Purpose
The observation of DLBS1033 safety in terms of mutagenic using Ames and micronucleus tests.
Methods
Ames test was done using 3 strains of Salmonella typhimurium (TA98, TA100, and TA1535) with or without metabolic activation (S9 enzyme). In vitro micronucleus test was performed with prolonged exposure without S9. The variation of DLBS1033 concentrations were 100, 600, and 1200 µg/ml.
Results
The results of the Ames test (with/without S9) and in vitro micronucleus (prolonged exposure without S9) showed that DLBS1033 did not have a mutagenic effect.
Conclusion
DLBS1033 was safe and did not show any genotoxic activity.