Physiology and Pharmacology
Physiol Pharmacol
Medical Sciences
http://ppj.phypha.ir
32
journal32
24765236
24765244
10.61186/phypha
(previous ISSN: 17350581)
en
jalali
1391
1
1
gregorian
2012
4
1
16
1
online
1
fulltext
en
Effect of ICV injection of ghrelin and leptin on T3 and T4 plasma levels in Rat
Endocrine Physiology/Pharmacology
Endocrine Physiology/Pharmacology
Experimental research article
Experimental research article
Introduction: Ghrelin, increases food intake, decreases T3 and T4 concentrations and stimulates insulin release,
while leptin reduces food intake and suppresses appetite. Considering the importance of thyroid hormones, ghrelin and
leptin in body metabolism, the purpose of this study was to investigate the effect of interactions between ghrelin and
leptin (injected via ICV route) and plasma T3 and T4 concentrations in rats.
Methods: Fifty-six Wistar male rats (230 to 250 g/BW) were randomly divided into 7 groups. Groups 1, 2 and 3
received 1, 3 and 5 nmol ghrelin, while groups 4, 5 and 6 received 1, 5, 10 nmol leptin (2 μl via lateral cerebral
ventricle). Animals in the 7th group received 5 nmol ghrelin with 10 nmol leptin via the same route for 3 days. Blood
samples were collected daily starting from one day before until one day after infusions and plasma samples were used to
assess T3 and T4 concentrations by RIA. To ensure proper cannulation, perfusion and brain slices were performed.
Results: The results indicated that ghrelin significantly decreased T3 and T4 concentrations, whereas leptin increased
these concentrations. The simultaneous injection of ghrelin and leptin significantly reduced the inhibitory effect of
ghrelin on thyroid hormones concentrations.
Conclusion: According to the results and other researchers’ suggestions, the opposite effects of leptin and ghrelin
could be emphasized and some hormonal mechanisms for these 2 hormones (transmitters) such as HPA and/or HPT
axes or MSH systems could be suggested.
Ghrelin, leptin, T3, T4, ICV injection
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http://ppj.phypha.ir/browse.php?a_code=A-10-546-1&slc_lang=en&sid=1
Ommolbanin
Amoo-Rajabi
rbani1359@gmail.com
3200319475328460010636
3200319475328460010636
Yes
Ali
Moghimi
moghimi@um.ac.ir
3200319475328460010637
3200319475328460010637
No
Homayoon
Khazali
Hkhazali@hotmail.com
3200319475328460010638
3200319475328460010638
No