2024-03-28T22:04:58+03:30 http://ppj.phypha.ir/browse.php?mag_id=73&slc_lang=en&sid=1
73-1310 2024-03-28 10.1002
Physiology and Pharmacology Physiol Pharmacol 24765236 24765244 10.61186/phypha 2018 22 1 Executive functions are related to serum testosterone and basal metabolism rate fluctuation but not lymphocyte dopamine receptor expression in the young healthy participants Mitra-Sadat Sadat-Shirazi mitrasadat@gamil.com Ghorbangol Ashabi as_habi@yahoo.com Nima Babhadiashar bobhadiashar@yahoo.com Mohammadreza Bahrami Hessari Bahrami@gmail.com Nasim Vousooghi dr.n.vosoughi@gmail.com Mohammad-Reza Zarrindast zarinmr@ams.ac.ir Introduction: Herein, we evaluated linkages between EFs performances and dopamine receptor (DR)  mRNA and testosterone level in the young Iranian male people. Methods: All 140 participants were normalized using depression, anxiety and stress scale questionnaire. Remained 108 volunteers were tested against drug abuse and then volunteers were distinguished by Wisconsin Card Sorting Test (WSCT). According to WCST, participants were divided into two low and high EFs performance. Afterward, anthropometric factors, body mass index (BMI) and serum testosterone level were measured in low and high EFs groups. Blood samples were collected, and biochemical and anthropometric data were evaluated; serum testosterone and DR mRNA expression were assessed in participants. Results: Data showed there are no differences between two groups in Na+, K+, glucose, urea, creatinine, SGPT, SGOT and other biochemical serum agents (P>0.05) but BMI was increased in low EFs compared with high EFs (P=0.000). Interestingly, there is no difference in DR expression between two groups (P>0.05). Conclusion: Our data presented that fluctuation of EFs performances in healthy adult male cases might depend on BMI and serum testosterone; while dopamine receptors in the blood lymphocytes had no substantial role in the EFs. High serum testosterone reduced EFs in the young adults. Executive function Dopamine receptor Testosterone Young people Blood lymphocytes Body mass index 2018 3 01 1 10 http://ppj.phypha.ir/article-1-1310-en.pdf
73-1339 2024-03-28 10.1002
Physiology and Pharmacology Physiol Pharmacol 24765236 24765244 10.61186/phypha 2018 22 1 Preventive effect of natural dietary supplement -Flavin7- on the onset of spontaneous diabetes mellitus in bio-breeding diabetes prone rats František Ništiar frantisek.nistiar@upjs.sk Agnesa Lukačínová agnesa.lukacinova1@upjs.sk Oliver Rácz oliver.racz@upjs.sk Jaroslava Nováková jaroslava.novakova@upjs.sk Eva Lovásová eva.lovasova@upjs.sk Marek Brenišin marek.brenisin@upjs.sk Simona Rusnáková simona.rusnakova@student.upjs.sk Introduction: The aim of the present study was to evaluate the preventive effects of Flavin7 in prediabetic bio-breeding diabetes prone (BB-DP) rats. Methods: Foutthy rats were divided into 2 equal groups: group C (untreated control group) and group F7 with Flavin7 (natural dietary supplement F7 with bioflavonoids, 0.2 mg/l) in drinking water from 21st day after birth to 171st day of their life, respectively. Blood glucose, superoxide dismutase, glutathione peroxidase, catalase, total antioxidant capacity, glutathione, body weight, food intake, water intake and urine output were determined.  Results: The age of diabetes onset was significantly higher for group F7 compared to group C (P<0.05). The incidence of diabetes was lower in group F7 than in group C. Blood glucose at the diabetes onset was higher in group C than in F7 group (P<0.05). Decrease of antioxidant status parameters, at the treatment onset as well as immediately after its termination showed a drop in the F7 group firstly, but increased progressively later, until the end of the experiment. Conclusion: F7 delayed the development of diabetes in BB-DP rats and prevented its onset. The severity of diabetes mellitus was milder in rats treated with F7 than in control group. Type 1 diabetes mellitus BB-DP rats Flavin7 Prevention of diabetes 2018 3 01 11 18 http://ppj.phypha.ir/article-1-1339-en.pdf
73-1329 2024-03-28 10.1002
Physiology and Pharmacology Physiol Pharmacol 24765236 24765244 10.61186/phypha 2018 22 1 Protective effects of rosuvastatin against hyperglycemia-induced oxidative damage in the pancreas of streptozotocin-induced diabetic rats Samira Yazdanimehr smehr461@yahoo.com Mohammad Taghi Mohammadi Mohammadimohammadt@bmsu.ac.ir Introduction: According to the powerful antioxidant effects of rosuvastatin, the present study aimed to examine the protective effects of rosuvastatin against oxidative damage of diabetic pancreas by potentiation of the antioxidant capacity in streptozotocin-induced diabetic rats. Methods: Experiment was performed in four groups of male Wistar rats (n=6 in each group): normal, diabetic and two treatment groups (normal and diabetic rats treated with rosuvastatin). Rats were made diabetic by a single intravenous injection of streptozotocin (40 mg/kg) at the beginning of study. Treatment groups received orally rosuvastatin at dose of 10 mg/kg/day. After eight weeks, the pancreas tissues were removed under deep anesthesia. After tissue homogenization, the contents of glutathione and malondialdehyde (MDA) as well as superoxide dismutase (SOD) activity were assessed by biochemical methods. Results: Blood glucose of diabetic rats was above 350 mg/dl. The MDA content of the homogenized pancreas significantly increased in diabetic rats by 92%. Diabetes also decreased the content of glutathione (32%) as well as SOD activity (68%) of pancreas tissues. Treatment with rosuvastatin noticeably decreased the MDA levels of diabetic pancreas (90%). Moreover, rosuvastatin significantly increased the glutathione content (21%) and SOD activity (67%) of pancreas tissues in treated diabetic rats. Conclusion: Our findings reveal that rosuvastatin is able to attenuate the uncontrolled hyperglycemia-induced oxidative damage of pancreas through potentiation of the antioxidant defense system. Diabetes mellitus Rosuvastatin Hyperglycemia Oxidative damage Antioxidant 2018 3 01 19 27 http://ppj.phypha.ir/article-1-1329-en.pdf
73-1294 2024-03-28 10.1002
Physiology and Pharmacology Physiol Pharmacol 24765236 24765244 10.61186/phypha 2018 22 1 The effect of saffron aqueous extract on oxidative stress parameters and important biochemical enzymes in the testis of streptozotocin-induced diabetic rats Mahmoud Hasanpour hasanpoor@shirazu.ac.ir Mahboobeh Ashrafi mashrafi@shirazu.ac.ir Hoda Erjaee hoda.erjaee@gmail.com Saeed Nazifi nazifi@shirazu.ac.ir Introduction: Sexual dysfunction and infertility are frequently associated with diabetes in men and experimental animals. Oxidative stress and alteration in testis are responsible for complication in diabetes. Saffron has antidiabetic and antioxidant properties that improves the functions of various organs. Therefore, the aim of the present study was to investigate the effects of administration of saffron aqueous extract in testis tissues of diabetic rats. Methods: The fasted rats were injected by a single intraperitoneal (ip) injection of a freshly prepared solution of streptozocin (STZ, 65mg/kg) in 0.1 M cold citrate buffer (pH=4.5). Three days after STZ administration, the animals with fasting blood glucose concentrations of over 250mg/dl were considered to be diabetic and were used in the experimental groups as follows: normal control (1), diabetic control (2), saffron control (3) and saffron treated (4). The treatment was started on the 7th day after STZ injection with ip injection of saffron (200mg/kg), five doses and weekly to groups (3 and 4). At the end of the experimental period, fasting blood glucose levels and the activity of ALT, AST, ALP, LDH, SOD, CAT, GPx and MDA content were determined in testis tissues. Results: Results showed saffron administration decreased elevated biochemical enzymes levels in testis of diabetic rats. Also, saffron significantly increased CAT and GPx activities in testis of diabetic rats. MDA levels had no significant changes in all experimental groups. Conclusion: The results demonstrated that saffron administration improved antioxidant enzymes function against oxidative stress. Saffron aqueous extract Diabetes mellitus Testis Biochemical and Antioxidant enzymes 2018 3 01 28 37 http://ppj.phypha.ir/article-1-1294-en.pdf
73-1266 2024-03-28 10.1002
Physiology and Pharmacology Physiol Pharmacol 24765236 24765244 10.61186/phypha 2018 22 1 Carbon nanotubes provide longer lasting gastroprotective effects for anandamide in stress-induced gastric ulcer in rat Parichehr Hassanzadeh p-hassanzadeh@razi.tums.ac.ir Elham Arbabi arbabiavalelham@gmail.com Fatemeh Atyabi atyabifa@tums.ac.ir Rassoul Dinarvand Dinarvand@tums.ac.ir Introduction: Anandamide (AEA) has shown a wide spectrum of pharmacological activities including the effects against the peptic ulcer, meanwhile, the poor solubility or short half-life may negatively affect the effectiveness of this valuable cannabinoid. Based on the superior properties of carbon nanotubes (CNTs) for controlled drug delivery, we aimed to prepare AEA-CNTs complex and evaluate its therapeutic potential in an experimental model of gastric ulcer. Methods: Amino-functionalized multi-walled CNTs-AEA (MWCNTs-AEA) complex was prepared using COOH-MWCNTs and then characterized by Fourier transform infrared spectroscopy and transmission electron microscopy. Gastric ulcer was induced by water immersion and restrain stress (WRS) for 3.5 and 6 h in rats and the gastric lesion and oxidative stress were evaluated. Results: AEA at higher doses reduced the gastric ulcer area and malondialdehyde content and elevated glutathione level and superoxide dismutase and catalase activities after 3.5-h WRS but it was ineffective after 6-h WRS. MWCNTs-AEA complex showed therapeutic effects after both 3.5- and 6-h WRS. Conclusion: Aminated MWCNTs are suitable carriers for AEA as they provide longer lasting effects for this cannabinoid. The antioxidant mechanism may be involved in the gastroprotective effects of MWCNTs-AEA complex. Carbon nanotubes Anandamide Gastric ulcer Rat 2018 3 01 38 47 http://ppj.phypha.ir/article-1-1266-en.pdf
73-1322 2024-03-28 10.1002
Physiology and Pharmacology Physiol Pharmacol 24765236 24765244 10.61186/phypha 2018 22 1 Gastroprotective effect of sodium hydrosulfide against indomethacin-induced gastric ulcer in diabetic rats Nasim Nazariani nazariani64@yahoo.com Seyyed Ali Mard alimard77@gmail.com; mard-sa@ajums.ac.ir Sima Nasri s_nasri2000@yahoo.com Ali Veisi Introduction: The incidence rate of gastric erosions and ulcers in diabetic patients are higher due to failure of mucosal antioxidant defense and maintain enough blood flow. The present study evaluated the gastro-protective effect of sodium hydrosulfide (NaHS) against indomethacin-induced gastric lesions in diabetic rats. Methods: In order to test anti-ulcer activity of NaHS against indomethacin, four diabetic groups of rats including diabetic control and 3 NaHS-treated groups received a single dose of physiologic saline or NaHS at 320, 640 and 1280 μg/kg respectively, 30 min before ulcer induction by indomethacin. Five hours later, the animals were killed and their stomachs were removed for macroscopically and microscopically evaluations. In order to evaluate the antacid effect of NaHS, 4 groups of diabetic rats received physiologic saline or NaHS at 320, 640 and 1280 μg/kg and 30 min later anesthetized, underwent a midline laparotomy and then their pylorus ligated. Five hours later, the animals were killed, their stomachs were removed and pH of gastric effluents were measured. Results: Indomethacin induced gastric lesions in glandular part of the stomach. NaHS at 640 and 1280 μg/kg significantly decreased the indomethacin-induced gastric lesions in diabetic rats. The pH of gastric effluents and mucus content increased by NaHS at doses of 640 and 1280 μg/kg. Macroscopic and microscopic observations showed that mucosal erosions induced by indomethacin were significantly inhibited by NaHS. Conclusion: results suggest NaHS through decreasing the rate of gastric acid output and increasing the mucus production, protected the gastric mucosa against indomethacin-induced gastric lesions in diabetic rats. Diabetes Gastric acid secretion sodium hydrosulfide Rat 2018 3 01 48 53 http://ppj.phypha.ir/article-1-1322-en.pdf
73-1305 2024-03-28 10.1002
Physiology and Pharmacology Physiol Pharmacol 24765236 24765244 10.61186/phypha 2018 22 1 The effect of carvacrol on transcription levels of Bcl-2 family proteins in hypertrophied heart of rats Sara Sadeghzadeh z.mohammadpour@stu.yazd.ac.ir Seyed Hasan Hejazian s.hejazian@ssu.ac.ir Mohabbat Jamhiri Zeynab Hafizibarjin hafizi.219@ssu.ac.ir Salman Sadeghzadeh salman.sdzd@iauashkezar.ac.ir Fatemeh Safari safarif@ssu.ac.ir Introduction: Cardiomyocytes apoptosis contributes to the development of left ventricular hypertrophy. The Bcl-2 family members are important regulators of mitochondrial pathway of apoptosis. Monoterpenoid phenol –carvacrol– possesses strong antioxidant properties. The present study aimed to evaluate the effect of carvacrol on transcription level of pro-apoptotic (Bad and Bax) and anti-apoptotic (Bcl-2 and BCL-xL) members of Bcl-2 family in hypertrophied hearts. Methods: Male Wistar rats (170-200 g) were divided into the following groups: (I) intact animals served as the control (Ctl), (II) un-treated rats subjected to aortic banding to induce left ventricular hypertrophy (H group), (III, IV, V and VI): carvacrol (C)-pretreated rats (5, 10, 25 and 50 mg/kg/day) subjected to aortic banding (H+C5, H+C10, H+C25 and H+C50 groups, respectively). Blood pressure was recorded through the carotid artery cannulation. Fibrosis was assessed by Masson’s trichrome staining. Gene expression was evaluated by real time-PCR technique. Results: In the H+C10, H+C25 and H+C50 groups mean arterial pressure (P<0.05, P<0.001 and P<0.001, respectively) and heart weight to body weight ratio (P<0.05, P<0.01 and P<0.001, respectively) were decreased significantly in comparison with H group. In the H group the Bad mRNA level was increased significantly compared to Ctl (P<0.001); while in the H+C10, H+C25 and H+C50 groups Bad mRNA level was decreased significantly (P<0.0 5, P<0.001 and P<0.001 vs. H). In H+C25 and H+C50 groups Bcl-2 and Bcl-xL mRNA were also up-regulated when compared with Ctl. Conclusion: Taken together, our results suggest that carvacrol may protect the hypertrophied heart against apoptosis by affecting transcription of Bcl-2 family members. Cardiac hypertrophy Apoptosis Carvacrol Bcl-2 Bax Bad Bcl-xl 2018 3 01 54 62 http://ppj.phypha.ir/article-1-1305-en.pdf
73-1336 2024-03-28 10.1002
Physiology and Pharmacology Physiol Pharmacol 24765236 24765244 10.61186/phypha 2018 22 1 Protective effects of omega-3, atorvastatin, vitamin E and vitamin C against doxorubicin-induced cardiotoxicity in rats: a comparison study Abbas Alimoradian alimoradian@arakmu.ac.ir Hadi Ansarihadipour Ansari@arakmu.ac.iro.com Saeed Changizi-Ashtiyani dr.ashtiyani@arakmu.ac.ir Ali Chehrei alichehrei@yahoo.com Reza Talebi rezatalebi29@gmail.com Sadaf Davudian sadaf.davoudian@humanitaresearch.it Soheila Rostami sadaf_davudian@yahoo.com Introduction: The stress-oxidative is involved in doxorubicin (DOX)-induced cardiotoxicity. Due to the potential and previous reported for antioxidant properties of atorvastatin, omega-3, vitamin E and vitamin C, their efficacy to prevention of DOX-induced cardiotoxicity was investigated in this study. Methods: Fifty-six male rats were divided into 8 groups which received omega-3, atorvastatin, vitamin E, vitamin C, normal saline and dimethyl sulfoxide (DMSO) via gavage for 14 days then a single dose of DOX (20 mg/kg) was injected intraperitoneally except two last groups that received only normal saline or DMSO. The level of oxidative stress parameters like ferric reducing ability of plasma (FRAP) before and after DOX injection and malondialdehyde (MDA) of heart were estimated. Also the histopathologic assessments were done on heart sample at the end of experimental period. Results: The results showed that compared to other agents, omega-3 could emerge as the most protection against DOX. Its pretreatment led to one of the most FRAP changing percent meanwhile less MDA value and cardio pathologic indexes almost close to control groups compared to that of other agents (P<0.01). Conclusion: Omega-3 may have a promising protective effect against DOX-induced cardio toxicity. Doxorubicin Omega-3 Vitamin E Vitamin C Atorvastatin Cardiotoxicity 2018 3 01 63 72 http://ppj.phypha.ir/article-1-1336-en.pdf