en
jalali
1400
6
1
gregorian
2021
9
1
25
3
online
1
fulltext
en
Antimicrobial potential of a gel containing hydrogen peroxide and hyaluronic acid
No abstract provided as this is a letter to the editor
189
192
http://ppj.phypha.ir/browse.php?a_code=A-12-1448-1&slc_lang=en&sid=1
2020/09/18
1399/6/28
2021/01/31
1399/11/12
Jyothi
Tadakamadla
School of Medicine and Dentistry, Griffith University, Gold Coast, QLD, Australia
jyothi.tadakamadla@griffithuni.edu.au
00319475328460029267
00319475328460029267
Yes
Elisa
Boccalari
Department of Biomedical, Surgical and Dental Sciences, School of Dentistry, University of Milan; Milan, Italy
00319475328460029268
00319475328460029268
No
Cinzia
Maspero
Department of Biomedical, Surgical and Dental Sciences, School of Dentistry, University of Milan; Milan, Italy
cinzia.maspero@unimi.it
00319475328460029269
00319475328460029269
No
en
Molecular mechanisms and signaling pathways involved in immunopathological events of COVID-19
Introduction: COVID-19, a novel coronavirus that causes severe acute respiratory syndrome (SARS-CoV-2), is currently regarded as the most serious viral disease. During corona infection, viruses bind to host proteins and employ a variety of cellular pathways for their own purposes. Cell signaling is important for the regulation of cellular function. SARS-CoV-2 infection alters multiple signal transduction pathways that are critical for cell survival. The virus causes a severe and prolonged period of hypercytokinemia with misusing of these signaling cascades. Hyperactivation of the host immune system after infection with SARS-CoV-2 is the main cause of death in COVID-19 patients. Thus, to develop effective therapeutic approaches, it is necessary to first understand the problem and the underlying molecular pathways implicated in host immunological function/dysfunction. A number of intracellular signaling cascades have been implicated in infected cell pathways, including MAPK pathway, NF-κB pathway, JAK–STAT signaling pathway, PI3K/AKT/mTOR pathway and TLRI signaling cascades. Here, we have presented the molecular insights on the potential mechanisms involved in immunopathological events of COVID-19.
SARS-CoV-2 Infection, Signal transduction pathways, Cytokine storm.
193
205
http://ppj.phypha.ir/browse.php?a_code=A-11-1299-2&slc_lang=en&sid=1
2020/09/182021/04/13
1400/1/24
2021/01/312021/07/26
1400/5/4
Ali Asghar
Peyvandi
Hearing Disorders Research Center, Loghman Hakim Hospital, Shahid Beheshti University of Medical Sciences, Tehran, Iran
00319475328460029270
00319475328460029270
No
Somayeh
Niknazar
Hearing Disorders Research Center, Loghman Hakim Hospital, Shahid Beheshti University of Medical Sciences, Tehran, Iran
niknazar@sbmu.ac.ir
00319475328460029271
00319475328460029271
Yes
Fatemeh
Zare Mehrjerdi
Neurobiomedical Research Center, Shahid Sadoughi University of Medical Sciences, Yazd, Iran
00319475328460029272
00319475328460029272
No
Hojjat-Allah
Abbaszadeh
Hearing Disorders Research Center, Loghman Hakim Hospital, Shahid Beheshti University of Medical Sciences, Tehran, Iran
00319475328460029273
00319475328460029273
No
Shahrokh
Khoshsirat
Hearing Disorders Research Center, Loghman Hakim Hospital, Shahid Beheshti University of Medical Sciences, Tehran, Iran
00319475328460029274
00319475328460029274
No
Maryam
Peyvandi
Hearing Disorders Research Center, Loghman Hakim Hospital, Shahid Beheshti University of Medical Sciences, Tehran, Iran
00319475328460029275
00319475328460029275
No
en
A study of adverse drug reactions and potential drug-drug interactions in the patients attending the Psychiatric Outpatient Department in a Tertiary Care Teaching Hospital: a cross-sectional study
Introduction: The objective of this study was to record and analyze the adverse drug reactions (ADRs) due to psychotropic drugs and the potential drug-drug interactions (pDDIs) amongst different psychotropic drugs as well as pDDIs between psychotropic drugs and other co-prescribed drugs by using Medscape software (online). Methods: A cross sectional study was carried out in patients visiting the Psychiatric Outpatient Department of a Tertiary Care Teaching Hospital. Total 500 prescriptions were analysed for the ADRs and pDDIs. Results: Total 37 ADRs were observed in 32 (6.4%) patients. Antipsychotics was the most common group and olanzapine was the most common psychotropic drug suspected of causing ADRs. Tremors was the most common ADR observed. All of the ADRs were nonserious and were in a “Recovering” state when the data was collected. Total 1051 pDDIs were observed in all the 500 prescriptions surveyed, out of which 361 prescriptions were showing at least one pDDI. Conclusion: The overall incidence of ADRs was not very high (6.4%), which reiterates the judicious use of the drugs in the study setting. Majority of prescriptions had only 1-2 pDDIs per prescription.
Adverse drug reactions, Drug interactions, Psychotropic drugs, Olanzapine, Causality.
206
213
http://ppj.phypha.ir/browse.php?a_code=A-10-1452-1&slc_lang=en&sid=1
2020/09/182021/04/132020/09/27
1399/7/6
2021/01/312021/07/262021/01/17
1399/10/28
Adit
Deshmukh
Department of Pharmacology, B. J. Government Medical College and Sassoon General Hospital, Pune, India
00319475328460029276
00319475328460029276
No
Sangeeta
Dabhade
Department of Pharmacology, B. J. Government Medical College and Sassoon General Hospital, Pune, India
sangeetadr99@gmail.com
00319475328460029277
00319475328460029277
Yes
en
The impact of mild and short-term social stress on urinary levels of T3 and iodine in students of elementary schools
Introduction: Stress is a determinant factor in thyroid gland functions. A decrease in iodine concentration and thyroid hormones impairs cognitive functions in students. The responsiveness of thyroid hormones to stresses depends on many factors such as the intensity and duration of a particular stressor. This study aimed to examine the effect of mild and short-term social stress on heart rate (HR), blood pressure (BP), triiodothyronine (T3) and iodine concentrations of urine in students. Methods: This controlled before-after study was conducted on 200 students aged 9-12 years (100 girls and 100 boys). The HR and BP of the students were measured and their urine samples were collected before and after the intervention in both stressed and control students. To induce stress, the students were asked to read aloud a text from their own book in front of some audience. Results: In a mixed sample of girls and boys, a significant effect of stress was detected on BP and HR. There was no significant association between the stress and urinary level of T3 and iodine neither in girls nor in boys (and mixed sample). In girls, HR significantly increased in the stressed group while there was no significant connection between stress and BP. In boys, there was a significant difference between stress and control groups in terms of systolic and diastolic BP, but not the HR. Conclusion: Although the stress had a significant sex-specific impact on HR and BP, urinary levels of T3 and iodine were not affected in elementary students.
Blood pressure, Elementary schools, Iodine, Metabolic adjustments, Social stress, T3.
214
222
http://ppj.phypha.ir/browse.php?a_code=A-10-144-6&slc_lang=en&sid=1
2020/09/182021/04/132020/09/272020/03/30
1399/1/11
2021/01/312021/07/262021/01/172021/01/31
1399/11/12
Sakineh
Nouri Saeidlou
Food and Beverages Safety Research Center, Urmia University of Medical Science, Urmia, Iran
00319475328460029278
00319475328460029278
No
Ehsan
Saboory
Neurophysiology Research Center, Urmia University of Medical Sciences, Urmia, Iran
saboory@zums.ac.ir
00319475328460029279
00319475328460029279
Yes
Leila
Derafshpour
Neurophysiology Research Center, Urmia University of Medical Sciences, Urmia, Iran
00319475328460029280
00319475328460029280
No
Sima
Masudi
Department of Biostatistics and Epidemiology, School of Medicine, Urmia University of Medical Science, Urmia, Iran
00319475328460029281
00319475328460029281
No
Zafar
Gholinejad
Department of Biochemistry, School of Medicine, Urmia University of Medical Science, Urmia, Iran
00319475328460029282
00319475328460029282
No
Yousef
Rasmi
Department of Biochemistry, School of Medicine, Urmia University of Medical Science, Urmia, Iran
00319475328460029283
00319475328460029283
No
en
High-dose fluoxetine improved long-term potentiation of the hippocampal dentate gyrus in male rats
Introduction: Paradoxical data have been reported regarding the effects of fluoxetine on different types of learning and memory. Hippocampus-dependent memory is mediated by long-term potentiation (LTP). Here, we evaluated the effects of acute administration of fluoxetine on LTP induction in the hippocampal dentate gyrus of intact rats. Methods: Eighteen rats were divided into three groups: the control group received saline 15min before high-frequency stimulation (HFS) and the fluoxetine groups were treated with fluoxetine (2 or 10mg/kg), 15min before HFS. The rats were anesthetized with urethane and put in a stereotaxic system for surgery, electrode implantation and field recording. After ensuring a steady-state baseline response, a single intraperitoneal injection of saline or fluoxetine (2 or 10mg/kg) was done. Next, population spike amplitude, excitatory postsynaptic potential (EPSP) slope, and paired-pulse stimuli (to determine recurrent inhibitory interneuron) were measured in the hippocampal dentate gyrus in three groups. Results: The results showed that population spike amplitude markedly increased in the fluoxetine (2 and 10mg/kg) group than in the saline group. Also, EPSP slope induction in the fluoxetine (10mg/kg) group showed an increase, 60min after HFS compared with the control group. Fluoxetine did not significantly affect recurrent inhibition. Conclusion: These results indicated that the acute administration of high-dose fluoxetine (10mg/kg) can induce LTP. Thus, fluoxetine can be considered as a memory enhancer in intact rats.
Fluoxetine, Selective serotonin reuptake inhibitors, Long-term potentiation, Hippocampus.
223
230
http://ppj.phypha.ir/browse.php?a_code=A-10-1336-2&slc_lang=en&sid=1
2020/09/182021/04/132020/09/272020/03/302020/10/21
1399/7/30
2021/01/312021/07/262021/01/172021/01/312021/01/25
1399/11/6
Siamak
Shahidi
Department of Physiology, School of Medicine, Hamadan University of Medical Sciences, Hamadan, Iran
00319475328460029284
00319475328460029284
No
Leila
Rabiee
Department of Physiology, School of Medicine, Hamadan University of Medical Sciences, Hamadan, Iran
00319475328460029285
00319475328460029285
No
Alireza
Komaki
Department of Physiology, School of Medicine, Hamadan University of Medical Sciences, Hamadan, Iran
00319475328460029286
00319475328460029286
No
Reihaneh
Sadeghian
Medical Plants Research Center, Basic Health Sciences Institute, Shahrekord University of Medical Sciences, Shahrekord, Iran
sadeghian.r@skums.ac.ir
00319475328460029287
00319475328460029287
Yes
en
The effect of swimming exercise on thyroid function, spatial memory and anxiety in normal and propylthiouracil-induced hypothyroidism in Wistar rats
Introduction: Swimming exercises improve various nerve growth factors and angiogenesis that encouraged the researchers to investigate the effect of swimming exercises on thyroid function (cyclic adenosine monophosphate [cAMP] and dual oxidase 2 [DUOX2] in free T4 [FT4] secretion), spatial memory, behavior and anxiety on normal Wistar pups and those given with propylthiouracil (PTU). Methods: The subjects of this research were normal Wistar pups and those given 25 ppm PTU from the 1st week until the 12th week of age. Swimming activity was started in 4-weekold pups after acclimatization for 1 week. Swimming exercises were conducted with a load of 1–2% body weight and 30min duration per day, 5 times a week for 8 weeks. The levels of FT4 serum, cAMP and DUOX2 of the thyroid gland homogenate were measured using enzyme-linked immunosorbent assay. The rats’ ability to maintain spatial memory was measured using the Morris water maze and anxiety using the open field maze. Results: The FT4 levels significantly decreased after the administration of 25 ppm PTU for 3 weeks and it was much more decreased after 12 weeks administration. The administration of 25 ppm PTU for 12 weeks reduced the cAMP levels, increased DUOX2 and reduced the spatial memory skills and exploration behavior of rats. Swimming exercise increased and normalized these parameters. Conclusion: Swimming exercises for 8 weeks improve thyroid function, learning ability and spatial memory of normal and PTU-induced hypothyroid Wistar rats.
Anxiety, cAMP, FT4, Propylthiouracil, Spatial memory.
231
241
http://ppj.phypha.ir/browse.php?a_code=A-10-1389-2&slc_lang=en&sid=1
2020/09/182021/04/132020/09/272020/03/302020/10/212020/06/25
1399/4/5
2021/01/312021/07/262021/01/172021/01/312021/01/252020/12/13
1399/9/23
Zulkhah
Noor
Physiology Department, Faculty of Medicine and Health Sciences, Muhammadiyah University of Yogyakarta, Yogyakarta, Indonesia
capa.journal116@klinikjurnal.com
00319475328460029288
00319475328460029288
Yes
Denny
Agustiningsih
Physiology Department, Faculty of Medicine, Public Health and Nursing, Gadjah Mada University, Yogyakarta, Indonesia
00319475328460029289
00319475328460029289
No
Marsetyawan HNE
Soesatyo
Histology Department, Faculty of Medicine, Public Health and Nursing, Gadjah Mada University, Yogyakarta, Indonesia
00319475328460029290
00319475328460029290
No
Sri Kadarsih
Soejono
Physiology Department, Faculty of Medicine and Health Sciences, Muhammadiyah University of Yogyakarta, Yogyakarta, Indonesia
00319475328460029291
00319475328460029291
No
en
Topical formulation of tranilast improves hypertrophic scar in a rat model
Introduction: Hypertrophic scars are dermal complication that may lead to considerable morbidity. There is an intense medical need for novel therapies for this disease. Tranilast is an anti-allergic agent that clinically used for the treatment of keloids and hypertrophic scars. In this study, we aimed to evaluate the effect of tranilast 0.5% topical formulation on hypertrophic scars resulted by burn injury in rats. Methods: Burn wounds were inflicted on the skin area of the backs of all rats, following that the scars are created, treatment started and continued for 28 days. Animals were divided into two groups (n=8): the control scar group, which received a placebo and the treatment group that received 0.5% tranilast gel. After this period, skin biopsies were collected from each group for the following analyses. The tissue samples were analyzed by hematoxylin-eosin, Masson’s trichrome staining and qRT-PCR. Results: Histologically, topical tranilast reduced hypertrophic scar signs as compared to placebo. Tranilast treatment also resulted in a reduction in type I and III collagen, transforming growth factor-beta1, Smad2 and vascular endothelial growth factor , and increased Smad7 mRNA expression in the skin scar site. Conclusion: These findings showed that the 0.5% tranilast topical gel could be effective in the treatment of hypertrophic scars in a rat experiment.
Tranilast, Hypertrophic scar, Wound healing, Topical formulation.
242
250
http://ppj.phypha.ir/browse.php?a_code=A-10-1425-1&slc_lang=en&sid=1
2020/09/182021/04/132020/09/272020/03/302020/10/212020/06/252020/08/5
1399/5/15
2021/01/312021/07/262021/01/172021/01/312021/01/252020/12/132020/12/13
1399/9/23
Sara
Darakhshan
Pharmaceutical Sciences Research Center, Health Institute, Kermanshah University of Medical Sciences, Kermanshah, Iran
00319475328460029292
00319475328460029292
No
Fereshteh
Bagheri
Pharmaceutical Sciences Research Center, Health Institute, Kermanshah University of Medical Sciences, Kermanshah, Iran
00319475328460029293
00319475328460029293
No
Seyran
Kakabaraei
Department of Anatomical Sciences & Cell Biology, Kermanshah University of Medical Sciences, Kermanshah, Iran
00319475328460029294
00319475328460029294
No
Reza
Tahvilian
Pharmaceutical Sciences Research Center, Health Institute, Kermanshah University of Medical Sciences, Kermanshah, Iran
rtahvilian@kums.ac.ir
00319475328460029295
00319475328460029295
Yes
en
Protective effects of date palm pollen extract on gentamicin-induced hepatotoxicity
Introduction: Gentamicin, as an aminoglycoside antibiotic, is used to treat gram-negative bacterial infections. But despite its beneficial effects, gentamicin has side effects such as hepatotoxicity. Therefore, the aim of the present study was to investigate the protective effect of date palm pollen (DPP) hydroalcoholic extract against gentamicin-induced hepatotoxicity in rats. Methods: In present study the animals were divided into 5 groups, including control, sham, gentamicin and the two groups of gentamicin plus DPP extract at 200mg/kg and 400mg/kg. The plasma levels of aspartate aminotransferase (AST) and alanine aminotransferase (ALT) enzymes were measured to assess hepatic functional damages. Also, antioxidant enzymes activity including catalase (CAT) and superoxide dismutase (SOD) as well as total antioxidant capacity (FRAP) of the liver tissue sample were measured. Further, a tissue sample was fixed in 10% formaldehyde for hematoxylin and eosin staining and histopathological study. In the end of experiment, the animals were euthanized by deep anesthesia. Results: Gentamicin significantly increased the levels of plasma AST and ALT enzymes, caused histopathological damages, decreased CAT and SOD enzymes as well as FRAP in the liver tissue in comparison to the sham group. The concomitant administration of DPP hydroalcoholic extract and gentamicin with both examined doses could relatively improve these parameters, so that some parameters have not significant difference with the sham group. Conclusion: It can be concluded that the hydroalcoholic extract of DPP reduces histopathological damages, oxidative stress as well as hepatic enzymes following their increase by gentamicin.
Gentamicin, Date palm pollen, Hepatotoxicity, Oxidative stress, Histopathological damages.
251
260
http://ppj.phypha.ir/browse.php?a_code=A-10-1418-2&slc_lang=en&sid=1
2020/09/182021/04/132020/09/272020/03/302020/10/212020/06/252020/08/52020/08/2
1399/5/12
2021/01/312021/07/262021/01/172021/01/312021/01/252020/12/132020/12/132020/12/13
1399/9/23
Zeynab
Mohamadi Yarijani
Medical Biology Research Center, Kermanshah University of Medical Sciences, Kermanshah, Iran
00319475328460029296
00319475328460029296
No
Seyed Hamid
Madani
Molecular Pathology Research Center, Imam Reza Hospital, Kermanshah University of Medical Sciences, Kermanshah, Iran
00319475328460029297
00319475328460029297
No
Saeed
Changizi-Ashtiyani
Department of Physiology, Faculty of Medicine, Arak University of Medical Sciences, Arak, Iran
00319475328460029298
00319475328460029298
No
Houshang
Najafi
Medical Biology Research Center, Kermanshah University of Medical Sciences, Kermanshah, Iran
hnajafi@kums.ac.ir
00319475328460029299
00319475328460029299
Yes
en
Antioxidant activity, phenolic and flavonoid content of Lawsonia inermis and Haplophyllum vermiculare
Introduction: Continuous exposure of oxidants to the skin may disrupt the antioxidant balance and leads to inflammatory skin diseases (ISD). The aim of the present study was to compare the antioxidant activity, phenolic and flavonoid content of two traditionally used plants in ISD, Lawsonia inermis and Haplophyllum vermiculare. Methods: The hydroethanolic extract of the plants was prepared by maceration. Phenolic and flavonoid content of the extracts was measured respectively with Folin-Ciocateu and aluminum chloride methods. The monovalent reducing power and radical scavenging activity were also evaluated respectively by ferric reducing antioxidant power and 2,2-diphenyl-1-picryl-hydrazyl methods. Results: The reducing power of Lawsonia inermis (862.89±32.23 μmolFe2+/g) was significantly higher than Haplophyllum vermiculare extract (765.52±29.39 μmolFe2+/g). The radical scavenging activity of Lawsonia inermis extract at a concentration of 1000μg/ml (%65.72±0.77) was also significantly higher than Haplophyllum vermiculare (%36.34±2.52). The higher antioxidant activity of Lawsonia inermis is probably due to its higher phenolic (96.76±3.34μg GAE/mg) and flavonoid content (197.69±5.76μg QE/mg).
Conclusion: Henna leaves had higher antioxidant activity, phenolic and flavonoid content compared to aerial parts of Haplophyllum vermiculare, and may be more effective in improving oxidative stress, prevention and treatment of ISD.
Lawsonia inermis, Haplophyllum vermiculare, Antioxidative activity, Skin disease, Inflammation.
261
269
http://ppj.phypha.ir/browse.php?a_code=A-10-1402-1&slc_lang=en&sid=1
2020/09/182021/04/132020/09/272020/03/302020/10/212020/06/252020/08/52020/08/22020/06/17
1399/3/28
2021/01/312021/07/262021/01/172021/01/312021/01/252020/12/132020/12/132020/12/132020/12/13
1399/9/23
Alireza
Moulazadeh
Noncommunicable Disease Research Center, Fasa University of Medical Sciences, Fasa, Iran
00319475328460029300
00319475328460029300
No
Seyyed Amin
Kouhpayeh
Noncommunicable Disease Research Center, Fasa University of Medical Sciences, Fasa, Iran
00319475328460029301
00319475328460029301
No
Razieh
Ranjbar
Noncommunicable Disease Research Center, Fasa University of Medical Sciences, Fasa, Iran
00319475328460029302
00319475328460029302
No
Amin
Dakhili Ardestani
Student Research Committee, Fasa University of Medical Sciences, Fasa, Iran
00319475328460029303
00319475328460029303
No
Maryam
Hekmat
Student Research Committee, Fasa University of Medical Sciences, Fasa, Iran
00319475328460029304
00319475328460029304
No
Sara
Azarnia
Noncommunicable Disease Research Center, Fasa University of Medical Sciences, Fasa, Iran
s.azarnia@fums.ac.ir
00319475328460029305
00319475328460029305
No
Sohrab
Najafipour
Medicinal Plant Research Center, Fasa University of Medical Sciences, Fasa, Iran
najafipour@fums.ac.ir
00319475328460029306
00319475328460029306
Yes
en
The protective effects of apigenin on OLN-93 cellular model of cerebral stroke
Introduction: Cerebral stroke is known to be the third most common cause of death in the world. To study pathophysiology and effects of the therapeutic agents on stroke, the cellular model of stroke recently was used more. ONL-93, oligodendrocyte like cell, is known as an appropriate model to study the role of glial cells in stroke. Apigenin is a flavonoid that has neuroprotective and neurogenic effects; therefore, the purpose of this study was to investigate the role of apigenin flavonoid on the OLN-93 cell line in terms of oxygen and glucose deprivation in the cellular stroke model. Methods: The cells were divided into experimental, negative and positive control groups. Then, MTT, reactive oxygen species (ROS), annexin and propidium iodide as well as Western blotting assays were performed to evaluate the viability and apoptosis. Results: The results showed that there was a significant increase in the number of live cells following administration of 1μM of apigenin in experimental groups and also, there was a significant difference in the number of live cells between two doses of 1μM and 0.75μM of the apigenin. The amount of ROS produced at a concentration of 1μM apigenin was a significant decrease compared to the positive control group and apoptotic cells also decreased significantly. The results for the expression of P53 protein showed a significant reduction in experimental groups. Conclusion: Based on our results, apigenin could have beneficial effects through the reduction of P53 and ROS production.
Oxygen and glucose deprivation, Apigenin, Apoptosis, OLN-93 cell line.
270
278
http://ppj.phypha.ir/browse.php?a_code=A-10-1309-2&slc_lang=en&sid=1
2020/09/182021/04/132020/09/272020/03/302020/10/212020/06/252020/08/52020/08/22020/06/172020/09/9
1399/6/19
2021/01/312021/07/262021/01/172021/01/312021/01/252020/12/132020/12/132020/12/132020/12/132020/10/27
1399/8/6
Samira
Miraee
Department of Microbiology, Karaj Branch, Islamic Azad University, Karaj, Iran
00319475328460029307
00319475328460029307
No
Mona
Farhadi
Department of Microbiology, Karaj Branch, Islamic Azad University, Karaj, Iran
mona.farhadi@kiau.ac.ir
00319475328460029308
00319475328460029308
Yes
Seyed Behnamedin
Jameie
Neuroscience Research Center, Iran University of Medical Sciences, Tehran, Iran
00319475328460029309
00319475328460029309
No
Shabnam
Najafie
Neuroscience Research Center, Iran University of Medical Sciences, Tehran, Iran
00319475328460029310
00319475328460029310
No
en
Nicotine effects on enzymatic antioxidant defenses in human breast and ovarian cell lines
Introduction: Nowadays, the prevalence of nicotine abuse among women has increased dramatically. In the current study, we aimed to investigate the effect of nicotine exposure on breast MCF-7 and ovarian OVCAR-3 cell lines for assessing the toxicity of nicotine in the cells of these organs. Methods: The MCF-7 and OVCAR-3 cells were treated with increasing nicotine concentrations ranging from 0 (control), 10-11, 10-8 and 10-6 M for 24h. Effect of nicotine treatments on major antioxidant enzymes catalase (CAT), glutathione peroxidase (GPx), glutathione reductase (GR), cellular levels of glutathione (GSH) and malondialdehyde (MDA) were monitored. Results: We showed that the CAT activity in MCF-7 cells increased only at 10-6 M dose of nicotine. The GPx and GR activity was decreased at 10-8 and 10-6 M of nicotine in MCF-7 cells, but in OVCAR-3 cells, this decrease was significant only at 10-6 M dose of nicotine. Reduced GSH decrease was statistically significant only at 10-8 and 10-6 M of nicotine in MCF-7 cells; otherwise, in OVCAR-3 cells, this decline was significant only at 10-6 M of nicotine. Nicotine at 10-8 and 10-6 M concentration caused a significant increase in MDA levels in MCF-7 cells. Conclusion: This study showed that breast MCF-7 cells are more vulnerable than ovarian OVCAR-3 cells against nicotine-induced oxidative toxicity.
Nicotine, Antioxidant enzymes, Oxidative stress, MCF-7 cells, OVCAR-3 cells.
279
287
http://ppj.phypha.ir/browse.php?a_code=A-10-1357-1&slc_lang=en&sid=1
2020/09/182021/04/132020/09/272020/03/302020/10/212020/06/252020/08/52020/08/22020/06/172020/09/92020/04/1
1399/1/13
2021/01/312021/07/262021/01/172021/01/312021/01/252020/12/132020/12/132020/12/132020/12/132020/10/272020/12/26
1399/10/6
Amir
Yarahmadi
Department of Biochemistry, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran
00319475328460029311
00319475328460029311
No
Neda
Mohammadi
Department of Biochemistry, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran
00319475328460029312
00319475328460029312
No
Fatemeh
Zal
Department of Biochemistry, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran
Fatemehzal@sums.ac.ir
00319475328460029313
00319475328460029313
Yes