Volume 11, Issue 3 (Fall 2007)                   Physiol Pharmacol 2007, 11(3): 238-243 | Back to browse issues page

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Najafi M, Gharakhani A, Ghavemi H, Eteraf Oskouei T. Protective effect of natural honey applied during ischemia and reperfusion on infarct size in ischemic heartheart. Physiol Pharmacol. 2007; 11 (3) :238-243
URL: http://ppj.phypha.ir/article-1-297-en.html
Abstract:   (16192 Views)
Introduction: In the current study, protective effect of natural honey applied during ischemia/reperfusion (I/R) was studied on infarct size in ischemic heartheart. Methods: Ischemic hearthearts (n=8 per group) were mounted on a Langendorff apparatus at constant pressure then subjected to 30 min regional ischemia followed by 120 min reperfusion. In control group, the hearts were perfused by a modified Krebs-Henseleit solution throughout the experiment, however, in the test groups they were perfused by Krebs solution enriched by natural honey (0.125, 0.25, 0.5 and 1%). At the end of reperfusion, the infarct size was determined by triphenyltetrazolium chloride and computerized planimetry methods. Results: The results showed that perfusion of ischemic hearthearts with natural honey produces significant reduction in infarct size and volume of infracted tissue. In the control group, infarct size was 45.6±3.4%, while honey (0.125, 0.25, 0.5 and 1%) reduced it to 14.8±5.1 (p<0.001), 24.6±7.3 (p<0.01), 31.4±7.3 (p<0.05) and 42.6±6.1% (p>0.05), respectively. In addition, infracted volume was lowered from 229±22mm3 (control) to 78±26 (p<0.001), 120±30 (p<0.01), 160±31 (p<0.05) and 201±45mm3 (p>0.05), respectively. Conclusion: The results of this study showed protective effects of natural honey against I/R injuries as reduction of infarct size. Probably, antioxidant activity of honey, scavenging of free radicals and presence of energy sources such as glucose are involved in its cardioprotective effect. Lower honey concentration seems to be more effective.
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