Abstract: (11081 Views)
Introduction: Fennel is rich in phytoestrogens and is used for estrogen deficiency disorders. Estrogens affect
anxiety through neurochemical systems such as GABA-A receptors. In this study the effects of fennel on GABA-A and
estrogen receptors in anxiety were investigated.
Methods: Adult female Wistar rats weighing (180±20 g) were divided into 8 groups. Groups received saline, fennel
(200, 500, 750 mg/kg), tamoxifen (15 mg/kg) + fennel (500 mg/kg), picrotoxin (1 mg/kg) + fennel (500 mg/kg). A
control group was also used. Elevated plus maze was used for evaluation of anxiety by measuring the time spent in the
open arm. All drugs were administered intraperitoneally.
Results: The results showed that fennel only at the dose of 500 mg/kg had significant anxiolytic effects and
increased the time spent in open arms (P<0.01). Picrotoxin (GABA-A antagonist) significantly prevented anxiolytic
effect of 500 mg/kg of fennel (P<0.001). Tamoxifen, an estrogen receptor antagonist, also abolished the anxiolytic
effect of fennel (P<0.001).
Conclusion: Fennel reduced anxiety in rats and picrotoxin, a non-competitive antagonist of GABA-A receptors, as
well as tamoxifen, an antagonist/agonist of estrogen receptors, reduced this anxiolytic effect. Thus fennel as a herbal
drug seems to have an anxiolytic effect and it probably acts through GABA-A and estrogen receptors.