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Introduction: Metals such as silver have special merit in medicine. Recently, it has been shown that silver nanoparticles can present some properties that could be valuable for researchers because of their duel neuroprotective and neurotoxic behaviors. The present study was planned to consider the silver nanoparticles effectiveness on OLN-93 oligodendrocytes in chemical hypoxic situation. Methods: Our oligodendroglial cells model was OLN-93 cells. Chemical hypoxic- ischemic condition was induced by sodium azide (NaN3). After a three-hour pretreatment of OLN-93 cells with silver nanoparticles (0.001ppm), the cells were incubated in glucose-free medium with sodium azide (100mM and 1M) and silver nanoparticles (0.001ppm) for 15 minutes. Then, the reperfusion condition was set by returning the medium to DMEM with 10% FBS along with silver nanoparticles (0.001ppm) for 24 hours. Then, the cells were checked for viability by MTT assay and Transmission Electron Microscopy images. Results: Our results showed that silver nanoparticles with a concentration of 0.001ppm could significantly increase ONL-93 cells survival in hypoxic-ischemia induced by NaN3 (100mM; Mean ± SEM= 93.02 ± 2.83, P<0.001). However, Ag-NPs (0.001mM) could not protect the cells from hypoxic-ischemic injury induced by NaN3 (1M) through the same procedure. Conclusions: Although the neurotoxic effects of silver nanoparticles documented in many studies, silver nanoparticles solution with an average size of 40 nm and Ag ion concentration of 20.4 ppm could show protective effects on oligodendroglial cells in 0.001mM concentration. Hence, more investigation is suggested to clarify the protective effect of silver nanoparticles.
Types of Manuscript: Original Research | Subject: Cellular and Molecular BioMedicine

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