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Yarahmadi A, Mohammadi N, Zal F. Nicotine Effects on Enzymatic Antioxidant Defenses in Human Breast and Ovarian Cell Lines. Physiol Pharmacol. 2021;
URL: http://ppj.phypha.ir/article-1-1599-en.html
Abstract:   (182 Views)
Introduction: Nowadays, the prevalence of nicotine abuse among women has increased dramatically. In the current study, we aimed to investigate the effect of nicotine exposure on breast MCF-7 and ovarian OVCAR-3 cell lines for assessing the toxicity of nicotine in the cells of these organs.
Methods: MCF-7 and OVCAR-3 cells were treated with increasing nicotine concentrations ranging from 0 (control), 10-11, 10-8, and 10-6 M for 24 h. Effect of nicotine treatments on major antioxidant enzymes catalase (CAT), glutathione peroxidase (GPx), glutathione reductase (GR), cellular levels of glutathione (GSH), and Malondialdehyde (MDA) were monitored.
Results: We showed that the CAT activity in MCF-7 cells increased only at 10-6 M dose of nicotine. GPx and GR activity was decreased at 10-8 and 10-6 M of nicotine in MCF-7 cells, but in OVCAR-3 cells, this decrease was significant only at 10-6 M dose of nicotine. Reduced GSH decrease was statistically significant only at 10-8 and 10-6 M of nicotine in MCF-7 cells; otherwise, in OVCAR-3 cells, this decline was significant only at 10-6 M of nicotine. Nicotine at 10-8 and 10-6 M concentration caused a significant increase in MDA levels in MCF-7 cells.  
Conclusion: This study showed that breast MCF-7 cells are more vulnerable than ovarian OVCAR-3 cells against nicotine-induced oxidative toxicity.
     
Types of Manuscript: Original Research | Subject: Cellular and Molecular BioMedicine

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