Physiology and Pharmacology
Physiol Pharmacol
Medical Sciences
http://ppj.phypha.ir
32
journal32
24765236
24765244
10.61186/phypha
(previous ISSN: 17350581)
en
jalali
1386
9
1
gregorian
2007
12
1
11
3
online
1
fulltext
en
Protective effect of natural honey applied during ischemia and reperfusion on infarct size in ischemic heartheart
Cardiovascular Physiology/Pharmacology
Cardiovascular Physiology/Pharmacology
Experimental research article
Experimental research article
Introduction: In the current study, protective effect of natural honey applied during ischemia/reperfusion (I/R) was studied on
infarct size in ischemic heartheart.
Methods: Ischemic hearthearts (n=8 per group) were mounted on a Langendorff apparatus at constant pressure then subjected to 30 min regional
ischemia followed by 120 min reperfusion. In control group, the hearts were perfused by a modified Krebs-Henseleit solution throughout the
experiment, however, in the test groups they were perfused by Krebs solution enriched by natural honey (0.125, 0.25, 0.5 and 1%). At the end of
reperfusion, the infarct size was determined by triphenyltetrazolium chloride and computerized planimetry methods.
Results: The results showed that perfusion of ischemic hearthearts with natural honey produces significant reduction in infarct
size and volume of infracted tissue. In the control group, infarct size was 45.6±3.4%, while honey (0.125, 0.25, 0.5 and 1%) reduced
it to 14.8±5.1 (p<0.001), 24.6±7.3 (p<0.01), 31.4±7.3 (p<0.05) and 42.6±6.1% (p>0.05), respectively. In addition, infracted volume
was lowered from 229±22mm3 (control) to 78±26 (p<0.001), 120±30 (p<0.01), 160±31 (p<0.05) and 201±45mm3 (p>0.05),
respectively.
Conclusion: The results of this study showed protective effects of natural honey against I/R injuries as reduction of infarct size.
Probably, antioxidant activity of honey, scavenging of free radicals and presence of energy sources such as glucose are involved in its
cardioprotective effect. Lower honey concentration seems to be more effective.
Natural honey, Infarct size, Ischemia/Reperfusion, Isolated heart, Rat
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243
http://ppj.phypha.ir/browse.php?a_code=A-10-168-1&slc_lang=en&sid=1
Moslem
Najafi
najafimoslem@yahoo.com
320031947532846004082
320031947532846004082
Yes
Tabriz University of Medical Sciences
Afshin
Gharakhani
320031947532846004083
320031947532846004083
No
Tabriz University of Medical Sciences
Hamed
Ghavemi
320031947532846004084
320031947532846004084
No
Tabriz University of Medical Sciences
Tahereh
Eteraf Oskouei
320031947532846004085
320031947532846004085
No
Tabriz University of Medical Sciences