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Abstract:   (656 Views)
Introduction: Aminoglycoside antibiotics including gentamicin are used for treatment of gram-negative bacteria-induced infections; but gentamicin has severe side effects such as nephrotoxicity. Date palm pollen (DPP), as a herbal medicine is believed to have some anti-inflammatory and antioxidative stress effects. In this study the protective effect of DPP extract, after its phytochemical analysis, on gentamicin-induced nephrotoxicity were evaluated.
Methods: Wistar rats, allocated into 5 groups including control, sham, gentamicin, and two groups that received gentamicin along with DPP extract (200 or 400 mg/kg.BW). Plasma urea and creatinine concentrations were measured and oxidative stress was assessed by evaluating MDA, FRAP, CAT, and SOD. NF-κB, TNF-α, and ICAM-1 gene expression levels along with the leukocyte infiltration were measured for evaluating inflammation. Histopathological damages were also measured by studying H&E-stained tissue sections.
Results: The gentamicin receiving group had increased plasma urea and creatinine, increased MDA, and decreased FRAP, CAT and SOD activity in the kidney. The gentamicin administration also increased the TNF-α, NF-κB, and ICAM-1 gene expression, infiltration of leukocytes, and tissue damages in the kidney. DPP extract caused a partial or complete recovery of all these damages.
Conclusion: In conclusion, DPP extract protects the kidney against the side effects of gentamicin and improves its function and histopathological damages. The underlying mechanism is likely to decrease the NF-κB gene expression and consequently reducing proinflammatory cytokine genes expression, infiltration of leukocytes and oxidative stress. The DPP extract also increases the cellular antioxidant reserves.
     

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