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Abstract:   (2771 Views)
Introduction: Wound healing is one of the most critical issues human has been faced since the beginning of creation. Biodegradable polymers are of particular importance. In this study, cell free supernatant (CFS) of Bifidobacterium bifidum combined with chitosan (CS) film was evaluated as a wound dressing. Methods: Biodegradable films (CS and CFS/CS), as a novel wound dressing, were prepared. For evaluation of efficacy of the dressing, 45 male Wistar rats weighing 200-250 gr were randomly divided into 3 groups: negative control (without wound treatment), positive control (wound treatment by chitosan film), and probiotic (wound treatment by CFS/chitosan film). One full thickness wound was created on the dorsal area of the animals. The wound in positive control and probiotic groups were immediately covered by CS and CFS/CS dressing, respectively. Wound healing process and neo-tissue formation was evaluated by macroscopic observation, Histological analysis (H&E staining and Masson's trichrome staining) and gene expression (IL-1, TGF-β and IL-6) at 1, 3, 7, 14, and 21 post-operation days by RT-PCR method. Results: Our results showed different pattern of macrophages, fibroblasts and neutrophils infiltration in CFS/CH treated groups. Enhanced disposition of collagen and elastin caused improvement of wound healing process by the film. Base on the gene expression results, use of CFS/CH film caused improvement in wound healing kinetic. Conclusion: the biodegradable film based on chitosan and CFS of B. bifidum improves the wound healing process.
Keywords: Wound healing, Chitosan, Cell free supernatant, Biodegradable-film, Cytokines
     
Types of Manuscript: Experimental research article | Subject: Others

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