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Abstract:   (46 Views)
Introduction: The dorsomedial Periaqueductal Grey (dmPAG) is located around the cerebral aqueduct with various functions such as cardiovascular regulation and is affected by inflammation. Lipopolysaccharide (LPS) is a complex molecule that is known to affect blood pressure. The cardiovascular effect of LPS microinjection into the dmPAG was investigated.
Methods: Rats were divided into three groups consisting of 1) Control, 2) LPS (lipopolysaccharide, 50 ng), 3) LPS (lipopolysaccharide, 100 ng). The rats were mounted on a stereotaxic device after anesthesia, with a continuous recording of cardiovascular parameters being done by a PowerLab device, connected to a cannulated femoral artery. The changes (∆) in systolic blood pressure (SBP), diastolic blood pressure (DBP), mean arterial pressure (MAP), and heart rate (HR) were calculated at different times and compared to the control group.
Results: Both doses of LPS when microinjected into the brain brought on a significant hypotensive response in the pressure parameters (MAP, SBP, and DBP) when compared to control. A non-significant increase in HR was also documented in both groups.
Conclusion: The results of this experiment indicate that LPS, when microinjected into the dmPAG, induced a hypotensive response in anesthetized rats in both doses when compared to control.
Types of Manuscript: Original Research | Subject: Nervous system (others)