&slc_lang=en&sid=1
Mohammad Husein
Abnosi
abnosi2002@yahoo.com
3200319475328460010031
3200319475328460010031
Yes
Arak University
Malek
Soleimani Mehranjani
M-Soleimani@araku.ac.ir
3200319475328460010032
3200319475328460010032
No
Arak University
Sayed Mohammad Ali
Shariatzadeh
s-shariatzadeh@araku.ac.ir
3200319475328460010033
3200319475328460010033
No
Arak University
Majid
Mahdiyeh Najafabadi
m-mahdiyeh@araku.ac.ir
3200319475328460010034
3200319475328460010034
No
Arak University
Laila
Dehdehi
M-abnsoi@araku.ac.ir
3200319475328460010035
3200319475328460010035
No
student