%0 Journal Article %A Anjomshoaa, Maryam %A Karbalaei, Khadije %A Razavi, Shahnaz %A Mardani, Mohammad %A Tanhaei, Somayeh %A Nasr Esfahani, Mohammad Hossien %A Baharvand, Hossein %T Evaluation of effect of chick embryonic notochord on neural induction of mouse embryonic stem cells %J Physiology and Pharmacology %V 12 %N 1 %U http://ppj.phypha.ir/article-1-389-en.html %R %D 2008 %K Notochord, Neural induction, Embryonic stem cells, Co-culture, Retinoic acid, %X Introduction: The aim of this study was evaluate the ability of notochord to induce neural induction and/or differentiation of mouse embryonic stem cell to neuron and motor neuron, respectively. Methods: In order to produce embryoid bodies, ES cells line Royan B1 were grown in suspension in the absence of LIF for 4 days. EBs were divided into 4 groups. EBs in group 1 & 2 were further cultured in suspension for 4 days in presence of retinoic acid (RA) while EBs in group 3 and 4 were cultured in absence of RA. Unlike group 2 and 4, EBs in the group 1 and 3 were also co-cultured with notochord for further 4 days. Numbers and type of neurons were assessed for each group. Results: EBs in group 3 and 4 lead to 6 to 5 % neuron production while EBs in the group 1 and 2 lead to 55 and 42% neuron production, respectively. There were only significant difference at P %> http://ppj.phypha.ir/article-1-389-en.pdf %P 1-13 %& 1 %! %9 Experimental research article %L A-10-189-1 %+ %G eng %@ 24765236 %[ 2008