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Abstract:   (479 Views)
Introduction: Sports activity increases PGC1α and Nrf2, the regulatory factors of mitochondrial biogenesis. This paper aims to study the impact of two-month sodium citrate supplementation with Moderate-Intensity Continuous Training (MICT) on PGC-1α and Nrf2 expression in diabetic rats.
Methods: Forty-five three-month-old male Wistar rats were haphazardly relegated to one of five equal groups(N=9): (1) healthy; (2) diabetic; (3) diabetes + exercise (DE); (4) diabetes+ supplementation (DS); and (5) diabetic + exercise + supplementation (DSE) and matched according to their weights. After induction, exercises began on a treadmill for 8 weeks, five days a week. The MICT protocol ran at 70% of its maximum speed for 36 minutes. The rats were sodium-citrate-supplemented at 15 mmol/L by drinking water for two months. PGC-1α and Nrf2 expression were measured through Western blotting in the soleus muscle. The data were analyzed using a univariate analysis of variance (ANOVA) and the Tukey post hoc test. Cohen’s D effect size (ES) was calculated to compare the groups.
Results: The results showed that induction of diabetes has significantly reduced the expression of PGC-1α (p< 0.001; ES=1.36) and Nrf2 (p<0.088; ES=0.24), while exercise has increased PGC-1α expression (p<0.001;ES=0.68). MICT activity/sodium citrate supplementation significantly increased the expression of PGC-1α in male diabetic rats. However, sodium citrate supplementation alone or in combination with MICT appears to have no obvious advantage over Nrf2 expression.
Conclusions: MICT activity and sodium citrate supplementation by increasing PGC-1α expression can be considered therapeutic strategies for diabetic patients. However, to increase Nrf2 expression, it is necessary to study different exercise intensities and doses of sodium citrate supplementation.


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