Glucocorticoids are widely used to treat various disorders, but their administration is associated with multiple unwanted effects. Currently, many of these side effects are considered to develop pre-clinical animal models. Experimental models that reproduce crucial features of the human diseases can provide the useful approaches to examine the potential therapeutic agents and to elucidate the molecular and cellular pathways in a regulated environment. In this review, various animal models in which glucocorticoids have been used for induction of human disorders in different body systems including hypertension, skin atrophy, hair loss, insulin resistance, dyslipidemia, gastric mucosal damage, growth retardation, muscle atrophy, osteoporosis, osteonecrosis, depression-like behavior, glaucoma and cataracts were summarized.