Volume 27, Issue 1 (March 2023)                   Physiol Pharmacol 2023, 27(1): 42-52 | Back to browse issues page

XML Print

Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:

Mostafavi H, Hatami M, Alipour M, Mousavi S S, Feizi H. Effect of cinnamon on antioxidant content and ZO-1 gene expression in the brain following middle cerebral artery occlusion in rats receiving high-fatdiet. Physiol Pharmacol 2023; 27 (1) :42-52
URL: http://ppj.phypha.ir/article-1-1823-en.html
Abstract:   (1411 Views)
Introduction: Despite all the research, no definitive treatment for stroke has been found yet. Cinnamon is a plant that has been shown to have health benefits effects. In this study, the effect of pretreatment of cinnamon on ischemia tolerance and the expression of Zonula occludens 1(ZO-1) gene in the brains of rats receiving a high-fat diet was investigated.
Methods: In this study, 72 rats were divided into six groups: control, sham, model (stroke), vehicle, lovastatin, and cinnamon. All groups except the control group received a high-fat diet for 8 weeks. Then the last three groups received Carboxymethyl cellulose, lovastatin, and cinnamon 130 mg accordingly for 6 weeks. Stroke was induced by middle cerebral artery occlusion (MCAO). Twelve hours later, the animals were examined for the extent of serum lipids, brain edema, anti-oxidant capacity and gene expression of ZO-1.
Results: Cinnamon was effective in reducing serum cholesterol and triglyceride. Cinnamon treatment significantly diminished brain edema. It also restored anti-oxidant capacity. ZO-1 gene expression was increased in the ischemic brains after cinnamon treatment (P< 0.05).
Conclusion: Pretreatment with Cin130 had beneficial effects on the serum lipid profile, edema volume in ischemic brain and anti-oxidant capacity. It increased ZO-1 gene expression and so maintained cellular integrity and prevented the subsequent edema.
Full-Text [PDF 4377 kb]   (252 Downloads)    
Type of Manuscript: Experimental research article | Subject: Others

1. Abdelnour, L. and F. El-Nagi (2017). Functional Neurological Disorder Presenting as Stroke: A Narrative Review. J Psychol Abnorm 6(159): 2.
2. Adibhatla R. M and H. J. F. (2008). Phospholipase A2, Reactive Oxygen Species, and Lipid Peroxidation In CNS pathologies. BMB Rep. 41(8): 560-567. [DOI:10.5483/BMBRep.2008.41.8.560]
3. Aebi, H. (1984). [13] Catalase in vitro. Methods in enzymology 105: 121-126. [DOI:10.1016/S0076-6879(84)05016-3]
4. Anderson, R. A., Z. Zhan, R. Luo, X. Guo, Q. Guo, J. Zhou, J. Kong, P. A. Davis and B. J. Stoecker (2016). Cinnamon extract lowers glucose, insulin and cholesterol in people with elevated serum glucose. J Tradit Complement Med 6(4): 332-336. [DOI:10.1016/j.jtcme.2015.03.005]
5. Andreadou, I., E. K. Iliodromitis, D. Farmakis and D. T. Kremastinos (2009). To prevent, protect and save the ischemic heart: antioxidants revisited. Expert Opin Ther Targets 13(8): 945-956. [DOI:10.1517/14728220903039698]
6. Babu, P. S., S. Prabuseenivasan and S. Ignacimuthu (2007). Cinnamaldehyde-a potential antidiabetic agent. Phytomedicine 14(1): 15-22. [DOI:10.1016/j.phymed.2006.11.005]
7. Beji, R. S., S. Khemir, W. A. Wannes, K. Ayari and R. Ksouri (2018). Antidiabetic, antihyperlipidemic and antioxidant influences of the spice cinnamon (Cinnamomum zeylanicumon) in experimental rats. Brazilian Journal of Pharmaceutical Sciences 54(2). [DOI:10.1590/s2175-97902018000217576]
8. Campbell, B. C. V., D. A. De Silva, M. R. Macleod, S. B. Coutts, L. H. Schwamm, S. M. Davis and G. A. Donnan (2019). Ischaemic stroke. Nat Rev Dis Primers 5(1): 70. 10.1038/s41572-019-0118-8
9. Chen, Y.-F., Y.-W. Wang, W.-S. Huang, M.-M. Lee, W. G. Wood, Y.-M. Leung and H.-Y. Tsai (2016). Trans-cinnamaldehyde, an essential oil in cinnamon powder, ameliorates cerebral ischemia-induced brain injury via inhibition of neuroinflammation through attenuation of iNOS, COX-2 expression and NFκ-B signaling pathway. Neuromolecular medicine 18(3): 322-333. [DOI:10.1007/s12017-016-8395-9]
10. Farhoudi M, Mehrvar K, Sadeghi-Bazargani H, Hashemilar M, Seyedi-Vafaee M, Sadeghi-Hokmabad E, Rikhtegar R, Saber-Maroof B, Abutalebi M, Rezaei M, Vaferi S, Aghili A and E. O (2017). Stroke subtypes, risk factors and mortality rate in northwest of Iran. Iran J Neurol. 16(3): 112-117.
11. Frydman-Marom, A., A. Levin, D. Farfara, T. Benromano, R. Scherzer-Attali, S. Peled, R. Vassar, D. Segal, E. Gazit, D. Frenkel and M. Ovadia (2011). Orally Administrated Cinnamon Extract Reduces β-Amyloid Oligomerization and Corrects Cognitive Impairment in Alzheimer's Disease Animal Models. PLoS One 6(1). [DOI:10.1371/journal.pone.0016564]
12. G Y Yang and A L Betz (1994). Reperfusion-Induced Injury to the Blood-Brain Barrier After Middle Cerebral Artery Occlusion in Rats. Stroke 25(8): 1658-1664. [DOI:10.1161/01.STR.25.8.1658]
13. Ghosh T, Basu A, Adhikari D, Roy D and Pal A.K (2015). Antioxidant activity and structural features of Cinnamomum zeylanicum. 3 Biotech. Dec; 5(6): 939-947. [DOI:10.1007/s13205-015-0296-3]
14. Giustarini, D., I. Dalle-Donne, D. Tsikas and R. Rossi (2009). Oxidative stress and human diseases: Origin, link, measurement, mechanisms, and biomarkers. Crit Rev Clin Lab Sci 46(5-6): 241-281. [DOI:10.3109/10408360903142326]
15. Hosseini A A, Sobhani-Rad D, Ghandehari K and Benamer H (2010). Frequency and clinical patterns of stroke in Iran - Systematic and critical review. BMC Neurol 10(72). [DOI:10.1186/1471-2377-10-72]
16. Ighodaro, O. M. and O. A. Akinloye (2018). First line defence antioxidants-superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GPX): Their fundamental role in the entire antioxidant defence grid. Alexandria Journal of Medicine 54(4): 287-293. [DOI:10.1016/j.ajme.2017.09.001]
17. Jana, A., K. K. Modi, A. Roy, J. A. Anderson, R. B. van Breemen and K. Pahan (2013). Up-regulation of neurotrophic factors by cinnamon and its metabolite sodium benzoate: Therapeutic implications for neurodegenerative disorders. J Neuroimmune Pharmacol 8(3): 739-755. [DOI:10.1007/s11481-013-9447-7]
18. Jiao, H., Z. Wang, Y. Liu, P. Wang and Y. Xue (2011). Specific role of tight junction proteins claudin-5, occludin, and ZO-1 of the blood-brain barrier in a focal cerebral ischemic insult. J Mol Neurosci 44(2): 130-139. [DOI:10.1007/s12031-011-9496-4]
19. Kalogeris T, Baines CP, Krenz M and Korthuis RJ (2016). Ischemia/Reperfusion. Compr Physiol. 6(7(1)): 113-170. [DOI:10.1002/cphy.c160006]
20. Kalogeris, T., Y. Bao and R. J. Korthuis (2014).Mitochondrial reactive oxygen species: a double edged sword in ischemia/reperfusion vs preconditioning. Redox Biol 2: 702-714. [DOI:10.1016/j.redox.2014.05.006]
21. Lewis A and S. A. (2010). Hyperlipidemia and primary prevention of stroke: does risk factor identification and reduction really work? Curr Atheroscler Rep. Jul;12(4): 225-229. [DOI:10.1007/s11883-010-0117-4]
22. Longa EZ, Weinstein PR, Carlson S and Cummins R (1989). Reversible middle cerebral artery occlusion without craniectomy in rats. Stroke 20: 84-91. [DOI:10.1161/01.STR.20.1.84]
23. Luissint, A.-C., C. Artus, F. Glacial, K. Ganeshamoorthy, P.-O. J. F. Couraud and B. o. t. CNS (2012). Tight junctions at the blood brain barrier: physiological architecture and disease-associated dysregulation. Fluids Barriers CNS. 9(1): 23. [DOI:10.1186/2045-8118-9-23]
24. Marques C, Meireles M, Norberto S, Leite J, Freitas J, Pestana D, Faria A and C. C. (2015). High-fat diet-induced obesity Rat model: a comparison between Wistar and Sprague-Dawley Rat. Adipocyte. Jul 15(5(1)): 11-21. [DOI:10.1080/21623945.2015.1061723]
25. McCord, J. M. and I. Fridovich (1969). Superoxide dismutase: an enzymic function for erythrocuprein (hemocuprein). Journal of Biological chemistry 244(22): 6049-6055. [DOI:10.1016/S0021-9258(18)63504-5]
26. Mirhadi, K. (2011). Effect of Intraperitoneally Injection of Different Doses of Lovastatin on Pain and Inflammatory Response Induced by Formalin in Mice American Journal of Animal and Veterinary Sciences 6(4): 160-165. [DOI:10.3844/ajavsp.2011.160.165]
27. Moradi, P., M. Ganjkhani, I. J. Anarkooli and A. Abdanipour (2019). Neuroprotective effects of lovastatin in the pilocarpine rat model of epilepsy according to the expression of neurotrophic factors. Metabolic brain disease 34(4): 1061-1069. [DOI:10.1007/s11011-019-00424-1]
28. Nelson., R. H. ( 2013). Hyperlipidemia as a Risk Factor for Cardiovascular Disease. Prim Care. Mar; 40(1): 195-211. [DOI:10.1016/j.pop.2012.11.003]
29. Panahpour, H., A. A. Nekooeian and G. A. Dehghani (2014). Candesartan attenuates ischemic brain edema and protects the blood-brain barrier integrity from ischemia/reperfusion injury in rats. Iranian biomedical journal 18(4): 232.
30. Panickar, K. S. and R. A. Anderson (2011). Effect of polyphenols on oxidative stress and mitochondrial dysfunction in neuronal death and brain edema in cerebral ischemia. Int J Mol Sci 12(11): 8181-8207. [DOI:10.3390/ijms12118181]
31. R Buettner, K G Parhofer, M Woenckhaus, C E Wrede, L A Kunz-Schughart, J Schölmerich and L. C. Bollheimer (2006). Defining high-fat-diet rat models: metabolic and molecular effects of different fat types. J Mol Endocrinol 2006 Jun;36(3):485-501. [DOI:10.1677/jme.1.01909]
32. Ranasinghe, P., S. Perera, M. Gunatilake, E. Abeywardene, N. Gunapala, S. Premakumara, K. Perera, D. Lokuhetty and P. Katulanda (2012). Effects of Cinnamomum zeylanicum (Ceylon cinnamon) on blood glucose and lipids in a diabetic and healthy rat model. Pharmacognosy Res 4(2): 73-79. [DOI:10.4103/0974-8490.94719]
33. Rao PV and G. SH. (2014). Cinnamon: a multifaceted medicinal plant. Evidence-Based Complementary and Alternative Medicine 2014: 642942. [DOI:10.1155/2014/642942]
34. Rempe R. G, Hartz A. MS and B. B (2016). Matrix metalloproteinases in the brain and blood-brain barrier: Versatile breakers and makers. J Cereb Blood Flow Metab. 36(9): 1481-1507. [DOI:10.1177/0271678X16655551]
35. Sedighi M, Bahmani M, Asgary S, Beyranvand F and R.-K. M. (2017). A review of plant based compounds and medicinal plants effective on atherosclerosis. J Res Med Sci 22(30). [DOI:10.4103/1735-1995.202151]
36. Stavinoha R. C and D. A. Vattem (2015). Potential neuroprotective effects of cinnamon International Journal of Applied Research in Natural Products 8(3): 24-46.
37. Sun M-Sh, Jin H, Sun X, Huang Sh, Zhang Fu-L, Guo Zh-Ni and Yang Y (2018). Free Radical Damage in Ischemia-Reperfusion Injury: An Obstacle in Acute Ischemic Stroke after Revascularization Therapy. Oxidative Medicine and Cellular Longevity: 17 pages. [DOI:10.1155/2018/3804979]
38. Tan, B. L. and M. E. Norhaizan (2019). Effect of High-Fat Diets on Oxidative Stress, Cellular Inflammatory Response and Cognitive Function. Nutrients 11(11): 2579. [DOI:10.3390/nu11112579]
39. Tuzcu Z, Orhan C, Sahin N, Juturu V and Sahin K (2017). Cinnamon Polyphenol Extract Inhibits Hyperlipidemia and Inflammation by Modulation of Transcription Factors in High-Fat Diet-Fed Rats. Oxidative Medicine and Cellular Longevity 2017: 10 pages. [DOI:10.1155/2017/1583098]
40. Vafa, M., F. Mohammadi, F. Shidfar, M. S. Sormaghi, I. Heidari, B. Golestan and F. Amiri (2012). Effects of cinnamon consumption on glycemic status, lipid profile and body composition in type 2 diabetic patients. Int J Prev Med 3(8): 531-536.
41. Verspohl EJ, Bauer K and N. E. (2005). Antidiabetic effect of Cinnamomum cassia and Cinnamomum zeylanicum in vivo and in vitro. Phytother Res. Mar;19(3): 203-206. [DOI:10.1002/ptr.1643]
42. Xue, R., J. Lv, J. Gao, R. Fu, W. Li, X. Lei, G. Wu, L. Xue and Z. Zhang (2013). Protective effect of tea polyphenols on the blood-brain barrier. Translational Neuroscience 4(3): 295-301. [DOI:10.2478/s13380-013-0133-2]
43. Yulug, B., E. Kilic, S. Altunay, C. Ersavas, C. Orhan, A. Dalay, M. Tuzcu, N. Sahin, V. Juturu and K. Sahin (2018). Cinnamon Polyphenol Extract Exerts Neuroprotective Activity in Traumatic Brain Injury in Male Mice. CNS Neurol Disord Drug Targets 17(6): 439-447. [DOI:10.2174/1871527317666180501110918]
44. Zeljkovic, A., J. Vekic, V. Spasojevic-Kalimanovska, Z. Jelic-Ivanovic, N. Bogavac-Stanojevic, B. Gulan and S. Spasic (2010). LDL and HDL subclasses in acute ischemic stroke: prediction of risk and short-term mortality. Atherosclerosis 210(2): 548-554. [DOI:10.1016/j.atherosclerosis.2009.11.040]

Rights and permissions
Creative Commons License This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.