en jalali 1404 3 1 gregorian 2025 6 1 29 2 online 1 fulltext

en Introduction: Genotoxicity is one of the safety concerns that can lead to damage at the genetic level. The observation of DLBS1033 using AMES and micronucleus tests was conducted to ensure the safety precautions. Methods: Ames test was done using 3 strains of Salmonella typhimurium (TA98, TA100, and TA1535) with or without metabolic activation (S9 enzyme). In vitro micronucleus test was performed with prolonged exposure without S9. The variation of DLBS1033 concentrations were 100, 600, and 1200 µg/ml. Results: The results of the Ames test (with/without S9) and in vitro micronucleus (prolonged exposure without S9) showed that DLBS1033 did not have a mutagenic effect. Conclusion: DLBS1033 was safe and did not show any genotoxic activity. Ames test, Genotoxicity, Lumbricus rubellus, Micronucleus test 106 110 http://ppj.phypha.ir/browse.php?a_code=A-10-2284-1&slc_lang=en&sid=1 2023/12/18 1402/9/27 2024/09/14 1403/6/24 Agung Heru Karsono Dexa Laboratories of Biomolecular Sciences, PT Dexa Medica, Industri Selatan V Block PP No. 7, Jababeka Industrial Estate II, Cikarang, West Java, 17550, Indonesia agung.karsono@dexa-medica.com 00319475328460037721 00319475328460037721 No Lidwina Tri Kristanti Setiawan Dexa Laboratories of Biomolecular Sciences, PT Dexa Medica, Industri Selatan V Block PP No. 7, Jababeka Industrial Estate II, Cikarang, West Java, 17550, Indonesia lidwina.setiawan@dexa-medica.com 00319475328460037722 00319475328460037722 No Raymond Rubianto Tjandrawinata Center for Pharmaceutical and Nutraceutical Research and Policy, Atma Jaya Catholic University of Indonesia, Jakarta Selatan 12930, DKI Jakarta, Indonesia raymond@dexa-medica.com 00319475328460037723 00319475328460037723 Yes

en Introduction: This study aimed to evaluate the efficacy and safety concerns of remdesivir and type 1 interferons on the clinical outcomes of hospitalized multiple sclerosis patients with COVID-19. Methods: Using electronic health records systems; this is a cross-sectional study of two years of hospital admissions in terms of COVID-19 in Iran from March 2019 to August 2021. The severities of COVID-19 outcomes were ICU admission, hospitalization days, and 30-day survival rates. Results: A total of 993 hospitalized multiple sclerosis patients with a confirmed diagnosis of COVID-19 based on PCR testing were recorded in the electronic health systems. Nearly half of these patients (50.3%) had received treatment with an anti-CD20 agent (rituximab or ocrelizumab) at the hospital admission. This group exhibited higher mortality rates, increased need for ICU admission, and longer hospitalization (p<0.05). There was a significant association between taking interferon-β1 alone (adjusted IRR=1.21, 95% CI 1.32 to 1.42) or in combination with remdesivir (adjusted IRR=1.30, 95% CI 1.18 to 1.5042) and longer hospitalization. There were no significant associations between antiviral treatment (remdesivir alone, interferon-β1- β1 alone, and interferon-β1plus remdesivir) and ICU admission (p>0.2), the in-hospital mortality rate (p>0.2), or 30-day survival rate (p>0.2). The results were similar in patients who did or did not receive anti-CD20 agents. These results were consistent among patients regardless of whether they received anti-CD20 agents. Conclusion: Our data suggest that remdesivir, interferon-β1, or a combination of both does not benefit hospitalized MS patients with COVID-19. Multiple sclerosis ,COVID-19 ,Remdesivir ,Interferon ,Anti-CD20 agents 111 122 http://ppj.phypha.ir/browse.php?a_code=A-10-2472-1&slc_lang=en&sid=1 2023/12/182024/09/29 1403/7/8 2024/09/142024/12/8 1403/9/18 Zhila Maghbooli Neuroscience Institute, Multiple Sclerosis Research Center, Tehran University of Medical Sciences, Tehran, Iran zhilayas@gmail.com 00319475328460037724 00319475328460037724 Yes Amir Kasaeian Liver and Pancreatobiliary Diseases Research Center, Digestive Diseases Research Institute, Tehran University of Medical Sciences, Tehran, Iran amir_kasaeian@yahoo.com 00319475328460037725 00319475328460037725 No Mohammad Reza Fattahi Neuroscience Institute, Multiple Sclerosis Research Center, Tehran University of Medical Sciences, Tehran, Iran mrfatahi93@gmail.com 00319475328460037726 00319475328460037726 No Tarlan Varzandi Neuroscience Institute, Multiple Sclerosis Research Center, Tehran University of Medical Sciences, Tehran, IRAN t.varzandi@gmail.com 00319475328460037727 00319475328460037727 No Sara Hamtaeigashi Neuroscience Institute, Multiple Sclerosis Research Center, Tehran University of Medical Sciences, Tehran, IRAN Sarah.Hamtaee@yahoo.com 00319475328460037728 00319475328460037728 No Sara Mohammadnabi Neuroscience Institute, Multiple Sclerosis Research Center, Tehran University of Medical Sciences, Tehran, IRAN semagh62@gmail.com 00319475328460037729 00319475328460037729 No Mohammad Ali Sahraian Neuroscience Institute, Multiple Sclerosis Research Center, Tehran University of Medical Sciences, Tehran, IRAN ms.genelab@gmail.com 00319475328460037730 00319475328460037730 No

en Introduction: Histamine directly interacts with airways smooth muscles to initiate contraction. This occurs even at low concentrations among individuals predisposed to increased airway inflammation and asthma. Methods: A digital vitalograph, nebulizer, histamine, and pulse oximeter were used in this research. A total of one hundred and sixty subjects were selected and categorized into two groups; Group 1 (Test subject) and Group 2 (Control), each group contained eighty (80) subjects. Group 1 comprised occupationally exposed vulcanizers and Group 2 comprised selected students from the University of Calabar as control. Both groups comprised young male subjects of equal age (20-40 years), devoid of pre-existing respiratory disorders or on any medication(s). Demographic parameters were used to obtain sufficient information for overall analysis. Lung parameters (FVC, FEV1, PEFR, and FEV1%) were evaluated using a Vitalograph Spirometer. Results: Basal measurement showed that Group 1 had a moderate decrease in lung volumes (FVC, FEV1, PEFR, and FEV1%) of 65 subjects (representing 81.25%) and a mild decrease in 15 subjects (18.75%); all the subjects in Group 2 showed a mild decrease in lung volumes. Inhalation of histamine mist of different concentrations showed that Group 1 had a severe decrease in lung volumes of 70 subjects (87.5%) and a mild decrease in 10 subjects (12.5%). All Subjects in Group 2 showed a mild decrease in lung volumes. Conclusion: Histamine provocative test helps to evaluate asthma signs and symptoms. Airways Hyper reactiv-ity is associated with inflammation as a consequence of airway hyperreflexia. It is evidenced that associated pollutants and their chronic inhalation by the vulcanizers have a retrogressive impact on their airways which manifests with hyper response an indication for asthma signs. Spirometric evaluation,Vulcanizer,Histamine,Bronchial responses 123 134 http://ppj.phypha.ir/browse.php?a_code=A-10-2241-1&slc_lang=en&sid=1 2023/12/182024/09/292023/10/28 1402/8/6 2024/09/142024/12/82024/10/5 1403/7/14 Utionkpan Linna Paul Human Physiology, University of Calabar, Calabar, Nigeria linns.paul@yahoo.com 00319475328460038098 0000000319460675 Yes Helyn A Ikem Human Physiology, University of Calabar, Calabar, Nigeria 00319475328460038099 00319475328460038099 No Cecilia Peter Human Physiology, University of Calabar, Calabar, Nigeria 00319475328460038100 00319475328460038100 No Solomon Beshel Zoology and Environmental Biology, University of Calabar, Calabar, Nigeria 00319475328460038101 00319475328460038101 No

en Introduction: One of the most common cancers of the oral cavity is oral squamous cell carcinoma (OSCC). Previous studies have provided evidence that apolipoprotein C-I (Apo C-I) has an oncogenic role beyond its classical function. Although it has been investigated in prostate, breast, and lung cancer, there is no information available regarding Apo C-I expression in tumor and pri-tumor tissues of OSCC patients. Thus, the goal of the present study was to unravel the expression of Apo C-I in OSCC and investigate its correlation with the survival and grade of OSCC patients. Methods: The Apo C-I mRNA level was measured in tumor and pri-tumor tissues of 16 OSCC patients. In addition, the 34 paraffin-embedded tissues of OSCC patients and IHC technique were used to analyze the association of Apo C-I protein with the survival of OSCC patients.  Results: The mRNA (P=0.0386) gene expression of Apo C-I showed a statistically significant difference between tumor and pri-tumor tissues of OSCC patients. It seems that a high protein level of Apo C-I is related with poor survival of OSCC patients (P=0.04). The Apo C-I protein was positively correlated with the tumor site (Pearson r= 0.485, P=0.0036) but not the grade (Pearson r= 0.2295, P=0.1917). Our data showed that high Apo C-I protein levels might be correlated with poor survival of OSCC patients. Conclusion: Our findings suggest that patients with high protein levels of Apo C-I may have lower survival, making it a potential prognostic factor for OSCC. However, further investigation is necessary to establish this concept. Apolipoprotein C-I, Oral Squamous Cell Carcinoma, Survival 135 145 http://ppj.phypha.ir/browse.php?a_code=A-10-1718-1&slc_lang=en&sid=1 2023/12/182024/09/292023/10/282024/07/1 1403/4/11 2024/09/142024/12/82024/10/52024/09/25 1403/7/4 Kurosh Hamzanloui Moghadam Department of Clinical Biochemistry, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran kuroshmqdm75@gmail.com 00319475328460037735 00319475328460037735 No Walla Alazzawi Department of Clinical Biochemistry, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran hussain89abd@gmail.com 00319475328460037736 00319475328460037736 No Samira Derakhshan Oral and Maxillofacial Pathology Department, School of Dentistry, Tehran University of Medical Sciences, Tehran, Iran samderakhshan.den@gmail.com 00319475328460037737 00319475328460037737 No Abbas Karimi Oral and Maxillofacial Surgery Department, School of Dentistry, Tehran University of Medical Sciences, Tehran, Iran abkarimi@tums.ac.ir 00319475328460037738 00319475328460037738 No Zahra Shahsavari Department of Clinical Biochemistry, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran shahsavari.z@gmail.com 00319475328460037739 00319475328460037739 No Afsaneh Goudarzi Department of Clinical Biochemistry, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran afsaneh.goudarzi@hotmail.com 00319475328460037740 00319475328460037740 Yes

en Introduction: Methamphetamine (MA) addiction is a major global public health concern, yet there is currently no approved medication that effectively treats this addiction. Kisspeptin is a neuropeptide that has a role in the reproductive system, metabolism and energy balance, and metastasis suppression in different types of cancers. The kisspeptin receptors, GPR54 are widely distributed in the brain’s memory-related structures. Previous studies have revealed that the opioid system contributes to the addictive effects of MA. Additionally, preclinical studies have shown that a derivative of kisspeptin possesses anti-opioid properties. This study aimed to clarify the role of kisspeptin-13 (KP-13) on reward and reinstatement-related memory associated with MA in the conditioned place preference test. Methods: We evaluated pre-treatment with intracerebroventricular KP-13 for 3 consecutive days (2.5 μl/nostril, once a day) in a conditioned place preference test induced by MA. MA was administered intraperitoneally at a dose ranging from 1 to 7 mg/kg, once daily, beginning at 1 mg/kg on day one and increasing by 1 mg/kg per day up to the day 7. Results: We found that KP-13 suppresses the reward behavior in MA-treated rats, while it has no significant effect on reinstatement behavior after one week of MA cessation, which could be attributed to the ineffective dose of KP-13. Conclusion: The findings indicate that KP-13 alters the rewarding and motivating effects of MA. Further research involving both multiple and single administrations of KP-13 before the reinstatement test is necessary to throw light on its impact on withdrawal-related reinstatement. Kisspeptin-13, Methamphetamine, Reward, Reinstatement, Condition place preference 146 154 http://ppj.phypha.ir/browse.php?a_code=A-10-2237-2&slc_lang=en&sid=1 2023/12/182024/09/292023/10/282024/07/12024/01/16 1402/10/26 2024/09/142024/12/82024/10/52024/09/252024/09/7 1403/6/17 Somayeh Nazari Student Research Committee, School of Advanced Technologies in Medicine, Mazandaran University of Medical Sciences, Sari, Iran snazari70@yahoo.com 00319475328460037741 00319475328460037741 No Mobina Gheibi Student Research Committee, Mazandaran University of Medical Sciences, Sari, Iran mobinagheibi78@gmail.com 00319475328460037742 00319475328460037742 No Hamed Ghazvini Department of Neuroscience, School of Advanced Technologies in Medicine, Mazandaran University of Medical Sciences, Sari, Iran hghazvini1@gmail.com 00319475328460037743 00319475328460037743 No Raheleh Rafaiee Department of Neuroscience, School of Advanced Technologies in Medicine, Mazandaran University of Medical Sciences, Sari, Iran rachel.rafaie@yahoo.com 00319475328460037744 00319475328460037744 No seyedeh masoumeh Seyedhosseini Tamijani Department of Neuroscience, School of Advanced Technologies in Medicine, Mazandaran University of Medical Sciences, Sari, Iran m.seyedhoseini@mazums.ac.ir 00319475328460037745 00319475328460037745 Yes

en Introduction: Endometriosis (EMS) is a highly prevalent gynecological disorder with substantial health consequences, affecting as many as 10% of women in their reproductive years. Although EMS is widespread, its intricate origin and pathophysiology are not well understood. This study investigated the molecular characteristics of EMS by examining the expression patterns of long noncoding RNAs (lncRNAs), microRNAs (miRNAs), and genes linked to the condition. Methods: The study used publicly accessible datasets, namely RNA-seq data for long non-coding RNAs (GSE105764), miRNA expression data (GSE105765), and gene expression data (GSE12768), to compare samples of ectopic and eutopic endometrial samples. The study conducted differential expression analysis, pathway enrichment analyses, protein-protein interaction network creation, and hub gene identification to uncover the molecular markers linked to EMS.  Results: The investigation identified substantial dysregulation of lncRNAs, miRNAs, and genes in endometriotic tissues compared with the control eutopic endometrium. Hub genes, including CDH1, ESR1, GATA4, PGR, FOXA2, EPCAM, APOA1, BDNF, FGFR2, and PAX2, dominate the molecular landscape. miR-6500-3p has been identified as a pivotal regulator that influences the expression of seven essential genes. Pathway enrichment analysis identified biological processes, including embryonic organ morphogenesis, immunological response, and medication metabolism. Exploring lncRNA-mediated regulatory axes, particularly H19 and MIR600HG, suggests complex networks that may clarify the molecular underpinnings of EMS. Conclusion: This thorough investigation offers significant insight into the molecular pathways underlying EMS. Hub genes and regulatory miRNAs, including miR-6500-3p, have been identified as prospective targets for therapeutic interventions. Pathway enrichment studies provide a more comprehensive understanding of the biological processes implicated in EMS, indicating new possibilities for therapeutic interventions. Endometriosis, lncRNAs, miRNAs, Hub Genes, Bioinformatics 155 170 http://ppj.phypha.ir/browse.php?a_code=A-10-2375-1&slc_lang=en&sid=1 2023/12/182024/09/292023/10/282024/07/12024/01/162024/04/27 1403/2/8 2024/09/142024/12/82024/10/52024/09/252024/09/72024/08/31 1403/6/10 Shahrzad Zhaeentan Department of Anatomy, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran zhaeentan.shahrzad@yahoo.com 00319475328460037746 00319475328460037746 No Fardin Amidi Department of Anatomy, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran amidifardin@yahoo.com 00319475328460037747 00319475328460037747 No Ashraf Moini Department of Gynecology and Obstetrics, Arash Women’s Hospital, Tehran University of Medical Sciences, Tehran, Iran moeinias@tums.ac.ir 00319475328460037748 00319475328460037748 No Seyed Danial Mohammadi Department of Anatomy, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran drsdmohammadi@gmail.com 00319475328460037749 00319475328460037749 No Aligholi Sobhani Department of Anatomy, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran sobhania@tums.ac.ir 00319475328460037750 00319475328460037750 No Masoomeh Nataj Majd Department of Infertility, Arash Women’s Hospital, Tehran University of Medical Sciences, Tehran, Iran mnataj54@yahoo.com 00319475328460037751 00319475328460037751 No Mahshad khodarahmian Department of Anatomy, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran ma.khodarahmian@gmail.com 00319475328460037752 00319475328460037752 Yes

en Introduction: Caffeine, as a popular drink, along with exercise training, may help restore altered gene expres-sion in high-fat diet (HFD)-induced obesity. This study examined the effects of exercise training, caffeine consumption, and their interaction on inflammation and genes involved in metabolism in rats fed an HFD. Methods: Eighty male Wistar rats were separated into two groups: HFD and normal diet (ND). Each group was subsequently divided into four groups: sedentary, caffeine-only, exercise, and caffeine-plus-exercise. For eight weeks, the animals in the training groups engaged in aerobic exercise on a mo-torized treadmill for 60 minutes, five times per week. Animals in the caffeine group ingested a solution containing caffeine daily (6 mg/kg/bw). The expression of Peroxisome proliferator-acti­vated receptor-gamma coactivator 1 alpha (PGC-1α) and fibronectin type III domain-containing (FNDC5) genes in the calf muscle, uncoupling protein-1 (UCP1) in subcutaneous adipose tissue, NF-KB and TLR4 in vis-ceral adipose tissue, and fetuin-A (Fet-A) in the liver were investigated. Results: The findings demonstrated that HFD significantly elevated the NF-Kβ gene and downregulated the skeletal muscle PGC-1α and FNDC5 genes, as well as serum fetuin-A. UCP-1 (366% vs. 56%), FNDC5 (26% vs. 54%), and PGC-1α (40% vs. 1700%) genes were all considerably elevated by exercise training and caffeine supplementation, respectively. Additionally, exercise training reduced TLR4 and NF-Kβ expression in visceral adipose tissue and liver Fet-A gene expression. Furthermore, following HFD, when compared to the sedentary group, exercise training with and without caffeine consumption decreased the NF-Kβ gene and liver Fet-A and increased PGC1-α, FNDC5, UCP1, and serum Fet-A. Conclusion: These findings support the idea that exercise and caffeine may reduce inflammation by downregulating genes involved in inflammation and adipose tissue browning. Exercise training, Caffeine, Supplementation, Brown Adipose Tissue Uncoupling Protein, Inflammation 171 183 http://ppj.phypha.ir/browse.php?a_code=A-10-2373-1&slc_lang=en&sid=1 2023/12/182024/09/292023/10/282024/07/12024/01/162024/04/272024/04/26 1403/2/7 2024/09/142024/12/82024/10/52024/09/252024/09/72024/08/312024/09/25 1403/7/4 Mohammad Rahman Rahimi Department of Exercise Physiology, University of Kurdistan, Sanandaj, Iran r.rahimi@uok.ac.ir 00319475328460037753 00319475328460037753 Yes Sanaz Mahmazi Department of Genetics, Faculty of Basic Sciences, Islamic Azad University, Zanjan Branch, Zanjan, Iran sanazmahmazi@yahoo.com 00319475328460037754 00319475328460037754 No Mehdi Abbaspoor Department of Physical Education and Sport Sciences, Shahid Bahonar University of Kerman, Kerman, Iran m.abbaspoor@uk.ac.ir 00319475328460037755 00319475328460037755 No Mehdi Rahnema Department of Genetics, Faculty of Basic Sciences, Islamic Azad University, Zanjan Branch, Zanjan, Iran meh_rahnema@yahoo.com 00319475328460037756 00319475328460037756 No Dara Latif Saifalddin Department of Physical Education and Sport Sciences, University of Halabja, Kurdistan region, Iraq dara.saifaddin@uoh.edu.iq 00319475328460037757 00319475328460037757 No Nasser Ghanbari Department of Exercise Physiology, University of Kurdistan, Sanandaj, Iran naser2617@gmail.com 00319475328460037758 00319475328460037758 No Sarkawt Kolahdouzi Department of Exercise Physiology, Faculty of Sport Sciences, University of Mazandaran, Babolsar, Iran skolahdouzi@yahoo.com 00319475328460037759 00319475328460037759 No Hadi Golpasandi Department of Exercise Physiology, University of Kurdistan, Sanandaj, Iran hadi.golpasandi@uok.ac.ir 00319475328460037760 00319475328460037760 No

en Introduction: Ovarian torsion is an emergency condition that occurs when the adnexa undergoes complete or partial rotation. Following ovarian torsion, damage caused by ischemia/reperfusion can impact fertility and sex hormone secretion. Our study aims to investigate the role of safranal in preventing ovarian ischemia-reperfusion injury in rats. Methods: Animal subgroups included: sham, ovarian torsion/detorsion (OT), ovarian torsion/detorsion with the treatment of safranal (OTS 0.1, 0.5), and safranal group (OS 0.1, 0.5). Ovarian torsion was induced in the left ovary of rats for 2 hours. Intraperitoneal treatment with safranal at 0.1 and 0.5 mg/kg doses was performed 30 minutes before the detorsion operation. 48 h after detorsion, ovarian tissue was collected to evaluate the histopathological scores and apoptosis gene expression of Bcl-2, caspase 3, and Bax. Blood samples were collected to measure plasma estradiol levels and oxidative stress parameters. Results: Analyses demonstrated that ovarian follicular damage was accompanied by increased serum malondialdehyde (MDA) levels and increased expression of Bcl2 and caspase-3 genes. Additionally, serum levels of glutathione peroxidase (GPX), estrogen, and superoxide dismutase (SOD) decreased in the OT group. However, treatment with safranal 0.5 mg/kg was accompanied by an improvement in the histopathological score of the ovarian tissue and a reduction in apoptosis and oxidative stress. Conclusion: Safranal exhibits antioxidant properties in a rat model of ovarian torsion and could be considered a cost-effective therapeutic option for ovarian detorsion in gynecological clinics.  Safranal,Oxidative Stress,Ischemia-reperfusion,Ovaries 184 193 http://ppj.phypha.ir/browse.php?a_code=A-10-2328-1&slc_lang=en&sid=1 2023/12/182024/09/292023/10/282024/07/12024/01/162024/04/272024/04/262024/02/19 1402/11/30 2024/09/142024/12/82024/10/52024/09/252024/09/72024/08/312024/09/252024/10/5 1403/7/14 Marzieh Ebrahimi Student Research Committee, Gonabad University of Medical Sciences, Gonabad, Iran Marzieh.ebr74@gmail.com 00319475328460037761 00319475328460037761 No Masoumeh Fani Department of Anatomical Sciences, School of Medicine, Gonabad University of Medical Sciences, Gonabad, Iran m.fani2017@yahoo.com 00319475328460037762 00319475328460037762 No Seyed-Hosein Abtahi-Evari Department of Biochemistry, School of Medicine, Gonabad University of Medical Sciences, Gonabad, Iran Abtahi.h@gmu.ac.ir 00319475328460037763 00319475328460037763 No Balal Brazvan Department of Anatomical Sciences, School of Medicine, Gonabad University of Medical Sciences, Gonabad, Iran bbarazvan@gmail.com 00319475328460037764 00319475328460037764 No Sohrab Azin Student Research Committee, Gonabad University of Medical Sciences, Gonabad, Iran Sohrab.azin.75119@gmail.com 00319475328460037765 00319475328460037765 No Majid Shokoohi Department of Anatomical Sciences, Faculty of Medicine, Tabriz University of Medical Sciences, Tabriz, Iran a.shokoohy@yahoo.com 00319475328460037766 00319475328460037766 No Sajjad Abbasi Student Research Committee, Gonabad University of Medical Sciences, Gonabad, Iran Sajjadabbasi75@gmail.com 00319475328460037767 00319475328460037767 No vida Alikhani Department of Physiology, School of Medicine, Gonabad University of Medical Sciences, Gonabad, Iran vd.alikhani@gmail.com 00319475328460037768 00319475328460037768 No Amir- hosein Ebadi Student Research Committee, Gonabad University of Medical Sciences, Gonabad, Iran ebadi.amirhossein@hotmail.com 00319475328460037769 00319475328460037769 No Malihe soltani Department of Anatomical Sciences, School of Medicine, Gonabad University of Medical Sciences, Gonabad, Iran soltani.m@gmu.ac.ir 00319475328460037770 00319475328460037770 No Maryam Moghimian Department of Physiology, School of Medicine, Gonabad University of Medical Sciences, Gonabad, Iran moghimian.m@gmu.ac.ir 00319475328460037771 00319475328460037771 Yes

en Introduction: As a chronic neurological disorder, epilepsy is affected by social stress, which is one of the numerous complications in societies. In addition to medication, enriched environment (EE) and exercise are among the complementary strategies in the treatment of epilepsy. Oxidative stress, which potentially can activate the inflammatory pathways, is one of the causes of this disorder. So, we tried to examine thoroughly the beneficial impacts of EE and exercise on neuroinflammation in epileptic rats. Methods: Male Wistar rats were divided into five groups of twelve rats each, including: a control group, a group induced with pilocarpine to simulate epilepsy, an epileptic group subjected to social stress, an epileptic group placed in an enriched environment, and an epileptic group subjected to an exercise regimen. The impact of social stress, enriched environment, and exercise on oxidative stress biomarkers was investigated through TBARS  spectrophotometric test   and the gene expression of NLRP3, Caspase-1, IL18, and IL1β were evaluated through real-time PCR method. Results: Epilepsy and social stress caused a reduction in superoxide dismutase (SOD) and glutathione peroxidase (GPx) (p<0.05). Moreover, they resulted in an enhancement of plasma malondialdehyde (MDA), NLRP3, Caspase-1, interleukin-18 (IL18), and IL1β gene expression (p<0.05). Exercise increased the GPx and diminished the expression of Caspase-1 and IL-18 inflammatory genes (p<0.05). Accordingly, EE enhanced the SOD and GPx antioxidant indicators and reduced proinflammatory gene expression. Conclusion: In this research, social stress resulted in elevated levels of oxidative markers and upregulation of inflammatory gene expression. EE and regular exercise improved the situation. Temporal lobe epilepsy, social stress, enriched environment, exercise, NLRP3 pathway 194 204 http://ppj.phypha.ir/browse.php?a_code=A-10-2372-1&slc_lang=en&sid=1 2023/12/182024/09/292023/10/282024/07/12024/01/162024/04/272024/04/262024/02/192024/04/23 1403/2/4 2024/09/142024/12/82024/10/52024/09/252024/09/72024/08/312024/09/252024/10/52024/08/19 1403/5/29 Fatemeh Moradi Department of Physiology, School of Medicine, Zanjan University of Medical Sciences, Zanjan, Iran f.moradi@zums.ac.ir 00319475328460037772 00319475328460037772 No Mahsa Jazini Department of Physiology, School of Medicine, Zanjan University of Medical Sciences, Zanjan, Iran mahsa.jazini92@gmail.com 00319475328460037773 00319475328460037773 No Hadi Feizi Department of Physiology, School of Medicine, Zanjan University of Medical Sciences, Zanjan, Iran hfeizik@zums.ac.ir 00319475328460037774 00319475328460037774 No Mahin Ganjkhani Department of Physiology, School of Medicine, Zanjan University of Medical Sciences, Zanjan, Iran mganjkhani@zums.ac.ir 00319475328460037775 00319475328460037775 Yes

en Introduction: Azoospermia is a common cause of male infertility. The latest hope for more affordable and safer therapies comes from stem cell research. In this study, the effect of mesenchymal stem cells derived from Wharton’s jelly on spermatogenesis in azoospermic mice with scrotal hyperthermia was investigated. Methods: For the experimental study, 24 adult male mice were divided into four groups: 1. control (Cont), 2. scrotal hyperthermia (Hyp), 3. scrotal hyperthermia + DMEM (10μl) (Hyp/DMEM), 4. scrotal hyperthermia + conditioned medium (10 μl) (Hyp/CM). A temperature of 43 °C was applied for 20 minutes every other day for two weeks to induce hyperthermia. The mice in the experimental group received 10 µl of human Jelly Wharton’s Stem Cell-Conditioned Medium (hJWSC-CM) intraperitoneally every other day for 35 days after hyperthermia. Animals were sacrificed after the experiments to perform additional molecular, biochemical, and stereological analyses. Results: The results showed that CM significantly increased both the total amount of sperm and the number of testicular cells, including spermatids, primary spermatocytes, spermatogonia, Leydig cells, and Sertoli cells, compared with Hyp and Hyp/DMEM; in addition, the biochemical characteristics of testicular tissue were significantly higher in the Hyp/CM group compared with the hyperthermia-induced groups. Further analysis revealed that when the Hyp/CM group was compared with the Hyp and Hyp/DMEM groups, Sox9 protein expression and proliferative gene expression increased significantly and the percentage of apoptotic testicular cells and pro-inflammatory gene expression decreased significantly. Conclusion: These results suggest that realistic therapeutic approaches for reproductive and regenerative medicine could benefit from hWJSC-CM. Scrotal hyperthermia, Conditioned medium, Wharton's jelly, Spermatogenesis, Azoospermia 205 218 http://ppj.phypha.ir/browse.php?a_code=A-10-2315-1&slc_lang=en&sid=1 2023/12/182024/09/292023/10/282024/07/12024/01/162024/04/272024/04/262024/02/192024/04/232024/01/27 1402/11/7 2024/09/142024/12/82024/10/52024/09/252024/09/72024/08/312024/09/252024/10/52024/08/192024/09/7 1403/6/17 Atefeh Shirazi tehrani Department of Biology and Anatomical Sciences, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Tehran, Iran atefeh.shirazi@yahoo.com 00319475328460037776 00319475328460037776 No Hojjat-Allah Abbaszadeh Laser Applications in Medical Sciences Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Tehran, Iran hoomanabs@gmail.com 00319475328460037777 00319475328460037777 No Abbas Aliaghaei Hearing Disorders Research Center, Loghman Hakim Hospital, Shahid Beheshti University of Medical Sciences, Tehran, Tehran, Iran aghaei60@gmail.com 00319475328460037778 00319475328460037778 No Hamid Nazarian Department of Biology and Anatomical Sciences, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Tehran, Iran hamid.nazarian@gmail.com 00319475328460037779 00319475328460037779 No Azar Afshar Department of Biology and Anatomical Sciences, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Tehran, Iran azar.afshar8894@gmail.com 00319475328460037780 00319475328460037780 No Fakhroddin Aghajanpour Department of Biology and Anatomical Sciences, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Tehran, Iran fakhroddin72@gmail.com 00319475328460037781 00319475328460037781 No Reza Soltani Department of Biology and Anatomical Sciences, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Tehran, Iran rezasoltanikazemii@gmail.com 00319475328460037782 00319475328460037782 No Pourya Raee Department of Biology and Anatomical Sciences, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Tehran, Iran pourayarayi3@gmail.com 00319475328460037783 00319475328460037783 No Fatemeh Fadaei Fathabadi Department of Biology and Anatomical Sciences, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Tehran, Iran mrs.tehrani92@yahoo.com 00319475328460037784 00319475328460037784 Yes Mohammad-Amin Abdollahifar Department of Biology and Anatomical Sciences, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Tehran, Iran m_amin58@yahoo.com 00319475328460037785 00319475328460037785 No

en Introduction: Diabetes mellitus is a metabolic disease. This disease is almost associated with depression and impaired pain sensation. Stevia rebaudiana Bertoni (stevia) has a sweet taste and a useful effect in blood glucose control. This study was designed to evaluate the effect of Stevia and Nano-stevia on psychological problems (e.g. depression and pain) induced by diabetes. Methods: By a single intraperitoneal administration of streptozotocin, diabetes mellitus was induced in male Wistar rats. Stevia (20 mg/dl per day for 30 days) and Nano-stevia (20 mg/dl per day for 30 days) were administrated intra-gastrically in diabetic rats. Using the forced swim test (FST) and formalin test, depression and pain behaviors were measured, respectively.  Results: The results exhibited that male diabetic rats showed hyperglycemia, depressant-related behavior, and hyperalgesic response. On the other hand, intragastric usage of Stevia (20 mg/dl per day for 30 days) and Nano-stevia (20 mg/dl per day for 30 days) modified the hyperglycemia, depressant-related response, and hyperalgesic effect in diabetic rats. Nano-stevia indicated the maximum significant response rather than Stevia. Conclusion: The results of this study indicate that Stevia and particularly Nano-stevia could be beneficial in managing diabetes and its associated behavioral complications. Diabetes, Stevia, Nano-stevia, Depression-related behavior, Pain 219 231 http://ppj.phypha.ir/browse.php?a_code=A-10-496-5&slc_lang=en&sid=1 2023/12/182024/09/292023/10/282024/07/12024/01/162024/04/272024/04/262024/02/192024/04/232024/01/272023/12/30 1402/10/9 2024/09/142024/12/82024/10/52024/09/252024/09/72024/08/312024/09/252024/10/52024/08/192024/09/72024/09/25 1403/7/4 Neda Mousavi-Niri Department of Biotechnology, Tehran Medical Sciences, Islamic Azad University, Tehran, Iran Neda.mousaviniri@gmail.com 00319475328460037786 00319475328460037786 No Maryam Naseroleslami Department of Cellular and Molecular Biology, Tehran Medical Sciences, Islamic Azad University, Tehran, Iran m.naseroleslami@iautmu.ac.ir 00319475328460037787 00319475328460037787 No Elham Ghanimati Department of Cellular and Molecular Biology, Faculty of Advanced Science and Technology, Tehran Medical Sciences, Islamic Azad University, Tehran, Iran lham74gh@gmail.com 00319475328460037788 00319475328460037788 No Marzieh Moheb-Alian Department of Cellular and Molecular Biology, Faculty of Advanced Science and Technology, Tehran Medical Sciences, Islamic Azad University, Tehran, Iran. marziehalian@yahoo.com 00319475328460037789 00319475328460037789 No Faezeh Abdollah-pour Department of Cellular and Molecular Biology, Faculty of Advanced Science and Technology, Tehran Medical Sciences, Islamic Azad University, Tehran, Iran. faezeh.abdollahpour95@gmail.com 00319475328460037790 00319475328460037790 No Fatemeh Khakpai Department of Physiology, Tehran Medical Sciences, Islamic Azad University, Tehran, Iran khakpai@gmail.com 00319475328460037791 00319475328460037791 Yes