Physiology and Pharmacology
Physiol Pharmacol
Medical Sciences
http://ppj.phypha.ir
32
journal32
24765236
24765244
10.61186/phypha
(previous ISSN: 17350581)
en
jalali
1399
12
1
gregorian
2021
3
1
25
1
online
1
fulltext
en
Anticonvulsant effect of acute curcumin nanoparticle on pentylenetetrazole-induced seizures in mice: non-involvement of JNK restoration
Nervous system (others)
Others
Experimental research article
Experimental research article
<div style="text-align: justify;"><strong>Introduction:</strong> Although several animal studies have indicated the antiepileptic effect for curcumin, there are reports stating the null antiepileptic effect of this substance. This inconsistency might be due to the low bioavailability of curcumin. Therefore, the current study aimed to assess the effect of oral bovine serum albumin (BSA)-based nanocurcumin on seizure caused by pentylenetetrazol (PTZ) in mice. Furthermore, due to the suggested involvement of JNK signaling in seizure pathology, the hippocampal pattern of JNK phosphorylation (activation) was evaluated. <strong>Methods:</strong> BSA based nanocurcumin was administered at doses of 50 and 100mg/kg oral gavage to male NMRI mice, one hour before PTZ administration. Intravenous PTZ paradigm was used to determine the threshold dose of PTZ to induce clonic seizures, while the intraperitoneal PTZ paradigm was applied to evaluate the latency for appearance of generalized clonus. Upon completion of intraperitoneal PTZ paradigm experiments, the hippocampi were removed and Western blot analysis was performed to determine the phosphorylated and total forms of JNK. <strong>Results:</strong> The results indicated that BSA-based nanocurcumin at the doses of 50 and 100mg/kg could significantly increase the threshold and latency of clonic seizure, which was a significant superior effect compared to natural curcumin. PTZ significantly increased the level of hippocampal JNK phosphorylation, but pretreatment of nanocurcumin did not modify this effect. <strong>Conclusion:</strong> The present study shows that converting curcumin to BSA-based nanocurcumin can increase its antiepileptic effect. Furthermore, the antiepileptic effect of nanocurcumin was not associated with a modification in PTZ-induced hippocampal JNK hyper activation.</div>
Curcumin, Nanoparticle, Seizure, Hippocampus, JNK, Pentylenetetrazol.
36
46
http://ppj.phypha.ir/browse.php?a_code=A-10-1085-4&slc_lang=en&sid=1
Leila
Moezi
moezil@sums.ac.ir
3200319475328460029158
3200319475328460029158
No
Nanobiology and Nanomedicine Research Centre, Shiraz University of Medical sciences, Shiraz, Iran
Nahid
Ashjazadeh
Ashjazn@sums.ac.ir
3200319475328460029159
3200319475328460029159
No
Clinical Neurology Research Center and Department of Neurology, Shiraz University of Medical Sciences, Shiraz, Iran
Shirin
Rezapanah
shirin.rezapanah@gmail.com
3200319475328460029160
3200319475328460029160
No
Clinical Neurology Research Center and Department of Neurology, Shiraz University of Medical Sciences, Shiraz, Iran
Fatema
Pirsalami
pirsalamif@sums.ac.ir
3200319475328460029161
3200319475328460029161
No
Clinical Neurology Research Center and Department of Neurology, Shiraz University of Medical Sciences, Shiraz, Iran
Zahra
Esmaeili
zesmaili1370@gmail.com
3200319475328460029162
3200319475328460029162
No
Nanobiology and Nanomedicine Research Centre, Shiraz University of Medical sciences, Shiraz, Iran
Roksana
SoukhakLari
roxana.sookhak@yahoo.com
3200319475328460029163
3200319475328460029163
No
Shiraz Neuroscience Research Centre, Shiraz University of Medical sciences, Shiraz, Iran
Maryam
Moosavi
marmoosavi@sums.ac.ir
3200319475328460029164
3200319475328460029164
Yes
Nanobiology and Nanomedicine Research Centre, Shiraz University of Medical sciences, Shiraz, Iran