Physiology and Pharmacology
Physiol Pharmacol
Medical Sciences
http://ppj.phypha.ir
32
journal32
24765236
24765244
10.61186/phypha
(previous ISSN: 17350581)
en
jalali
1389
10
1
gregorian
2011
1
1
14
4
online
1
fulltext
en
Existence of a delayed rectifier K+ current in the membrane of human embryonic stem cel
Others
Others
Experimental research article
Experimental research article
Introduction: Human embryonic stem cells (hESCs) are pluripotent cells that can proliferate and differentiate to
many cell types. Their electrophysiological properties have not yet been chracterzed. In this study, the passive
properties (such as resting membrane potential, input resistance and capacitance) and the contribution of delayed
rectifier K+ channel currents to the membrane conductance of hESCs was investigated.
Methods: hESC (Royan H6 line) was used in this study. Cells were cultured with feeder free culture method. To
study the electrophysiological properties of these cells, we used whole cell patch clamp technique in a voltage clamp
mode. Ionic currents were recorded by stimulating the cells with depolarizing steps from -90 mV to +50 mV. For
pharmacological determination of these currents, potassium channel blockers such as tetraethyl ammonium (TEA a
delayed rectifier K+ channel blocker) and 4-aminopyridine (4-AP as an A-type K+ channel blocker) were used.
Results: The resting membrane potential of hESCs was -8.66± 0.87 mV, the input resistance was 11.943 ± 0.23 MΩ
and the membrane capacitance was 1.46 ± 0.55 nF. In voltage clamp experiments, some outward currents were recorded
in hESCs that were progressively increased with positive voltages. These outward currents were inhibited by TEA but
not 4-AP. These channels did not show inactivation and their current were recorded from -60 mV. The mean
conductance of these channels was 11.81 ± 0.45 pS at -60 mV and 141.4 ± 10.97 pS at +50 mV. There were no inward
currents in hESCc.
These data show that hESCs have special electrophysiological properties. There are voltage dependent delayed rectifier K+ currents in these cells that are sensitive to TEA but not 4-AP.
349
357
http://ppj.phypha.ir/browse.php?a_code=A-10-51-13&slc_lang=en&sid=1
Sahar
Kiani
skiani@modares.ac.ir
320031947532846008225
320031947532846008225
No
Dept of Physiology, School of Medical Sciences, Tarbiat Modares University, Tehran, PO ?Box: 14115-331, Iran
Javad
Mirnajafi-Zadeh
mirnajaf@modares.ac.ir
320031947532846008226
320031947532846008226
Yes
Dept of Physiology, School of Medical Sciences, Tarbiat Modares University, Tehran, PO ?Box: 14115-331, Iran
Ebrahim
Shahbazi
320031947532846008227
320031947532846008227
No
Royan Institute for Stem Cell Biology and Technology
Hossein
Baharvand
Baharvand@RoyanInstitute.org
320031947532846008228
320031947532846008228
No
Royan Institute for Stem Cell Biology and Technology