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Chrysin potential on the amelioration of Cyclophosphamide toxic effects on mouse oocytes and embryos
Sima Etebari 
, Mojdeh Gholami , Mohsen Asouri 
, Mehrab Nasiri kenari , Yahya Babaki , Mohammad Reza Momtaz , Ali Asghar Ahmadi
, Mojdeh Gholami , Mohsen Asouri , Mehrab Nasiri kenari , Yahya Babaki , Mohammad Reza Momtaz , Ali Asghar Ahmadi
Abstract: (86 Views)
Objective: The study investigates the protective effects of chrysin (CR) on the quality of oocytes and embryos of in vitro fertilization in rats treated with cyclophosphamide (CPH).
Materials and Methods: This study, female NMRI mice were divided into five groups: I. control group, II. sham group, III. CPH group (receiving 120 mg/kg.wk of CPH intraperitoneally (IP), IV and V: CR groups receiving 5 and 10 mg/kg. day CR for four weeks. For oocyte induction, ten units of pregnant mare serum gonadotropin was injected intraperitoneally after the last injection. All mice were then sacrificed by aspiration of their oocytes for further experiments. The growth of embryos was investigated using mature oocytes in vitro.
Results: CR significantly increased the number of 2 cells and 4 cells after 24 and 48 hours compared to the CPH group. Groups treated with CR showed a significant increase in the expression level of BMP-15 and GDF-9 gene in a dose-dependent manner compared to the CPH group.
Conclusion: In mice, CR reduced oxidative damage and oocyte cytokine levels in ovarian tissue after CPH-induced degeneration.
Materials and Methods: This study, female NMRI mice were divided into five groups: I. control group, II. sham group, III. CPH group (receiving 120 mg/kg.wk of CPH intraperitoneally (IP), IV and V: CR groups receiving 5 and 10 mg/kg. day CR for four weeks. For oocyte induction, ten units of pregnant mare serum gonadotropin was injected intraperitoneally after the last injection. All mice were then sacrificed by aspiration of their oocytes for further experiments. The growth of embryos was investigated using mature oocytes in vitro.
Results: CR significantly increased the number of 2 cells and 4 cells after 24 and 48 hours compared to the CPH group. Groups treated with CR showed a significant increase in the expression level of BMP-15 and GDF-9 gene in a dose-dependent manner compared to the CPH group.
Conclusion: In mice, CR reduced oxidative damage and oocyte cytokine levels in ovarian tissue after CPH-induced degeneration.
Type of Manuscript: Experimental research article |
Subject:
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