Iranian Society of Physiology and Pharmacology
Physiology and Pharmacology
24765236
24765244
18
3
2014
10
1
Effect of Cannabinoid Receptor Agonist WIN55, 212-2 on the Anxiety Induced by PTSD in Male Rats
259
270
EN
Malihe
Ghasemi
School of Biology, Damghan University, Damghan, Iran
ghasemi.malih@gmail.com
N
Kataneh
Abrari
School of Biology, Damghan University, Damghan, Iran
abrari@du.ac.ir
Y
Iran
Goudarzi
School of Biology, Damghan University, Damghan, Iran
irangoudarzi@du.ac.ir
N
Ali
Rashidy-pour
Dept. of Physiology, Learning and Memory Lab, and Research Center of Physiology, Semnan University of Medical Sciences, Semnan, Iran
arashidy_pour44@yahoo.com
N
Mahmoud
elahdadi salmani
School of Biology, Damghan University, Damghan, Iran
elahdadi@du.ac.ir
N
Introduction: Posttraumatic stress disorder is a severe anxiety disorder caused by exposure to traumatic events. The
aim of this study was to induce PTSD in rats and examine the effect of WIN55-212-2, a cannabinoid receptor agonist,
on anxiety.
Methods: SPS;S model was used to induce PTSD in 56 male Wistar rats. Rats were restrained for 2 h, immediately
followed by forced swimming for 20 min. After 15 min of recuperation, animals were exposed to diethyl ether until
they lost consciousness. Thirty min later, rats received an electric foot shock in a shock chamber. Rats which were in
the shock group, without experiencing SPS, only received an electrical foot shock. Animals received IP injections of
WIN (two doses) or vehicle, before tests, in 3 continuous days. For PTSD induction, conditioned fear response was
measured. Anxiety-like behavior was examined twice with elevated plus-maze.
Results: PTSD induction with SPS;S significantly decreased open arm time (OAT) and open arm entry (OAE)
parameter, as compared to the control. WIN (0.25 mg/kg) significantly increased OAT as compared with the control.
Conclusion: WIN (0.25 mg/kg) decreased the anxiety like behavior induced by PTSD, so it seems to have antianxiety
effect.
Cannabinoid receptors, Post-traumatic stress disorder, anxiety, WIN55-212-2
http://ppj.phypha.ir/article-1-1015-en.html
http://ppj.phypha.ir/article-1-1015-en.pdf
Iranian Society of Physiology and Pharmacology
Physiology and Pharmacology
24765236
24765244
18
3
2014
10
1
Effects of Hyperthyroidism on the Serum Levels of Vanadium and Manganese trace Elements in Rats
271
280
EN
Namdar
yousofvand
Dept. of Biology, Faculty of Science, Razi University, Kermanshah, Iran
yousofnam@yahoo.com
Y
Fatemah
Abbasitabar
Dept. of Biology, Faculty of Sciences, Hamedan Branch of Islamic Azad University, Hamedan, Iran
N
Zahra
Salimi
Dept. of Biology, Faculty of Science, Razi University, Kermanshah, Iran
zahra.salimi.bio@gmail.com
N
Introduction: Trace elements are important for the normal function of thyroid and prevention of its diseases.
Normal activities of thyroid including the synthesis of thyroid hormones and regulation of metabolism are dependent on
the presence of trace elements. On the other hand, thyroid hormones may also affect the trace element levels. In this
study, we investigated the effects of induced hyperthyroidism by two different doses of levothyroxine on serum levels
of manganese and vanadium.
Methods: The study was performed on 24 male Wistar rats that were divided into 3 groups: Control group received
drinking water. The treated groups received either a low dose (4 mg per liter) or a high dose of levothyroxine (12 mg
per liter) in their drinking water for 35 days. At the end of the experiments, the animals were sacrificed under deep
anesthesia and blood samples were obtained. Serum levels of hormones (T3 andT4) were measured by RIA1.The
concentration of the trace elements manganese and vanadium in the serum was determined with IPC-OES2.
Results: Serum levels of T3,T4, manganese and vanadium, were significantly higher in hyperthyroidism groups that
received either high or low doses of drug compared with control (P<0.001). These increases were significantly
dependent on the dose of levothyroxine.
Conclusion: Our data suggest that hyperthyroidism induced by levothyroxine can increase serum levels of
manganese and vanadium. These changes were dependent on the dose of levothyroxine.
hyperthyroidism, levothyroxine, manganese, vanadium, rats
http://ppj.phypha.ir/article-1-982-en.html
http://ppj.phypha.ir/article-1-982-en.pdf
Iranian Society of Physiology and Pharmacology
Physiology and Pharmacology
24765236
24765244
18
3
2014
10
1
Neuroprotective effect of minocycline on PTZ-induced epileptiform activity and alterations of the shape of action potentials in snail neurons assessed by using intracellular recordings
281
291
EN
Mahtab
Bahrami
Dept. of Physiology, Medical School, International Branch, Shahid Beheshti University of Medical Sciences, Tehran, Iran
m.bahrami9091@yahoo.com
N
Zahra
Ghasemi
Neurophysiology Research Center and Dept. of Physiology, Medical School, Shahid Beheshti University of Medical Sciences, Evin, Tehran, Iran
ghasemi.z1985@gmail.com
N
Mahyar
Janahmadi
Neurophysiology Research Center and Dept. of Physiology, Medical School, Shahid Beheshti University of Medical Sciences, Evin, Tehran, Iran
mjanahmadi@yahoo.com
Y
Introduction: Epilepsy is a neurological disorder that affects 1-2% of the world population and about 30% of
patients are resistant to antiepileptic drug therapy. Therefore, new treatment alternatives are needed. In the present
study, the possible neuroprotective effect of minocycline against epileptiform activity induced by pentylenetetrazole
(PTZ) was assessed.
Methods: Conventional intracellular recordings were taken from F1 cells of Helix aspersa, under the current clamp
condition. Following extracellular application of minocycline alone (300 and 600 μM) or in combination with PTZ (25
mM), alterations in the firing pattern and action potential wave forms were studied and compared to the control group
and the group treated with PTZ alone.
Results: In the presence of PTZ alone (25mM), neurons displayed paroxysmal depolarization shift (PDS)-like
events and produced burst activity. When PTZ was applied following minocycline pretreatment, it did not produce burst
activity, and even reduced the firing frequency. In addition, pre-exposure to minocycline prevented almost some of the
changes in the AP shape induced by PTZ treatment.
Conclusion: The findings indicated that minocycline may be able to exert preventive effects against induction of
epileptiform activity and could be possibly considered as a new treatment option for epilepsy.
Minocycline, Epileptiform activity, Pentylenetetrazole (PTZ), Snail neurons, Intracellular recording
http://ppj.phypha.ir/article-1-1029-en.html
http://ppj.phypha.ir/article-1-1029-en.pdf
Iranian Society of Physiology and Pharmacology
Physiology and Pharmacology
24765236
24765244
18
3
2014
10
1
Cytoplasmic acidification reduces potassium channel activities in the endoplasmic reticulum of rat hepatocytes
292
303
EN
Naser
Khodaee
Dept. of Physiology, Medical School, Shahid Beheshti University of Medical Sciences, Tehran, Iran
naskod45@yahoo.com
N
Maedeh
Ghasemi
Dept. of Physiology, Medical School, Shahid Beheshti University of Medical Sciences, Tehran, Iran
ghasemi.m.ph@gmail
N
Fahanik-Babaei
Javad
Dept. of Physiology, Medical School, Shahid Beheshti University of Medical Sciences, Tehran, Iran
N
Reza
Saghiri
Dept. of Biochemistry, Pasteur Institute of Iran, Tehran, Iran
saghiri@pasture.ac.ir
N
Afsaneh
Eliasi
Dept. of Biochemistry, Pasteur Institute of Iran, Tehran, Iran
af.eliassi@sbmu.ac.ir
Y
Introduction: Intracellular pH (pHi) regulates essentially all aspects of cellular activities. However, it is unknown
how endoplasmic reticulum (ER) potassium channels sense pHi. In this study, we investigate the direct effects of pHi on
ER potassium channels.
Methods: We used channel incorporation into the bilayer lipid membrane method. L-α-phosphatidylcholine, a
membrane lipid, was extracted from fresh egg yolk. Bilayer lipid membrane was formed in a 150 μm diameter hole.
Rough endoplasmic reticulum vesicles were obtained from the liver after homogenization and several centrifugations.
All single channel recordings were filtered at 1 kHz and stored at a sampling rate of 10 kHz for offline analysis by
PClamp10. The purity of cell fractions was confirmed by western blot using specific markers of mitochondria, plasma
membrane, endoplasmic reticulum, and Golgi. The pH was measured with a pH meter (0.001 unit accuracy). Statistical
analysis was performed based on Markov noise free single channel analysis.
Results: Western blotting and antibodies directed against various cellular proteins revealed that ER fractions did not
contain specific proteins of the other subcellular compartments. Single channel recordings revealed a 596 pS K+
channel, which was inhibited by 2.5 mM ATP, 100 μM glibenclamide and 400 μM tolbutamide. No effect of increasing
pHi to 8.2 was found but decreasing pHi to below about 6.7 produced a marked inhibition of channel activity, with
complete block being observed at pHi 6.2.
Conclusion: Our results indicate that intracellular acidification inhibits ER K+ channel activities. The direct
regulation of ER K+ channels by pHi has important implications for ER homeostasis.
Cytoplasmic pH, KATP channel, bilayer lipid membrane, endoplasmic reticulum, hepatocyte
http://ppj.phypha.ir/article-1-1016-en.html
http://ppj.phypha.ir/article-1-1016-en.pdf
Iranian Society of Physiology and Pharmacology
Physiology and Pharmacology
24765236
24765244
18
3
2014
10
1
A molecular study on the endoplasmic reticulum potassium channels in hepatocytes
304
314
EN
Maedeh
Ghasemi
Dept. of Physiology, Medical School, Shahid Beheshti University of Medical Sciences, Evin, Tehran, Iran
ghasemi.m.ph@gmail.com
N
Naser
Khodaee ataloo
Dept. of Physiology, Medical School, Shahid Beheshti University of Medical Sciences, Evin, Tehran, Iran
naskod45
N
Fariba
Khodagholi
Neurobiology Research Center, Shahid Beheshti University of Medical Sciences, Evin, Tehran, Iran
khodagholi@sbmu.ac.ir
N
Afsaneh
Eliassi
Dept. of Physiology, Medical School, Shahid Beheshti University of Medical Sciences, Evin, Tehran, Iran
af.eliassi@sbmu.ac.ir
Y
Introduction: It has recently been suggested that the KATP channel subunits Kir6.x and BKCa channels exist in the
endoplasmic reticulum of cardiomyocytes and neurons. Our previous studies showed the electrophysiological behavior
of cation channels in the rough endoplasmic reticulum (RER) of rat hepatocytes. Therefore, we hypothesized that KATP
channels and Ca2+-activated potassium channels may exist in RER of the liver.
Methods: Ion channel incorporation of RER of hepatocytes into the bilayer lipid membrane and western blotting
allowed the identification of the rerKATP and Ca2+-activated potassium channels of intermediate conductance (rerIKCa
channel).
Results: Single channel recordings revealed a 211 pS K channel, which was sensitive to 200 nM charibdotoxin. In
addition, adding 2.5 mM ATP, 100 μM glibenclamide and 400 μM tolbutamide inhibited a 596 pS potassium channel
activity. According to the results of western blot analysis, Kir6.2, SUR1 and/or SUR2B, SUR2A and IKCa were
expressed in the endoplasmic reticulum fractions.
Conclusion: The data obtained in this study demonstrate, in part, the composition of rerKATP channel and the
presence of rerIKCa in the endoplasmic reticulum of the liver. These results will be useful for further analysis of the
composition of rerKATP and rerIKCa channels and help to understand the role of these channels in diseases.
endoplasmic reticulum, KATP channel, IKCa channel, hepatocyte
http://ppj.phypha.ir/article-1-1012-en.html
http://ppj.phypha.ir/article-1-1012-en.pdf
Iranian Society of Physiology and Pharmacology
Physiology and Pharmacology
24765236
24765244
18
3
2014
10
1
Effects of remote limb ischemic preconditioning on pulmonary function tests and maximal oxygen uptake
315
326
EN
Zahra
Akbari
Dept. of Physiology, Faculty of Medicine, Bushehr University of Medical Sciences, Bushehr, Iran
dr.zaakbari@gmail.com
N
Zahra
Sedaghat
Dept. of Physiology, Faculty of Medicine, Bushehr University of Medical Sciences, Bushehr, Iran
sedaghat.biology@yahoo.com
N
Mansour
Esmaili-Dehaj
Dept. of Physiology, Faculty of Medicine, Shahid Sadoughi University of Medical Sciences, Tehran, Iran
med1354@yahoo.com
N
Esmat
Karamean
Dept. of Exercise Physiology, Islamic Azad University, Jahrom Branch, Jahrom, Iran
esmatkarameam@yahoo.com
N
Narjes
Zarei
Dept. of Physical Education and Sport Sciences, Islamic Azad University, Bushehr Branch, Bushehr, Iran
N
Abbas
Keshavarzi
Dept. of Physiology, Faculty of Medicine, Bushehr University of Medical Sciences, Bushehr, Iran
keshavarzi2020@gmail.com
N
Khalil
Pourkhalili
Dept. of Physiology, Faculty of Medicine, Bushehr University of Medical Sciences, Bushehr, Iran
kh_pourkhalili@yahoo.com
Y
Introduction: Ischemic preconditioning (IPC) protects skeletal muscles from ischemia-reperfusion injury and
improves physical exercise performance. The purpose of the present study was to determine whether application of
remote ischemic preconditioning (RIPC) of upper limbs would affect the pulmonary function tests and the maximal
oxygen consumption (VO2max).
Methods: Twenty healthy trained and untrained subjects were examined under 2 experimental conditions of control
and RIPC groups. All individuals attended the laboratory twice, once as the control group and the next time as the RIPC
group in a counterbalanced order. These visits were at least 1 week apart and were taken place at the same time of the
day. RIPC was induced using a protocol of three cycles of 5 min ischemia/5 min reperfusion in both arms. Pulmonary
function tests and oxygen saturation (SPO2) were measured before and after the RIPC protocol. VO2max was estimated by
the Queen Step Test.
Results: Analysis of data revealed that RIPC increased FEV1, FEF25-75 and MVV tests in the untrained group,
while it increased FVC, FEV1, FEF25-75 and MVV tests in the trained group. Preconditioning also increased VO2max
and the maximal heart rate in trained subjects.
Conclusion: These results show that pre-exercise induction of limb ischemic preconditioning improves pulmonary
function tests and VO2max especially in trained subjects. Thus, this technique may be appropriate for the enhancement of
exercise performance in athletes during competitions and also for improving the respiratory function in different
pulmonary diseases in the near future.
Ischemic preconditioning, VO2max, Pulmonary function tests
http://ppj.phypha.ir/article-1-1021-en.html
http://ppj.phypha.ir/article-1-1021-en.pdf
Iranian Society of Physiology and Pharmacology
Physiology and Pharmacology
24765236
24765244
18
3
2014
10
1
Effect of intrahippocampal L-NAME injection on passive avoidance memory in adult male rats exposed to restraint stress
327
336
EN
Zahra
Moaiedfard
Dept. of Biology, Faculty of Science, Shahid Chamran University of Ahwaz, Ahwaz, Iran.
zmoayedfard@yahoo.com
Y
Hooman
Eshagh Harooni
Dept. of Biology, Faculty of Science, Shahid Chamran University of Ahwaz, Ahwaz, Iran.
harooni@scu.ac.ir
N
Ahmad Ali
Moazedi
Dept. of Biology, Faculty of Science, Shahid Chamran University of Ahwaz, Ahwaz, Iran.
moazedi_a@yahoo.cpm
N
Gholam Ali
Parham
Dept. of Statistic, Faculty of Mathematics, Shahid Chamran University of Ahwaz, Iran.
parham@scu.ac.ir
N
Somaye
Niknejad
Dept. of Biology, Faculty of Science, Shahid Chamran University of Ahwaz, Ahwaz, Iran.
nedalavadar@yahoo.com
N
Introduction: Nitric oxide is an important messenger in hippocampal region which is affected in learning and
memory processes and hippocampal responses to stress. In this study, we investigated the effect of L-NAME on passive
avoidance memory in adult male rats exposed to restraint-stress.
Methods: For this purpose, male Wistar rats were bilaterally implanted with cannulas aimed at the CA1 region of
the hippocampus. Thirty min after intrahippocampl injections of saline (alone) or L-NAME (20, 40 and
80μg/0.5μl/side) animals were immobilized in restrainer for 2 h/day for 7 days (7 days after surgery), and then were
tested for step-through latency and the time-spent in dark chamber in inhibitory avoidance task.
Results: Our findings showed that intrahippocampal injection of L-NAME (80 μg/0.5 μl/side) significantly
increased the step-through latency and decreased the time spent in the dark chamber in comparison with the control
group.
Conclusion: Since the inhibition of NO synthesis resulted in memory and learning improvements in rats exposed to
restraint-stress, NO might be involved in stress related learning and memory deficits.
Keywords: Nitric oxide, Passive avoidance,stress
http://ppj.phypha.ir/article-1-973-en.html
http://ppj.phypha.ir/article-1-973-en.pdf
Iranian Society of Physiology and Pharmacology
Physiology and Pharmacology
24765236
24765244
18
3
2014
10
1
Investigation of p53 and p27 expressions in the N-nitroso-N-methylureainduced breast cancer in female Wistar Albino rats
337
346
EN
Mahshid
Azizian
Dept. of Clinical Biochemistry, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran.
azizian424@yahoo.com
N
S. Zahra
Bathaie
Dept. of Clinical Biochemistry, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran.
bahai_z@modares.ac.ir
Y
Mahboobe
Ashrafi
Dept. of Basic Sciences, Faculty of Veterinary Medicine, Shiraz University, Shiraz, Iran.
mashrafi@shirazu.ac.ir
N
Reyhane
Hoshyar
Dept. of Biochemistry, Birjand University, Birjand, Iran
hooshyar@bums.ac.ir
N
Introduction: N-nitroso-N-methylurea (NMU) is a carcinogen from nitrosamines family, which has been used to
induce breast cancer in rodents. This model of breast cancer is very similar to the estrogen dependent breast cancer in
human. As a continuation of our recent works, in the present study, the expressions of both p53 and p27 were
investigated in NMU-induced breast cancer in Wistar Albino rats.
Methods: Breast cancer was induced by intraperitoneal injection of NMU in Wistar Albino rats, while, normal
saline was injected in the control group. After the tumors were formed and reached a proper size, the rats in both groups
were sacrificed under anesthesia and samples were obtained from mammary tumors as well as normal breast tissue.
Expressions of p53 and p27 genes were investigated by reverse-transcription polymerase chain reaction (RT- PCR).
Results: NMU injection induced breast cancer in rats. RT-PCR data showed that p53 expression was increased
(p>0.05), while p27 expression was decreased in rat mammary tumors compared with the normal tissue of normal rats
(p>0.05). This study is being continued to confirm the results, but these findings are compatible with our previous data
about cyclin D1 and p21 expressions in tumor tissue.
Conclusion: The results indicate opposite changes in the expression of p53 and p27 in cancerous breast tissue in
comparison with the normal breast tissue.
Breast Cancer, N-Nitroso-N-Methylurea (NMU),p53, p27
http://ppj.phypha.ir/article-1-1006-en.html
http://ppj.phypha.ir/article-1-1006-en.pdf
Iranian Society of Physiology and Pharmacology
Physiology and Pharmacology
24765236
24765244
18
3
2014
10
1
Effects of Citrullus Colocynthis pulp on serum testosterone and LH levels in streptozotocin-induced diabetic rats
347
353
EN
Fereshteh
Ostovan
Faculty of Science, Payamnor University, Iranshhar, Iran
fereshte.ostovan@yahoo.com
N
Ali
Gol
Dept. of Biology-Faculty of Science- Shahid Bahonar University of Kerman, Kerman, Iran
agol@mail.uk.ac.ir
Y
Hakimeh
Olomi
Dept. of Ecology, Institute of Science and High Technology and Environmental Sciences, Graduate University of Advanced Technology, Kerman, Iran
oloumi.ha@gmail.com
N
Introduction: Diabetes drastically increases the risk of developing reproductive system disorders. The aim of this
study was to investigate the effect of Citrullus Colocynthis pulp on testosterone and LH levels in streptozotocin-induced
diabetic rats.
Methods: Thirty-two male Wistar rats were divided into four groups as follows (n = 8 in each group): 1) normal, 2)
pulp, 3) diabetic, and 4) treatment groups. Normal and diabetic groups orally received 2 ml normal saline for 2 weeks,
while pulp and treatment groups orally received 30 mg/kg BW Citrullus Colocynthis pulp for 2 weeks. Diabetes was
induced by a single intraperitoneal injection of streptozotocin (65 mg/kg BW).
Results: The diabetic group showed a significant decrease in blood testosterone (p<0.001) and LH (P<0.01) levels
as compared to the normal group. However, treatment group had a significant increase (p<0.01) in testosterone and LH
levels as compared to the diabetic group.
Conclusion: The data suggested that Citrullus colocynthis pulp administered at 30 mg/kg BW for 2 weeks may have
beneficial effects on the damages in reproductive system, serum glucose impairment and loss of weight of testes caused
by diabetes in rats through hypothalamus– pituitary–testis axis.
Diabetes, Citrullus Colocynthis, testosterone, Luteinizing hormone, rat
http://ppj.phypha.ir/article-1-949-en.html
http://ppj.phypha.ir/article-1-949-en.pdf
Iranian Society of Physiology and Pharmacology
Physiology and Pharmacology
24765236
24765244
18
3
2014
10
1
Effects of mobile phone jammer on the anxiety level of male and female mice
354
363
EN
Majid
Jadidi
Research Center of Physiology, School of Medicine, Semnan University of Medical Sciences, Semnan, Iran
Jadidim@semums.ac.ir
N
Hossein
Miladi-Gorji
Research Center of Physiology, School of Medicine, Semnan University of Medical Sciences, Semnan, Iran
miladi331@yahoo.com
Y
Moghadaseh
Mahdinezhad
Dept. of Medical Physics, School of Medicine, Semnan University of Medical Sciences, Semnan, Iran
m.mohana6650@yahoo.com
N
Hojatollah
Torkmandi
Research Center of Physiology, School of Medicine, Semnan University of Medical Sciences, Semnan, Iran
m.mohana6650@yahoo.com
N
Introduction: With the increase in mobile phone usage in public places, the use of mobile phone jammer has also
increased therefore, the aim of this study was to evaluate the effects of mobile phone jammer on the level of anxiety in
mice.
Methods: Forty male and 40 female naive mice (25-30 g) were randomly divided into four groups (control, 900
MHz, 1800 MHz and 900+1800 MHz exposures). Animals were exposed to mobile phone jammer radiation for 2 h.
Then, anxiety-like behaviors were tested in the elevated plus-maze (EPM) model and light and dark (L/D) box.
Results: Results showed that mobile phone jammer exposure at 900 MHz reduced the time spent in the lit side of
the L/D box (P = 0.008) in male mice compared with the control group. While, 1800 MHz waves increased time spent
in the lit side of the L/D box and in the EPM open arm as compared to the control group in both male and female mice
(P = 0.05). Also, 1800 + 900 waves increased the time spent in the dark side of the L/D box in the female mice (P =
0.0001).
Conclusion: The results showed that mobile phone jammer radiation at 900 MHz and 900+1800 MHz frequencies
increased the level of anxiety in male and female mice, respectively. Thus, it seems that this apparatus cannot be used as
a safe device in popular places.
Mobile phone jammer, Elevated plus maze, Light and dark box, male and female mice
http://ppj.phypha.ir/article-1-1003-en.html
http://ppj.phypha.ir/article-1-1003-en.pdf
Iranian Society of Physiology and Pharmacology
Physiology and Pharmacology
24765236
24765244
18
3
2014
10
1
Cytotoxic effect of methanolic extracts of vegetative and reproductive parts of Vinca rosea on A431, a human skin squamous carcinoma cell line
364
372
EN
Yalda
Khazaei Poul
Dept. of Pharmacology, Shahid Beheshti University of Medical Sciences,Tehran, Iran
khazaei_yalda@yahoo.com
N
Ahmad
Majd
Dept. of Biology, Kharazmi University, Tehran, Iran
ahmad_majd2005@yahoo.com
N
Farzaneh
Labibi
Dept. of Immunology, Shahid Beheshti University of Medical Sciences, Tehran, Iran
labifar@yahoo.com
N
Taraneh
Moini Zanjani
Dept. of Pharmacology, Shahid Beheshti University of Medical Sciences,Tehran, Iran
tzanjani@yahoo.com
Y
Introduction: Vinca rosea, an important medicinal plant, is well-known for its anticancer properties especially
against breast cancer. In this study, we compared the cytotoxic effects of methanolic extracts of the vegetative (young
and old leaves) and reproductive (flowers) organs of this plant on a human squamous cell carcinoma using MTT assay.
Methods: The cytotoxic effects of Vinca rosea's methanolic extracts of young and old leaves as well as flowers
were assessed on A431, a human skin squamous carcinoma cell line using different concentrations. To compare the
cytotoxicities, cells without being exposed to extracts were used as control. Cells were incubated with plant extracts in
triplicate in 96 wells microplates and cytotoxicity was determined by the MTT assay. The experiments were repeated 4
times and IC50 values were calculated. Cell viability was also evaluated by trypan blue staining.
Results: A time dependent decrease in IC50 values of extracts was observed during the experimental period, and a
significant inhibition in cell proliferation was observed after the exposure of cells to the reproductive part of the plant
(flower) after 48 hours of incubation.
Conclusion: The results of this study showed that the reproductive part of the plant is more effective in reducing
cell proliferation compared to the vegetative organ. Moreover, the incubation time can influence the IC50 value and the
antiproliferative activity of the methanolic extract of Vinca rosea’s reproductive part.
Vinca rosea, methanolic extract, A431 cell line, MTT assay
http://ppj.phypha.ir/article-1-1010-en.html
http://ppj.phypha.ir/article-1-1010-en.pdf