Volume 29, Issue 2 (June 2025)                   Physiol Pharmacol 2025, 29(2): 205-218 | Back to browse issues page

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Shirazi tehrani A, Abbaszadeh H, Aliaghaei A, Nazarian H, Afshar A, Aghajanpour F, et al . Conditioned medium of Wharton’s jelly-derived mesenchymal stem cells restores spermatogenesis in scrotal hyperthermia-induced azoospermia. Physiol Pharmacol 2025; 29 (2) :205-218
URL: http://ppj.phypha.ir/article-1-2318-en.html
Conditioned medium of Wharton’s jelly-derived mesenchymal stem cells restores spermatogenesis in scrotal hyperthermia-induced azoospermia
Atefeh Shirazi tehrani , Hojjat-Allah Abbaszadeh , Abbas Aliaghaei , Hamid Nazarian , Azar Afshar , Fakhroddin Aghajanpour , Reza Soltani , Pourya Raee , Fatemeh Fadaei Fathabadi , Mohammad-Amin Abdollahifar
Abstract:   (1385 Views)

Introduction: Azoospermia is a common cause of male infertility. The latest hope for more affordable and safer therapies comes from stem cell research. In this study, the effect of mesenchymal stem cells derived from Wharton’s jelly on spermatogenesis in azoospermic mice with scrotal hyperthermia was investigated.
Methods: For the experimental study, 24 adult male mice were divided into four groups: 1. control (Cont), 2. scrotal hyperthermia (Hyp), 3. scrotal hyperthermia + DMEM (10μl) (Hyp/DMEM), 4. scrotal hyperthermia + conditioned medium (10 μl) (Hyp/CM). A temperature of 43 °C was applied for 20 minutes every other day for two weeks to induce hyperthermia. The mice in the experimental group received 10 µl of human Jelly Wharton’s Stem Cell-Conditioned Medium (hJWSC-CM) intraperitoneally every other day for 35 days after hyperthermia. Animals were sacrificed after the experiments to perform additional molecular, biochemical, and stereological analyses.
Results: The results showed that CM significantly increased both the total amount of sperm and the number of testicular cells, including spermatids, primary spermatocytes, spermatogonia, Leydig cells, and Sertoli cells, compared with Hyp and Hyp/DMEM; in addition, the biochemical characteristics of testicular tissue were significantly higher in the Hyp/CM group compared with the hyperthermia-induced groups. Further analysis revealed that when the Hyp/CM group was compared with the Hyp and Hyp/DMEM groups, Sox9 protein expression and proliferative gene expression increased significantly and the percentage of apoptotic testicular cells and pro-inflammatory gene expression decreased significantly.
Conclusion: These results suggest that realistic therapeutic approaches for reproductive and regenerative medicine could benefit from hWJSC-CM.

Keywords: Scrotal hyperthermia, Conditioned medium, Wharton's jelly, Spermatogenesis, Azoospermia
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Type of Manuscript: Experimental research article | Subject: Cell, Stem Cell and Cancer
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