TY - JOUR JF - Physiol-Pharmacol JO - Physiol Pharmacol VL - 13 IS - 1 PY - 2009 Y1 - 2009/4/01 TI - BIO treatment enhances rat marrow-derived mesenchymal stem cell in vitro proliferation and viability TT - تیمار با BIO سبب افزایش میزان تکثیر و توان زیستی سلول های بنیادی مزانشیمی مشتق از مغز استخوان موش صحرایی در محیط کشت می شود N2 - Introduction: Previous investigations have indicated that the presence of BIO (6-Bromoindirubin-3-Oxime) in medium of some cell culture enhances the cell proliferation and viability. The aim of the present study was to investigate the BIO effects on in vitro expansion of rat marrow-derived mesenchymal stem cells (MSCs) culture. Methods: In the present experimental study, bone marrow cells from 7 rats were plated in the presence of 0.05, 0.01, 0.1, 1 and 1.5 µM of BIO and expanded through three successive subcultures. During the cultivation period, the cultures were statistically compared in terms of some indices of cell growth including the diameter and number of colonies, population doubling number (PDN) and the number of viable cells. Passaged-3 cells from all groups were examined whether or not they could differentiate into bone and adipose cells. Results: According to our results, the largest colonies were formed in the cultures with 0.1 and 1 µm BIO with diameter of respectively 1262.27±43.96 and 1335.71 ± 19.16 micrometer (P SP - 57 EP - 67 AU - Baghaban Eslaminejad, Mohamadreza AU - Salami, Farimah AU - Soleimani Mehranjani, Malek AU - Abnoosi, Mohamad-Hossein AU - Eftekhari-yazdi, Poopak AD - Stem Cell Department, Royan Institute KW - Mesenchymal stem cells KW - rat KW - BIO KW - proliferation. UR - http://ppj.phypha.ir/article-1-484-en.html ER -