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High concentration of nitric oxide impaired proliferation of bone marrow mesenchymal stem cells due to cell cycle arrest at G1 stage
Mahsa Maleklou , Mohammad Husein Abnosi
Abstract:   (379 Views)
Although exogenous nitric oxide (NO) is used as medicine, in the previous we showed its inhibitory effect of on proliferation ability of rat bone marrow mesenchymal stem cells (BMSCs). In present investigation, the inhibitory role of exogenous NO on BMSCs cell cycle was studied. BMSCs after third passage were treated for one hour in every 48 hours with 100μM of sodium nitroprusside as NO donor. Then after 5,10,15, and 20 days of treatment, the viability, proliferation and cell cycle of the BMSCs was investigated. In addition, the expression of the Raf1, CDK2, CDK4, P53, and GAPDH genes was studied. Cells treatment caused significant reduction of viability and proliferation at 5,10,15, and 20 days. Also, the treatment caused cell cycle arrest at G1 after 20 days. In addition, it was found that the CDK2 and CDK4 expression was down-regulated whereas the P53 expression was up-regulated, but the expression of Raf1 as well as GAPDH remained the same. This study showed, prolong treatment with NO donor arrest the BMSCs cell cycle due to over expression of P53 which inhibit the expression of Cdk2 and Cdk4.
Keywords: Cell cycle, Nitric oxide, Mesenchymal stem cells, cyclin-dependent kinases, Cell proliferation
     
Type of Manuscript: Experimental research article | Subject: Cell, Stem Cell and Cancer
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