en
jalali
1390
7
1
gregorian
2011
10
1
15
3
online
1
fulltext
en
Role of nitric oxide on the electrophysiological properties of isolated rabbit atrioventricular node by extracellular field potential during atrial fibrillation
Introduction: The aim of the present study was to determine direct effects of NO modulation on protective
electrophysiological properties of atrioventricular node (AV node) in the experimental model of AF in rabbit.
Methods: Isolated perfused rabbit AV nodal preparations were used in two groups. In the first group (N=7), LNAME
(50μM) was applied. In the second group (N=12), different concentrations of L - argenine (250 μM - 5000 μM)
were added to the solution. Programmed stimulation protocols were used to quantify AV nodal conduction time,
refractoriness and zone of concealment. AF protocol was executed by software with coupling intervals (ranging from 75
– 125 msec).
Results: L-NAME had depressive effects on basic AV nodal properties. L-Arginine (250μM) had direct inhibitory
effects on nodal conduction time, Wenckebach and refractoriness. Significant increases in the number of concealed
beats were induced by L-Arginine (500 μM ). Number of concealed beats were increased from 700.7 ± 33.7 to 763 ±21
msec (P<0.05). Trend of zone of concealment prolongation in a frequency-dependent model was abrogated by Larginine
(250, 5000 μM).
Conclusion: NO at low concentration (in the presence of L-NAME) had facilitatory role on AV nodal properties,
but at high concentration (in the presence of L-arginine) enhanced protective role of AV node during AF. Biphasic
modulatory role of NO may affect protective behavior of AV node during AF.
Nitric Oxide, AV node, Electrophysiology, Concealed conduction, Atrial Fibrillation
295
307
http://ppj.phypha.ir/browse.php?a_code=A-10-245-10&slc_lang=en&sid=1
2011/01/12
1389/10/22
2011/09/2
1390/6/11
Vahid
Khori
Golestan Cardiovascular Research Center
vaph99@yahoo.com
00319475328460010055
00319475328460010055
No
Alimohammad
Alizadeh
Cancer research center tehran university of medical sciences tehran
alizadeh@yahoo.com
00319475328460010056
00319475328460010056
No
Ameneh
Navaiyan
Golestan Cardiovascular Research Center
navaiyan@yahoo.com
00319475328460010057
00319475328460010057
No
Mohsen
Nayebpour
tehran university of medical sciences tehran
m_nayeb_2000@yahoo.com
00319475328460010058
00319475328460010058
No
Mona
Porabouk
Golestan Cardiovascular Research Center
abook_8181@yahoo.com
00319475328460010059
00319475328460010059
No
Fakhri
Badaghabadi
Golestan Cardiovascular Research Center
nazari_2005@yahoo.com
00319475328460010060
00319475328460010060
No
Shima
Changizi
Golestan Cardiovascular Research Center
changizi@yahoo.com
00319475328460010061
00319475328460010061
No
Maryam
Rajaei
Golestan Cardiovascular Research Center
rajaei@yahoo.com
00319475328460010062
00319475328460010062
No
Hamidreza
Moheimani
Golestan Cardiovascular Research Center
moheamani@yahoo.com
00319475328460010063
00319475328460010063
No
Hamidreza
Yazdi
Golestan Cardiovascular Research Center
vaph99@yahoo.com
00319475328460010064
00319475328460010064
Yes
en
The effect of long term treatment of lowest effective dose of para-nonylphenol on viability, morphology and proliferation of rat bone marrow mesenchymal stem cells
Introduction: In this study, the effect of para-nonylphenol as an environmental pollutant on viability, morphology
and proliferation of bone marrow mesenchymal stem cells was investigated.
Methods: Bone marrow mesenchymal stem cells of rat were treated with the 0.5, 1, 2.5, 3.5 and 5 μM of paranonylphenol
for a period of 21 days, then the viability of the cells were estimated using trypan blue and MTT methods.
After choosing the effective dose, the integration of the DNA was investigated using comet assay and agarose gel
electrophoresis. Mechanisms of cell death were also investigated by TUNEL assay and presence of caspase activity.
Results: The results showed that para-nonylphenol caused significant dose dependent reduction of viability and
proliferation of the cells. Comet assay, agarose gel electrophoresis and TUNEL test showed that the DNA of the cells
were damaged and broken after treatment with 0.5 and 2.5 μM of para-nonylphenol. In addition, activated caspase-3
was observed in the cytoplasm of treated cells.
Conclusion: This study showed that a very low concentration of para-nonylphenol has drastic effects on bone
marrow mesenchymal stem cells. This chemical is used in formulation of cosmetics and detergents and therfore may
have detrimental effects on the viability and proliferation of stem cells.
apoptosis, caspase, mesenchymal stem cells, para-nonylphenol, TUNEL
308
317
http://ppj.phypha.ir/browse.php?a_code=A-10-358-1&slc_lang=en&sid=1
2011/01/122011/04/30
1390/2/10
2011/09/22011/08/7
1390/5/16
Mohammad Husein
Abnosi
Arak University
abnosi2002@yahoo.com
00319475328460010031
00319475328460010031
Yes
Malek
Soleimani Mehranjani
Arak University
M-Soleimani@araku.ac.ir
00319475328460010032
00319475328460010032
No
Sayed Mohammad Ali
Shariatzadeh
Arak University
s-shariatzadeh@araku.ac.ir
00319475328460010033
00319475328460010033
No
Majid
Mahdiyeh Najafabadi
Arak University
m-mahdiyeh@araku.ac.ir
00319475328460010034
00319475328460010034
No
Laila
Dehdehi
student
M-abnsoi@araku.ac.ir
00319475328460010035
00319475328460010035
No
en
Response to Morphine in a unicellular animal model (Paramecium caudatum)
Introduction: Response to morphine and role of Nitric Oxide (NO) on expression of morphine response has been
studied in vertebrates. But, little evidence is provided in the matter in earlier invertebrates. This investigation for the
first time evaluated the effect of NO on expression of morphine potency in Paramecium caudatum.
Methods: Animal after isolation from natural media and specific identification was cultured in laboratory. 1 ml of
the isolated medium including the animals was added into the Sedgwick– Rafter cell counter. One μl of drugs was
infused into the cell counter. Morphine (1-60 μg/μl) was infused into the cell and its effect was recorded throughout 0-
180 sec. L-Arginine (1-8 μg/μl), a NO precursor, was infused prior to morphine (2 μg/μl). The NO producing enzyme
was inhibited by preinfusion of L-NAME. Also the naloxone was used to show the involvement of the opioid receptors
in the signaling of morphine response. In control speciements distilled water was added solely.
Results: The Paramecia under the infusion of morphine were aggregated. The most aggregation rate was observed
at a relatively low dose of the drug (2 μg/μl). L-Arginine showed a positive effect on the response (p<0.001) whearas
the effect was blocked by preinfusion of the L- NAME. Naloxone showed an inhibitory effect to morphine response.
The activity of the NOS was shown by using the NADPH-diaphorase.
Conclusion: A sign of morphine potency in single–celled animal is the cell aggregation, and the present results are
showing the interaction of NO system with the opioidergic system in this line. On the other hand, concerning the
potentiation effect of L-arginine on morphine effective dose in the model, this finding may be useful in reducing of
morphine’s side effects in patients under the treatment of the drug, and in the drug economy as well.
Paramecium caudatum, Morphine Potency, Nitric oxide
318
329
http://ppj.phypha.ir/browse.php?a_code=A-10-502-1&slc_lang=en&sid=1
2011/01/122011/04/302011/02/26
1389/12/7
2011/09/22011/08/72011/08/4
1390/5/13
Seyed Sajad
Shahrokhi
Shahed university
sajad.shahrokhi@yahoo.com
0031947532846009750
0031947532846009750
No
Manije
Karami
Shahed university
karami@shahed.ac.ir
0031947532846009751
0031947532846009751
Yes
Bahram
Kazemi
Shahid Beheshti university- Medicin school
kazemi@sbmu.ac.ir
0031947532846009752
0031947532846009752
No
en
Decreased Uncoupling Protein 2 and 3 (UCP2 and UCP3) mRNA expression by endurance exercise training with and without chronic administration of nandrolone in rat heart
Introduction: The effect of regular exercise in decreasing the incidence of heart diseases is well known. The abuse
of anabolic androgenic steroids (AAS) has been associated with cardiovascular disorders. Uncoupling proteins (UCPs)
transport protons across the inner mitochondrial membrane thereby proton gradient can be diminished by the action of
UCPs. This process will result in the uncoupling of mitochondrial respiration from ATP production. The goal of this
study was to investigate whether UCP2 and UCP3 are involved in the mechanisms of AAS-induced cardiac damage in
the rat heart.
Methods: In the current study, adult male Wistar rats were divided into five groups (n=8): Control, vehicle,
nandrolone, exercise, exercise- nandrolone. Rats in the exercise groups were submitted to a progressive running
program on a treadmill, 5 days a week for 10 weeks. Rats in the nandrolone and exercise- nandrolone groups received a
weekly intramuscular injection of nandrolone decanoate (10 mg/kg), while those in the vehicle group received Arachiz
oil as vehicle. Relative mRNA expression of UCP2 and UCP3 were determined with real-time RT- PCR.
Results: The data showed that chronic administration of nandrolone significantly up-regulated UCP2 and UCP3
mRNA in rat heart and endurance training induced a decrease in the expression of UCP2 and UCP3 mRNA with or
without presence of nandrolone.
Conclusion: It may be concluded that chronic nandrolone treatment causes an increase in the expression of UCP2
and UCP3 mRNA. Thus, it might decrease energy metabolism efficiency by impairment of ATP production. Physical
activity may decrease the adverse effects of nandrolone by down-regulation of the UCP2 and UCP3 mRNA expression.
nandrolone, exercise, UCP2, UCP3
330
340
http://ppj.phypha.ir/browse.php?a_code=A-10-512-1&slc_lang=en&sid=1
2011/01/122011/04/302011/02/262011/04/17
1390/1/28
2011/09/22011/08/72011/08/42011/06/26
1390/4/5
Gholamreza
Bayat
Tarbiat Modares University
gbayat2005@yahoo.com
00319475328460010024
00319475328460010024
No
Sohrab
Hajizadeh
Tarbiat Modares University
Hajizads@modares.ac.ir
00319475328460010025
00319475328460010025
Yes
Mohammad
Javan
Tarbiat Modares University
mjavan@modares.ac.ir
00319475328460010026
00319475328460010026
No
Mahdi
Forouzandeh Moghaddam
Tarbiat Modares University
foroz@modares.ac.ir
00319475328460010027
00319475328460010027
No
Fatemeh
Safari
Tarbiat Modares University
sa_physio@yahoo.com
00319475328460010028
00319475328460010028
No
Hossein
Azizi
Tarbiat Modares University
azizihf@yahoo.com
00319475328460010029
00319475328460010029
No
Roham
Mazloom
Tarbiat Modares University
rohammazloom@yahoo.com
00319475328460010030
00319475328460010030
No
en
Inotropic and chronotropic effects of new cilostamide derivatives on isolated rat atria
Introduction: It was shown in a previous study, that MCPIP, a cilostamide derivative, increased atrial contraction
force without changing contraction rate. To improve this property, two new derivatives of cilostamide were synthesized
and their effects on PDE3 activity and isolated rat atria contraction were investigated.
Methods: The inhibitory effect of each compound on PDE3 enzyme was determined. Reserpine-treated rat atria
were separated and suspended in an organ bath containing Krebs-Henseleit buffer. The effects of each compound alone
or in combination with isoprenaline were assessed.
Results: Mc2 and mbc2 inhibited PDE3 activity with a potency lower than cilostamide, while they increased the
contraction force with a higher efficacy (percentage higher than base line, cilostamide=19±3%, P<0.05, mc2= 43±7%
and mbc2=34±5%, Pmbc2>cilostamide). Atrial contraction rate was increased in the presence of cilostamide or
isoprenaline, but was not changed with synthetic compounds. Furthermore, the effect of isoprenaline on the contraction
rate was inhibited by synthetic compounds (percentage higher than base line, isoprenaline=68±9%, +mc2=6±5% and
+mbc2=36±6%) and was not changed in the presence of cilostamide.
Conclusion: The synthetic compounds induced an increase in the atrial contraction force that was higher than
cilostamide and was not correlated to their PDE3 inhibition. These compounds potentiated the effect of isoprenaline on
the contraction force which excludes the possibility of their inhibitory effect on the receptor. In addition to PDE3
inhibition, other mechanisms are involved in producing the effects of these compounds, which are not clear and needs
further investigation.
Phosphodiesterase, cilostamide, atria, rat, inotropic effect
341
350
http://ppj.phypha.ir/browse.php?a_code=A-10-509-1&slc_lang=en&sid=1
2011/01/122011/04/302011/02/262011/04/172011/04/12
1390/1/23
2011/09/22011/08/72011/08/42011/06/262011/08/4
1390/5/13
Azar
Hosseini
Mashhad University of Medical Sciences
HoseiniA841@mums.ac.ir
0031947532846009743
0031947532846009743
No
Reza
Shafiee-Nick
Mashhad University of Medical Sciences
Shafieer@mums.ac.ir
0031947532846009744
0031947532846009744
Yes
Heydar
Parsaee
Mashhad University of Medical Sciences
Parsaeeh@mums.ac.ir
0031947532846009745
0031947532846009745
No
Hamid
Sadeghian
Mashhad University of Medical Sciences
Sadeghianh@mums.ac.ir
0031947532846009746
0031947532846009746
No
en
Effects of chronic low- dose treatment with cyclophosphamide on the rat testis
Introduction: Cyclophosphamide (CP) is used for the treatment of various cancers. In spite of its therapeutic
importance, a wide range of adverse effects such as reproductive toxicity has been observed following the
administration of this drug in human and experimental animals. In the current study, we have investigated the adverse
effects of CP on morphology and histology of testis rats.
Methods: Twenty-one male Wistar rats were selected and randomly divided into 3 groups. CP was used at a dose of
6.1 mg/kg/day, (i.p.) for 50 days. At the end of the treatment, the histological and biochemical changes in testis, as well
as sperm count and motility were assessed.
Results: Testicular weight, sperm count and motility as well as serum testosterone concentration were significantly
decreased whereas malondialdehyde (MDA) level was significantly increased in CP group compared with those in the
control and sham groups. In addition, histological studies of testis structure showed that seminiferous tubules of testis
were severely damaged in the CP group. CP increased the number of sloughing tubules and interstitial space, while it
decreased seminiferous tubular diameter (STD), seminiferous epithelial height (SE), tubule differentiation index (TDI)
and spermiation index (SPI).
Conclusion: The results suggest that cyclophosphamide affect fertility parameters and cause testis atrophy after
chronic treatment.
Chemotherapy, Cyclophosphamide, testis structure, sperm, rats
351
360
http://ppj.phypha.ir/browse.php?a_code=A-10-474-1&slc_lang=en&sid=1
2011/01/122011/04/302011/02/262011/04/172011/04/122011/04/30
1390/2/10
2011/09/22011/08/72011/08/42011/06/262011/08/42011/08/4
1390/5/13
Akram
Hosseini
hosseinia30@yahoo.com
00319475328460010051
00319475328460010051
Yes
Abbas
Ahmadi
ahmadiabbas36@yahoo.com
00319475328460010052
00319475328460010052
No
Firouz
Ghaderi pakdel
info@fgpakdel.com
00319475328460010053
00319475328460010053
No
Samad
Zare
s.zare@urmia.ac.ir
00319475328460010054
00319475328460010054
No
en
Role of Histaminegic and calcium channels in the inhibitory effects of hydroalcoholic extract of Matricaria recutita L. on isolated rabbit jejunum
Introduction: Considering the long traditional history of anti-inflammatory and anti-spasmodic effects of Matricria
spices on the gastrointestinal system, the present study aimed to investigate the role of calcium channels and Histamine
receptors in the inhibitory effects of hydroalcoholic dry extract of German chamomile (Matricaria recutita L.) on the
isolated rabbit jejunum.
Methods: All experiments were done on the isolated jejunum of New Zealand rabbits (1.8-2.5 kg). Dry extract of
aerial parts of M. recutita was obtained by the maceration technique. The study was performed on two groups (n=6 in
each group). In the first group, the effects of cumulative concentrations of M. recutita (3×10-3-1×10-2 mg/ml) on normal
and K+-induced contractions (50 mM) of isolated jejunum were studied. In the second group, the inhibitory role of M.
recutita ( 3 – 13×10-3 mg/ml) was evaluated in the presence and absence of histamine and cetrizine. In the presence and
absence of 10 μM certizine, a histamine H1-antagonist, a concentration-dependent inhibitory effect of M. recutita
extract in the range of 3-13×10-3 mg/ml was recorded the rabbit jejunum.
Results: Results showed that EC50 of M. recutita in the absence and presence of K+ was 6.3×10-3 and 6.5×10-
3mg/ml, respectively. IC50 values for two concentrations of M. recutita (8×10-3 , 1×10-2 ) to abrogated contractive
phase of Histamine was 9.55 × 10-6 and 1.57 × 10-6 μM. Cetrizine (10 μM) abolished inhibitory effects of M. recutita
(IC50=3.6×10-3), (p< 0.001).
Conclusion: Dry extract of matricaria recutita had inhibitory effects on the contractions of isolated rabbit jejunum.
Calcium channels and histamine were involved in these antispasmodic effects.
German chamomile hydroalcoholic Matricaria recutita L., Antispasmodic, Histamine receptor
361
370
http://ppj.phypha.ir/browse.php?a_code=A-10-245-9&slc_lang=en&sid=1
2011/01/122011/04/302011/02/262011/04/172011/04/122011/04/302010/11/27
1389/9/6
2011/09/22011/08/72011/08/42011/06/262011/08/42011/08/42011/08/4
1390/5/13
masoomeh
mazandarani
1Islamic Azad Univesity , Gorgan, Iran
mazandarani@yahoo.com
00319475328460010011
00319475328460010011
No
fatemeh
hoseini
Islamic Azad Univesity , Gorgan, Iran
hoseini@yahoo.com
00319475328460010012
00319475328460010012
No
akhtar
seifi
Golestan university of medical sciences, Gorgan, Iran
seifi@yahoo.com
00319475328460010013
00319475328460010013
No
hooman
bayat
Niak Pharmaceutical company
Bayat@yahoo.com
00319475328460010014
00319475328460010014
No
mona
pourabouk
Golestan Cardiovascular research center, Golestan university of medical sciences, Gorgan, Iran.
abook_8181@yahoo.com
00319475328460010015
00319475328460010015
No
fakhri
badaghabadi
Golestan Cardiovascular research center, Golestan university of medical sciences, Gorgan, Iran.
nazari_2005@yahoo.com
00319475328460010016
00319475328460010016
No
maryam
rajaei
Golestan Cardiovascular research center, Golestan university of medical sciences, Gorgan, Iran.
rajaei@yahoo.com
00319475328460010017
00319475328460010017
No
Hamidreza
Moheimani
00319475328460010018
00319475328460010018
No
vahid
khori
Golestan Cardiovascular research center, Golestan university of medical sciences, Gorgan, Iran.
vaph99@yahoo.com
00319475328460010019
00319475328460010019
Yes
en
Analgesic effect of morphine microinjected into the nucleus raphe magnus after electrolytic lesion of nucleus cuneiformis in tail-flick and formalin tests in rat
Introduction: The antinociceptive effect of morphine is, in part, mediated through the activation of a descending
pathway. One of the major components of this pathway is the nucleus raphe magnus (NRM). Our previous study
demonstrated the involvement of NRM in the analgesic effect of morphine microinjected into the nucleus cuneiformis
(NCF) in a descending manner. The aim of the current study was to investigate another aspect of the interaction
between these two nuclei in both acute and chronic inflammatory pain models.
Methods: In order to calculate 50% effective dose (ED50) of morphine, animals received bilateral morphine
injections (1, 2.5, 5 and 10 μg/0.5 μl saline) into the NRM. The obtained ED50 of morphine was applied into the NRM
with/without bilateral electrolytic lesion (500 μA, 30 sec) of the NCF. Tail-flick and formalin tests were applied as
behavioral analgesic tests in this study.
Results: Results interestingly showed that the intra-NRM morphine injection (ED50 1 μg/0.5 μl saline) resulted in
an increase in tail flick latencies (morphine-induced antinociception) at 30-min intervals, while bilateral electrolytic
lesions in the NCF could notably decreased the morphine-induced antinociception during 30-90 min after the injection
of morphine. Data also showed that bilateral morphine microinjected into the NRM, dose-dependently increases the
antinociceptive responses during both early and late phases of formalin test. The antinociceptive effect of morphine
microinjected into the NRM was significantly attenuated at the late phase but not early phase following the bilateral
destruction of NCF in formalin test.
Conclusion: It could be concluded that there is a reciprocal interaction between NRM and NCF during morphine -
induced antinociception in both acute and chronic inflammatory pain models in rat.
Nucleus raphe magnus, Nucleus cuneiformis, Tail-flick test, Formalin test, Electrolytic lesion, Pain
371
384
http://ppj.phypha.ir/browse.php?a_code=A-10-5-3&slc_lang=en&sid=1
2011/01/122011/04/302011/02/262011/04/172011/04/122011/04/302010/11/272011/06/6
1390/3/16
2011/09/22011/08/72011/08/42011/06/262011/08/42011/08/42011/08/42011/09/2
1390/6/11
Leila
Ahmad-Molaei
00319475328460010005
00319475328460010005
No
Mehdi
Ordikhani-Seyedlar
00319475328460010006
00319475328460010006
No
Maryam
Ziaei
00319475328460010007
00319475328460010007
No
Raha
Khademi
00319475328460010008
00319475328460010008
No
Pegah
Rouzmeh
00319475328460010009
00319475328460010009
No
Abbas
Haghparast
haghparast@yahoo.com
00319475328460010010
00319475328460010010
Yes
en
Effect of prolonged exposure to low-frequency electromagnetic fields on the interaction of nitrergic and cholinergic systems in the isolated rat trachea
Introduction: Nitric Oxide is an important endogenous compound that acts as bronchodilator in trachea.
Electromagnetic fields (EMF) affect nitric oxide level in different tissues. The interaction of nitrergic and cholinergic
systems in airways has been reported. Therefore, the present study was performed to evaluate the interaction of
cholinergic and nitrergic systems in the trachea of rats that have been exposed to EMF.
Methods: Adult male rats were divided into 3 groups: experimental group was exposed to 1 mT (militesla) EMF for
135 days, sham group was kept in the same condition but in off solenoid and control group was kept in normal
condition. After 135 days, the mechanical response of transverse rings of isolated trachea to acetylcholine in the
absence and presence of L-NAME, an inhibitor of nitric oxide synthesis, were recorded using PowerLab-AD system.
Results: The results showed a significant increase in cholinergic contractions of trachea in the presence of L-NAME
in control and sham groups (P< 0.05). However this enhancement of contraction was not detected in EMF exposed
group.
Conclusion: It can be concluded that nitric oxide has a modulatory effect on the airway cholinergic contraction,
however this modulation is inhibited by prolonged exposure to EMF.
electromagnetic field, trachea, nitrergic system
385
394
http://ppj.phypha.ir/browse.php?a_code=A-10-490-1&slc_lang=en&sid=1
2011/01/122011/04/302011/02/262011/04/172011/04/122011/04/302010/11/272011/06/62011/01/31
1389/11/11
2011/09/22011/08/72011/08/42011/06/262011/08/42011/08/42011/08/42011/09/22011/09/18
1390/6/27
Tahereh sadat
Javadifar
SHIRAZ UNIVERSITY
tjavadifar@yahoo.com
00319475328460010065
00319475328460010065
No
Aminollah
Bahaoddini
SHIRAZ UNIVERSITY
bahaodini@shirazu.ac.ir
00319475328460010066
00319475328460010066
Yes
Mohammad Ali
Ketabi
Shahid beheshti of medical science
maketabi21@yahoo.com
00319475328460010067
00319475328460010067
No
Firozeh
Gholampour
SHIRAZ UNIVERSITY
00319475328460010068
00319475328460010068
No
Hosein
Mirkhanni
shiraz university of medical sciences
00319475328460010069
00319475328460010069
No
en
Assesment of orexin receptor 1 in stress attenuated nociceptive behaviours in formalin test
Introduction: It is known that acute and chronic stress induce hormonal and neuronal changes which affecting both
pain threshold and nociceptive behaviours. Orexin plays an important role in modulation of pain and stress. Considering
pain modulation during stress and the role of orexin in pain and stress, orexin might be involved in pain modulation
during stress.We evaluated the involvement of orexin receptor-1in acute immobilization stress on the tonic pain model.
Methods: Adult male, Wistar rats (200–300 g), placed in a stereotaxic apparatus and canulla were inserted into their
left cerebral ventricle. After 1 week of recovery, animals were initially submitted to one session of acute restraint stress
(30 min) and immediately submitted to formalin injection in the hind paw to evaluate nociceptive behaviours. Orexin
receptor 1 antagonist (SB 334867) was injected intracerebroventricularly, 5 minute before formalin injection, while the
solvent was injected in the control group.
Results: two percent formalin produced typical biphasic pain responses in rats that was observed for more than 1
hour. Acute exposure to restraint stress reduced the nociceptive behaviour by chemical stimulation in phase 1,
interphase and phase 2. The short-term stress induced analgesia was reflected in a decrease in the nociceptive behaviour
during phase 1, whereas the long-term stress induced analgesia was reflected in a decrease in the nociceptive behaviours
during phase 2. Pretreatment with orexin receptor 1 antagonist (SB 334867) attenuated the antinociceptive behavioral
effect of restraint stress.
Conclusion: Our results indicate that orexin receptor 1 antagonist attenuated antinociceptive effect of restraint stress
assessed by formalin. These findings show that orexin receptor 1 might mediate an opioid-independent stress-induced
analgesia.
Acute Stress, pain, Formalin test, Rat, Orexin receptor 1
395
402
http://ppj.phypha.ir/browse.php?a_code=A-10-243-2&slc_lang=en&sid=1
2011/01/122011/04/302011/02/262011/04/172011/04/122011/04/302010/11/272011/06/62011/01/312011/05/31
1390/3/10
2011/09/22011/08/72011/08/42011/06/262011/08/42011/08/42011/08/42011/09/22011/09/182011/09/10
1390/6/19
Mohammad
Sofiabad
0031947532846009786
0031947532846009786
No
Nima
Heidari
0031947532846009787
0031947532846009787
No
Elmira
Ghasemi
0031947532846009788
0031947532846009788
No
Mohammad Hossein
Esmaeili
0031947532846009789
0031947532846009789
No
Hashem
Haghdoost-Yazdi
0031947532846009790
0031947532846009790
No
Elaheh
Erami
0031947532846009791
0031947532846009791
No
Hassan
Azhdari zarmehri
0031947532846009792
0031947532846009792
Yes
en
Comparative effects of daily and weekly boron supplementation on plasma steroid hormones and proinflammatory cytokines
Introduction: Boron possesses widespread properties and is important for human and animal nutrition. Since Boron
is rapidly bioavailable, the objective of the present study was to determine whether acute (hourly or daily), and weekly supplementationcould have any significant biological effects on the synthesis of steroids as well as inflammatory biomarkers.
Methods: Eight male volunteers participated in experiments on three occasions (day 0, 1 and 7). On the first day
(day 0), a blood sample was collected at 8.00 A. M, followed by ingestion of placebo. On the next day
(supplementation- day 1), similar procedure was followed by ingestion of 10 mg of boron capsule. On both occasions
samples of blood were collected every 2h for the next 6 h. Subjects consumed a capsule of 10 mg boron every day and
on day 7, blood collection was carried out again at 8.00 A.M. Independent sample t-tests were used to evaluate the
differences.
Results: Plasma boron was significantly increasedfollowing hourly (P=0.002) and weekly (P=0.000) consumption
of boron. After one week of supplementation, free testosterone levels were significantly increased (P<= 0.02) and
estradiol concentrations were significantly decreased (P<= 0.01). Dihydrotestosterone (DHT), cortisol and Vitamin D
showed slight non significant, increases. The ratios of free testosterone/testosterone (FT/T) (P<= 0.001), free
testosterone/estradiol (FT/E2) (P<= 0.004) and testosterone/estradiol (T/E2) (P<= 0.009) were significantly increased.
Also, all 3 inflammatory biomarkers were decreased after supplementation.
Conclusion: Although there are previous studies that report a decrease in proinflammatory cytokines induced by
boron consumption, to our knowledge, this is the first human study reporting an increase in plasma free testosterone
concentrations following consumption of a boron supplement. This indicates a possible protective role against diseases
of pathological conditions for this microelement.
Boron, Supplementation, Steroids, Cytokines
403
414
http://ppj.phypha.ir/browse.php?a_code=A-10-514-1&slc_lang=en&sid=1
2011/01/122011/04/302011/02/262011/04/172011/04/122011/04/302010/11/272011/06/62011/01/312011/05/312011/04/21
1390/2/1
2011/09/22011/08/72011/08/42011/06/262011/08/42011/08/42011/08/42011/09/22011/09/182011/09/102011/08/4
1390/5/13
Mohammad Reza
Naghii
Baqiyatallah (a.s.) University of Medical Sciences
naghiimr@yahoo.com
00319475328460010046
00319475328460010046
Yes
Mahmood
Mofid
00319475328460010047
00319475328460010047
No
Ali Reza
Asgari
00319475328460010048
00319475328460010048
No
Mahdi
Hedayati
00319475328460010049
00319475328460010049
No
Maryam Sadat
Daneshpour
00319475328460010050
00319475328460010050
No
en
Assesment of flaxseed on oxidative stress in prepubertal rats with experimental varicocele
Introduction: Perepubertal varicocele can result in hypotrophy of testes, sperm damage and decrease the function
of leydig cells in future. pathophysiology of varicocele is unclear. Increased reactive oxygen species (ROS) is a major
theory. There are many controversies in treatment of pediatric varicocele. Flaxseed (FS) is the richest source of lignans
with antioxidant properties. The aim of this study was to investigate effect of flaxseed on oxidative stress in prepubertal
rats with experimental varicocele.
Methods: 35 male prepubertal rats were divided into 5 groups: control, sham, sham that fed base diet which was
supplemented with 10% FS, varicocele, varicocele that fed base diet which was supplemented with 10% FS. Animals
were sacrificed six weeks later. Sperm superoxide anion and H2O2 production, MDA in testis and total antioxidant
capacity in semen were evaluated.
Results: Intracellular superoxide anion and H2O2 production was significantly higher in varicocele induced group
(P≤0.001), but FS significantly decreased them (P≤0.001). There was no significant difference for seminal plasma total
antioxidant activity among all groups (P≥0.05). Left testicular MDA concentration of rats with varicocele that were fed
by FS 10% was lower compared with varicocele groups (P≤0.05).
Conclusion: Fs as a fat soluble antioxidant can protect the sperm membrane from the damage induced by ROS
through its effective antioxidant potential.
Superoxide anion, stress oxidative, varicocele, flaxseed, Total antioxidant capacity
415
426
http://ppj.phypha.ir/browse.php?a_code=A-10-527-1&slc_lang=en&sid=1
2011/01/122011/04/302011/02/262011/04/172011/04/122011/04/302010/11/272011/06/62011/01/312011/05/312011/04/212011/06/8
1390/3/18
2011/09/22011/08/72011/08/42011/06/262011/08/42011/08/42011/08/42011/09/22011/09/182011/09/102011/08/42011/09/10
1390/6/19
Shahla
Sohrabipour
. Dept. Physiology
sh.sohrabipour@gmail.com
0031947532846009710
0031947532846009710
No
Adele
Jafari
. Dept. Physiology
jafari.adele@gmail.com
0031947532846009711
0031947532846009711
No
Mohamad
Kamalinejad
Dept. Pharmacogenosy
-MKAMALINEJAD@yahoo.com
0031947532846009712
0031947532846009712
No
, Abdolfatah
Sarrafnejd
Dept. Pathobiology
-sarrafnejad@tums.ac.ir
0031947532846009713
0031947532846009713
No
Taherah
Shahrestany
Dept. Pathobiology
-Minoo_sh2003@yahoo.com
0031947532846009714
0031947532846009714
No
Hamid-Reza
Sadeghipour
. Dept. Physiology
sadeghipour@tums.ac.ir
0031947532846009715
0031947532846009715
Yes
en
Evaluation of the effect of taurine on cisplatin-induced hepatic injury and oxidative stress in male rats
Introduction: The principal dose-limiting factor in the use of cisplatin as an antineoplastic drug is its hepatic
toxicity. This study was designed to investigate the protective role of taurine against cisplatin-induced hepatic injury.
Methods: Male albino rats (180-220 g) were divided in to 4 groups (n=8) as follows: (1) saline-treated group (2):
cisplatin-treated group (10 mg/kg ip) (3): group that received taurine (200 mg/kg ip) for 1hr before cisplatin (10 mg/kg
ip) administration (4): taurine treated group (200 mg/kg ip). After 7 days, the animals were sacrificed and blood
samples collected from the heart as well as liver tissues were kept at -70 °C till further analyses.
Results: analyses showed that cisplatin significantly increased ALT and AST serum levels (P<0.05) while pretreatment
with taurine resulted in the reduction of these markers. Catalase activity in cisplatin-treated rats was
significantly decreased (P<0.05) and taurine administration could recover this reduction. MDA content of the liver
tissue was significantly increased in cisplatin-exposed animals, while taurine treatment reduced the amount of MDA in
liver tissue.
Conclusion: Our data suggest that taurine prevents from cisplatin-induced hepatic injury and this effect may be due
to its antioxidant properties.
Cisplatin, Taurine, Hepatic injury, Antioxidant
427
434
http://ppj.phypha.ir/browse.php?a_code=A-10-438-1&slc_lang=en&sid=1
2011/01/122011/04/302011/02/262011/04/172011/04/122011/04/302010/11/272011/06/62011/01/312011/05/312011/04/212011/06/82011/02/23
1389/12/4
2011/09/22011/08/72011/08/42011/06/262011/08/42011/08/42011/08/42011/09/22011/09/182011/09/102011/08/42011/09/102011/08/7
1390/5/16
Maryam
Norozi Sarkarabad
mery_noruzi@ymail.com
00319475328460010070
00319475328460010070
Yes
Samad
Zare
00319475328460010071
00319475328460010071
No
en
Effect of nitric oxide on the attenuation of acquisition of morphine-induced conditioned place preference by the essential oil from Cuminum cyminum L. fruit in mice
Introduction: Nitric oxide (NO) is a neuronal messenger molecule in the central nervous system, which is
generated from L-arginine by nitric oxide synthase (NOS) and involves in many important opioid-induced effects. Our
previous studies revealed that Cuminum cyminum interestingly reduces morphine sensitization, tolerance and
dependency in male mice. Therefore, in the present study, the effect of intraperitoneal (ip) administration of different
doses of cumin fruit essential oil (FEO) on the acquisition of morphine-induced conditioned place preference (CPP) in
L-arginine treated mice was investigated.
Methods: In this study, the CPP paradigm was done on 231 adult male albino Wistar mice and conditioning scores
and locomotor activity were recorded by the Ethovision software.
Results: The results showed that solely administration of different doses of cumin FEO (0.01, 0.1, 0.5, 1 and 2%
ip) or L-arginine (50, 100 and 200 mg/kg ip) during CPP protocol could not induce CPP. Nonetheless, morphineinduced
CPP was significantly decreased by two higher doses of cumin FEO (1% and 2% P<0.05), while it was
increased by L-arginine (100 and 200 mg/kg) when they were injected before morphine (5 mg/kg) during the
acquisition period (P<0.001). Additionally, cumin FEO (0.01-2%) could interestingly attenuate the increasing effect of
L-arginine (200 mg/kg) on morphine-induced CPP in a dose-dependent manner.
Conclusion: In conclusion, it could be suggested that some components of cumin FEO attenuate the excessive
effect of L-arginine on morphine-induced CPP through inhibitory mechanisms on NO pathway. It seems that cumin
FEO possibly acts as a NOS inhibitor.
Cuminum cyminum, Nitric oxide, Morphine, L-Arginine, Conditioned place preference, Acquisition, Mice
435
443
http://ppj.phypha.ir/browse.php?a_code=A-10-5-2&slc_lang=en&sid=1
2011/01/122011/04/302011/02/262011/04/172011/04/122011/04/302010/11/272011/06/62011/01/312011/05/312011/04/212011/06/82011/02/232011/03/17
1389/12/26
2011/09/22011/08/72011/08/42011/06/262011/08/42011/08/42011/08/42011/09/22011/09/182011/09/102011/08/42011/09/102011/08/72011/09/2
1390/6/11
Pegah
Azizi
0031947532846009747
0031947532846009747
No
Mojtaba
Kermani
0031947532846009748
0031947532846009748
No
Abbas
Haghparast
haghparast@yahoo.com
0031947532846009749
0031947532846009749
Yes
en
Evaluation of the analgesic and anxiolytic effects of Dracocephalum polychaetum
Introduction: Dracocephalum polychaetum bornum is exclusively found in a limited geographical area in the
Kerman province It is used by the local people for treatment of abdominal pain, meteorism and musculoskeletal pain.
No study has been performed on the effects of D. polychaetum bornum, so the aim of this work was to assess the role of
the extract and essential oil of this plant on pain and anxiety assessed by formalin test and elevated plus-maze (EPM),
respectively, in male rats.
Methods: Analgesic effects: One hundred twelve NMRI male rats were divided into 14 groups. Aqueous extract
and essential oil were administered to 8 groups at doses of 25, 50, 100 and 200 mg/kg i.p., while 2 groups were treated
with normal saline, and the last 4 groups (sham positive) received ASA (300 mg/kg) and morphine (2.5 mg/kg).
Anxiolytic effect: Forty-two NMRI male rats were divided into 7 groups. Four groups were injected
intraperitoneally with 25, 50, 100 and 200 mg/kg of the extract of the plant and 2 groups were injected with normal
saline (control group) and 1 mg/kg diazepam (positive sham). Anxiolytic effect was evaluated by EPM.
Results: The results showed that the extract but not the essential oil at the dose of 200 mg/kg had a significant
analgesic effect 25, 30 and 35 minutes after administration. The findings on the anxiolytic effect revealed that there was
no significant difference between groups treated with different doses.
Conclusion: This study showed that D. polychaetum bornum had analgesic effects.
pain, anxiety, Dracocephalum polychaetum bornum, formalin test, elevated plus-maze
444
454
http://ppj.phypha.ir/browse.php?a_code=A-10-480-1&slc_lang=en&sid=1
2011/01/122011/04/302011/02/262011/04/172011/04/122011/04/302010/11/272011/06/62011/01/312011/05/312011/04/212011/06/82011/02/232011/03/172010/11/10
1389/8/19
2011/09/22011/08/72011/08/42011/06/262011/08/42011/08/42011/08/42011/09/22011/09/182011/09/102011/08/42011/09/102011/08/72011/09/22011/06/11
1390/3/21
Mojtaba
Khodami
00319475328460010072
00319475328460010072
No
Mehdi
Abbasnejad
mabbas@mail.uk.ac.ir
00319475328460010073
00319475328460010073
Yes
Vahid
Sheibani
00319475328460010074
00319475328460010074
No
Mina
Mobasher
00319475328460010075
00319475328460010075
No
Mitra
Mehrabani
00319475328460010076
00319475328460010076
No
Akbar
Anaie Goodary
00319475328460010077
00319475328460010077
No
Sahar
Salari
00319475328460010078
00319475328460010078
No