Volume 26, Issue 4 (December 2022)                   Physiol Pharmacol 2022, 26(4): 468-479 | Back to browse issues page

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Bazjou A, Jafari P, Marjani A, Akbari N. Effect of cell-free supernatant of Bifidobacterium bifidum combined with chitosan biodegradable film on full thickness wound healing in rats. Physiol Pharmacol 2022; 26 (4) :468-479
URL: http://ppj.phypha.ir/article-1-1657-en.html
Abstract:   (3521 Views)
Introduction: Wound healing is one of the most critical issues human has been faced since the beginning of creation. Biodegradable polymers are of particular importance. In this study, cell-free supernatant (CFS) of Bifidobacterium bifidum combined with chitosan (CH) film was evaluated as a wound dressing. Methods: Biodegradable films (CH and CFS/CH), as a novel wound dressing, were prepared. For the evaluation of dressing efficacy, 45 male Wistar rats weighing 200-250 g were randomly divided into 3 groups: negative control (without wound treatment), positive control (wound treatment by CH film), and probiotic (wound treatment by CFS/CH film). One full thickness wound was created on the dorsal area of the animals. The wound in positive control and probiotic groups were immediately covered by CH and CFS/CH dressing, respectively. Wound healing process was evaluated by macroscopic observation and histological analysis. During the treatment the expression of IL-1, TGF-B and IL-6 were assayed by qRT-PCR. Results: Our results showed different infiltration patterns of macrophages, fibroblasts, and neutrophils in CFS/CH treated group. Enhanced disposition of collagen and elastin caused improvement of wound healing process by the film. Based on the gene expression results, use of CFS/CH film caused improvement in wound healing kinetic. Conclusion: The biodegradable film based on chitosan and CFS of B. bifidum improves the wound healing process.
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