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Introduction: Oleogumresins extracted from Boswellia sacra (Frankincense) and Commiphora myrrha (Myrrha) are used to heal wounds and skin injuries. Moreover, they have anti-inflammatory, antioxidant, and antimicrobial effects. Therefore, we hypothesized that their combination can be effective in wound healing. Herein, the effects of the methanol extract of two oleogumresins namely Boswellia sacra (Frankincense) and Commiphora myrrha (Myrrha) and their combination on cell migration and wound healing process were evaluated in human dermal fibroblast cells (HDFa). 
Methods: The methanol extracts of B. sacra (BS) and C. myrrha (CM) and their combination tested to obtain their optimum cytoprotective concentrations using AlamarBlue assay. The level of reactive oxygen species (ROS) was also evaluated using a DCFDA detector. To assess the cell migration promoting and wound healing properties of the extracts, a scratch wound closure assay was performed in HDFa cells and the images were analyzed using ImageJ software. Western blot analysis was used to detect the activation of fibroblast migration-associated protein ERK.  
Results: Using the viability assay, the optimum non-cytotoxic concentrations of the extracts (10 and 20 µg/ml) were chosen to evaluate their wound healing activities on HDFa cells. BS, CM and BC at 10 and 20 µg/ml were able to significantly reduce H2O2-induced ROS levels compared to the control. In scratch assay, BS and BC, both at 10 µg/ml, could significantly reduce the average wound width compared to the control. Western blotting analysis showed that CM significantly increased the pERK/ERK ratio compared to the control.

Types of Manuscript: Experimental research article | Subject: Others

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