Physiology and Pharmacology
Physiol Pharmacol
Medical Sciences
http://ppj.phypha.ir
32
journal32
24765236
24765244
10.61186/phypha
(previous ISSN: 17350581)
en
jalali
1401
3
1
gregorian
2022
6
1
26
2
online
1
fulltext
en
Acetylsalicylic acid enhanced neurotrophic profile of epidermal neural crest stem cells: a possible approach for the combination therapy
Cell, Stem Cell and Cancer
Cell, Stem Cell and Cancer
Experimental research article
Experimental research article
<div style="text-align: justify;"><span class="fontstyle0"><strong>Introduction:</strong> </span><span class="fontstyle2">Stem cell therapy is considered as a promising strategy to treat neurological disorders. Amongst different cell types that are recruited under these devastating conditions, epidermal neural crest stem cells (EPI-NCSCs) are known as potential candidates. Acetylsalicylic acid (ASA or aspirin) is one of the commonly prescribed drugs that might affect the therapeutic potential of the transplanted stem cells. Hence, the present study aimed to evaluate the effects of ASA on the expression of fundamental growth factors involved in restorative pathways expressed by EPI-NCSCs </span><span class="fontstyle3">in vitro </span><span class="fontstyle2">for possible combination therapy’s purpose. </span><span class="fontstyle0"><strong>Methods:</strong> </span><span class="fontstyle2">EPI-NCSCs were obtained from the rat’s hair follicle. The appropriate ASA concentration to treat the cells was defined based on the MTT assay and then the obtained cells were treated with 80 or 800µM ASA for 1, 3 or 7 days. The relative expressions of </span><span class="fontstyle3">Bdnf, Gdnf, Ngf, Neurotrophin-3, Vegf, Gfap, </span><span class="fontstyle2">and </span><span class="fontstyle3">doublecortin </span><span class="fontstyle2">were finally assessed by qRT-PCR. </span><span class="fontstyle0"><strong>Results:</strong> </span><span class="fontstyle2">The obtained data revealed that the growth factors expressions are influenced by concentration and duration of the treatment applied. One-day ASA treatment was found to be able to increase the expression of all the evaluated genes, except </span><span class="fontstyle3">Gdnf </span><span class="fontstyle2">and </span><span class="fontstyle3">doublecortin</span><span class="fontstyle2">, which elevated three days later. Herein, seven-day treatment of stem cells with 800µM ASA resulted in higher levels of </span><span class="fontstyle3">Bdnf, Vegf, </span><span class="fontstyle2">and </span><span class="fontstyle3">doublecortin</span><span class="fontstyle2">.</span><br />
<span class="fontstyle0"><strong>Conclusion:</strong> </span><span class="fontstyle2">Therefore, combination of aspirin and EPI-NCSCs might increase the therapeutic potential of these stem cells to treat neurological disorders.</span></div>
Aspirin, ASA, EPI-NCSCs, Growth factors, Neurological disorders
200
212
http://ppj.phypha.ir/browse.php?a_code=A-10-1543-1&slc_lang=en&sid=1
Seyedeh Shaghayegh
Zafarmand
3200319475328460030937
3200319475328460030937
No
Zarghan Branch, Islamic Azad University, Zarghan, Iran
Mohammad Saied
Salehi
3200319475328460030938
3200319475328460030938
No
Clinical Neurology Research Center, Shiraz University of Medical Sciences, Shiraz, Iran
Mohammad Javad
Mokhtari
3200319475328460030939
3200319475328460030939
No
Young Research and Elite Club, Zarghan Branch, Islamic Azad University, Zarghan, Iran
Anahid
Safari
3200319475328460030940
3200319475328460030940
No
Stem Cells Technology Research Center, Shiraz University of Medical Sciences, Shiraz, Iran
Sareh
Pandamooz
3200319475328460030941
3200319475328460030941
No
Stem Cells Technology Research Center, Shiraz University of Medical Sciences, Shiraz, Iran
Mehdi
Dianatpour
3200319475328460030942
3200319475328460030942
No
Stem Cells Technology Research Center, Shiraz University of Medical Sciences, Shiraz, Iran
Afshin
Borhani-Haghighi
neuro.ab@gmail.com
3200319475328460030943
3200319475328460030943
Yes
Clinical Neurology Research Center, Shiraz University of Medical Sciences, Shiraz, Iran