Volume 28, Issue 4 (December 2024)
Physiol Pharmacol 2024, 28(4): 389-399 |
Back to browse issues page
Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:
Soltanbeigi S, Fahimi Z, Maleki M, Mozafari A, Kaffashian M R. The efficacy of Hypericum helianthemoides
extraction with phenytoin in diabetic and non-diabetic wound healing: An experimental study in
rats. Physiol Pharmacol 2024; 28 (4) : 2
URL: http://ppj.phypha.ir/article-1-2340-en.html
URL: http://ppj.phypha.ir/article-1-2340-en.html
Abstract: (962 Views)
Introduction: Traditional medicine recommends herbal medicines for metabolic disorders. The present study explores the skin wound healing potential of Hypericum helianthemoides (H. helianthemoides) extract in diabetic and non-diabetic rats.
Methods: Following wound induction in diabetic (n=50) (induced by a single dose of streptozotocin) and non-diabetic (n=50) rats, H. helianthemoides extract (5% and 10%) was administered versus standard drug phenytoin (1%) and Osrin to positive and sham control groups. Tropical ointment therapy was applied once a day until the end of the study period (20 days). A Vernier caliper (with a 0.1 mm accuracy) was used to measure the wound length at 1, 3, 7, 11, 15, and 20 days after induction. Furthermore, pathological examination categorized the wound healing process into five categories: poor, mild, moderate, fair, and excellent.
Results: On the study’s first day, both diabetic and nondiabetic rats had the same wound area size. After 11 days, the wound area size significantly decreased in groups treated with 5% and 10% H. helianthemoides extract compared to the sham and control groups (P<0.001). Hence, based on the wound pathological evaluation scale, the most frequent phenytoin and H. helianthemoides extract-treated groups were classified as moderate to excellent (P<0.05).
Conclusion: H. helianthemoides extract accelerates full-thickness wound healing in diabetic and non-diabetic rats
Methods: Following wound induction in diabetic (n=50) (induced by a single dose of streptozotocin) and non-diabetic (n=50) rats, H. helianthemoides extract (5% and 10%) was administered versus standard drug phenytoin (1%) and Osrin to positive and sham control groups. Tropical ointment therapy was applied once a day until the end of the study period (20 days). A Vernier caliper (with a 0.1 mm accuracy) was used to measure the wound length at 1, 3, 7, 11, 15, and 20 days after induction. Furthermore, pathological examination categorized the wound healing process into five categories: poor, mild, moderate, fair, and excellent.
Results: On the study’s first day, both diabetic and nondiabetic rats had the same wound area size. After 11 days, the wound area size significantly decreased in groups treated with 5% and 10% H. helianthemoides extract compared to the sham and control groups (P<0.001). Hence, based on the wound pathological evaluation scale, the most frequent phenytoin and H. helianthemoides extract-treated groups were classified as moderate to excellent (P<0.05).
Conclusion: H. helianthemoides extract accelerates full-thickness wound healing in diabetic and non-diabetic rats
Article number: 2
Keywords: Hypericum helianthemoides, Wound healing, Herbal medicine, Streptozotocin, Diabetes mellitus
Type of Manuscript: Experimental research article |
Subject:
Clinical Physiology/Pharmacology
References
1. Altan A, Aras M H, Damlar İ, Gökçe H, Özcan O, Alpaslan C. The effect of Hypericum Perforatum on wound healing of oral mucosa in diabetic rats. Eur Oral Res 2018; 52: 143-149. [DOI:10.26650/eor.2018.505]
2. American Diabetes A. Diagnosis and classification of diabetes mellitus. Diabetes Care 2013; 37: S81-S90. [DOI:10.2337/dc14-S081]
3. Anstead G M, Hart L M, Sunahara J F, Liter M E. Phenytoin in wound healing. Ann Pharmacother 1996; 30: 768-775. [DOI:10.1177/106002809603000712]
4. Ashrafi A, Rezaii A A, Haghdost I S, Mehdineghad B, Ashrafi I, Asmariyan S H. Histometric and histophatologic evaluation of the effects of equistum arvense herbal extract versus zinc oxide in rabbit skin wound healing model. Veterinary Medicine Faculty 2010; 4: 843-853.
5. Attinger C E, Janis J E, Steinberg J, Schwartz J, Al-Attar A, Couch K. Clinical approach to wounds: débridement and wound bed preparation including the use of dressings and wound-healing adjuvants. Plast Reconstr Surg 2006; 117: 72s-109s. [DOI:10.1097/01.prs.0000225470.42514.8f]
6. Bedi M K, Shenefelt P D. Herbal therapy in dermatology. Arch Dermatol 2002; 138: 232-242. [DOI:10.1001/archderm.138.2.232]
7. Bhatia A, Prakash S. Topical phenytoin for wound healing. Dermatol Online J 2004; 10: 5. [DOI:10.5070/D30Z3612W1]
8. Boissier E. Flora orientalis, sive enumeratio plantarum in Oriente a Graecia et Aegypto ad Indiae fines hucusque observatarum. Georg; 1879. [DOI:10.5962/bhl.title.20323]
9. Bork P M, Bacher S, Schmitz M L, Kaspers U, Heinrich M. Hypericin as a non-antioxidant inhibitor of NF-kappa B. Planta Med 1999; 65: 297-300. [DOI:10.1055/s-1999-13989]
10. Bowers S, Franco E. Chronic Wounds: Evaluation and Management. Am Fam Physician 2020; 101: 159-166.
11. Brondz I, Greibrokk T, Groth P A, Aasen A J. The relative stereochemistry of hyperforin - an antibiotic from hypericum perforatum L. Tetrahedron Letters 1982; 23: 1299-1300. [DOI:10.1016/S0040-4039(00)87088-4]
12. Cañedo-Dorantes L, Cañedo-Ayala M. Skin acute wound healing: A comprehensive review. Int J Inflam 2019; 2019: 3706315. [DOI:10.1155/2019/3706315]
13. Chakraborty R, Borah P, Dutta P P, Sen S. Evolving spectrum of diabetic wound: Mechanistic insights and therapeutic targets. World J Diabetes 2022; 13: 696-716. [DOI:10.4239/wjd.v13.i9.696]
14. Cinci L, Di Cesare Mannelli L, Maidecchi A, Mattoli L, Ghelardini C. Effects of Hypericum perforatum extract on oxaliplatin-induced neurotoxicity: in vitro evaluations. Z Naturforsch C J Biosci 2017; 72: 219-226. [DOI:10.1515/znc-2016-0194]
15. Dorsett-Martin W A. Rat models of skin wound healing: a review. Wound Repair Regen 2004; 12: 591-599. [DOI:10.1111/j.1067-1927.2004.12601.x]
16. Farsak M, Özdağli G, Özmüş D, Çömelekoğlu Ü, Yalın S, Bozdoğan Arpacı R, et al. Effects of hypericum perforatum on an experimentally induced diabetic wound in a rat model. Wounds 2017; 29: 10-17.
17. Fernández-Guarino M, Hernández-Bule M L, Bacci S. Cellular and molecular processes in wound healing. Biomedicines 2023; 11: 2526. [DOI:10.3390/biomedicines11092526]
18. Gangemi S, Minciullo P L, Miroddi M, Chinou I, Calapai G, Schmidt R J. Contact dermatitis as an adverse reaction to some topically used European herbal medicinal products - part 2: Echinacea purpurea-Lavandula angustifolia. Contact Dermatitis 2015; 72: 193-205. [DOI:10.1111/cod.12328]
19. Graves L E, Donaghue K C. Vascular complication in adolescents with diabetes mellitus. Frontiers in Endocrinology 2020; 11. [DOI:10.3389/fendo.2020.00370]
20. Gruenwald J. B T. PDR for herbal medicines. Rutgers: Fourth edition. Montvale, NJ : Thomson, [2007] ©2007, 2007.
21. Haji Zadeh S, Khoshbaten A L I, Asgari A, Khaksari M. Low voltage electrical stimulation and wound healing in rabbits: effect of altering frequency. MJIRI 1996; 10: 225-228.
22. Hammer K D, Hillwig M L, Solco A K, Dixon P M, Delate K, Murphy P A, et al. Inhibition of prostaglandin E(2) production by anti-inflammatory hypericum perforatum extracts and constituents in RAW264.7 Mouse Macrophage Cells. J Agric Food Chem 2007; 55: 7323-7331. [DOI:10.1021/jf0710074]
23. Heydari M, Poorbabaei H, Esmaaelzade O. Indicator plant species in monitoring forest soil conditions using logistic regression model in Zagros Oak (Quercus brantii var.persica) forest ecosystems, Ilam city. Journal of Plant Research (Iranian Journal of Biology) 2015; 27: 811-828.
24. Hoffmann J, Gendrisch F, Schempp C M, Wölfle U. New herbal biomedicines for the topical treatment of dermatological disorders. Biomedicines 2020; 8. [DOI:10.3390/biomedicines8020027]
25. Jafari Barmak M. Compare Oak extract, Teucrium Polium, Hyper Perforatum and silver sulfadiazine1% on a second grade burn wounds in rats. Nautilus 2014; 128: 28-32.
26. Janis J. Clinical approach to wounds: debridement and wound bed preparation including the use of dressings and wound-healing adjuvants. American Society of Plastic Surgeons 2006; 117. [DOI:10.1097/01.prs.0000225470.42514.8f]
27. Jeffcoate W J, Harding K G. Diabetic foot ulcers. Lancet 2003; 361: 1545-1551. [DOI:10.1016/S0140-6736(03)13169-8]
28. khaksar s, kesmati m, rezaie a, Rasekh A. Topical estrogen accelerates wound healing in diabetic rats. IJEM 2011; 12: 544-551.
29. Kucukboyaci N, Tamer U, Karasu Ç, Torul H. Evaluation of phenolic compounds and protective effects of olive (Olea europaea L.) leaf extracts on endothelial cells against hydrogen peroxide-induced toxicity. Journal of Research in Pharmacy 2020; 24: 497-507. [DOI:10.35333/jrp.2020.198]
30. LoGerfo F W, Coffman J D. Vascular and microvascular disease of the foot in diabetes. New England Journal of Medicine 1984; 311: 1615-1619. [DOI:10.1056/NEJM198412203112506]
31. M. Mahmoudi M, Javanmardi A, Morteza Semnani K, Saeedi M. Anti-inflammatory, analgesic activity, acute toxicity and hypericins content of iranian hypericum perforatum. Babol-Jbums 2006; 8: 7-14.
32. Menegazzi M, Di Paola R, Mazzon E, Muià C, Genovese T, Crisafulli C, et al. Hypericum perforatum attenuates the development of carrageenan-induced lung injury in mice. Free Radic Biol Med 2006; 40: 740-753. [DOI:10.1016/j.freeradbiomed.2005.08.034]
33. Mirzaei M, Sewell R D E, Kheiri S, Rafieian-kopaei M. A clinical trial of the effect of St. John’s wort on migraine headaches in patients receiving sodium valproate. Journal of Medicinal Plants Research 2012; 6: 1524-1531. [DOI:10.5897/JMPR11.431]
34. Momeni E, Aroi N, Aroi M, Jafari Barmak M, Mahmoudi R, Malekzadeh J M, et al. the effect of hypericum perforatum extract and 1 % silver sulfadiazine on second degree burn wound healing in male rats. yums-armaghan 2014; 19: 625-632.
35. Nikrooze L, Jafari Barmak M, Naghmachi M, Ghafarian shirazi H, Dehghani N. Study of Jaft Aqueous Extract and Silver Sulfadiazine on Burn Healing in Male Rat. yums-armaghan 2013; 18: 107-114.
36. Novelli M, Masiello P, Beffy P, Menegazzi M. Protective role of St. John’s Wort and its components hyperforin and hypericin against diabetes through inhibition of inflammatory signaling: evidence from in vitro and in vivo studies. Int J Mol Sci 2020; 21. [DOI:10.3390/ijms21218108]
37. Oryan A, Naeini A, B N, Gorjlan E. Effect of aqueous extract of Aloe vera on experimental cutaneous wound healing in rat. Veterinarski Arhiv 2010; 80: 509-522.
38. Prisăcaru A I, Andriţoiu C V, Andriescu C, Hăvârneanu E C, Popa M, Motoc A G, et al. Evaluation of the wound-healing effect of a novel Hypericum perforatum ointment in skin injury. Rom J Morphol Embryol 2013; 54: 1053-9.
39. Punjataewakupt A, Napavichayanun S, Aramwit P. The downside of antimicrobial agents for wound healing. Eur J Clin Microbiol Infect Dis 2019; 38: 39-54. [DOI:10.1007/s10096-018-3393-5]
40. Ramezani J, Azarbayjani M A, Peeri M. Simultaneous effects of aerobic training and berberine chloride on plasma glucose, IL-6 and TNF-α in type 1 diabetic male wistar rats. Nutrition and Food Sciences Research 2019; 6: 9-16. [DOI:10.29252/nfsr.6.1.9]
41. Robertson S, Cameron N E, Cotter M A. The effect of the calcium antagonist nifedipine on peripheral nerve function in streptozotocin-diabetic rats. Diabetologia 1992; 35: 1113-1117. [DOI:10.1007/BF00401363]
42. Rodríguez-Rodríguez N, Martínez-Jiménez I, García-Ojalvo A, Mendoza-Mari Y, Guillén-Nieto G, Armstrong D G, et al. Wound chronicity, impaired immunity and infection in diabetic patients. MEDICC Rev 2022; 24: 44-58. [DOI:10.37757/MR2021.V23.N3.8]
43. Sánchez-Mateo C C, Bonkanka C X, Hernández-Pérez M, Rabanal R M. Evaluation of the analgesic and topical anti-inflammatory effects of Hypericum reflexum L. fil. J Ethnopharmacol 2006; 107: 1-6. [DOI:10.1016/j.jep.2006.01.032]
44. Scheinfeld N. Phenytoin in cutaneous medicine: its uses, mechanisms and side effects. Dermatol Online J 2003; 9: 6. [DOI:10.5070/D32197W4T4]
45. Schempp C M, Pelz K, Wittmer A, Schöpf E, Simon J C. Antibacterial activity of hyperforin from St John’s wort, against multiresistant Staphylococcus aureus and gram-positive bacteria. Lancet 1999; 353: 2129. [DOI:10.1016/S0140-6736(99)00214-7]
46. Singh J, Kakkar P. Antihyperglycemic and antioxidant effect of Berberis aristata root extract and its role in regulating carbohydrate metabolism in diabetic rats. J Ethnopharmacol 2009; 123: 22-26. [DOI:10.1016/j.jep.2009.02.038]
47. Sorg H, Sorg C G G. Skin wound healing: of players, patterns, and processes. European Surgical Research 2022; 64: 141-157. [DOI:10.1159/000528271]
48. Sulakhiya K, Soni P, Tembhre M K, Kungumaraj H J, Paliwal R, Kumar S. 2 - Physiology and pharmacology of wounds. In: Solanki PR, Kumar A, Pratap Singh R, Singh J and Rb Singh K, editors. Nanotechnological aspects for next-generation wound management: Academic press, 2024: 21-54. [DOI:10.1016/B978-0-323-99165-0.00011-3]
49. Süntar I P, Akkol E K, Yilmazer D, Baykal T, Kirmizibekmez H, Alper M, et al. Investigations on the in vivo wound healing potential of Hypericum perforatum L. J Ethnopharmacol 2010; 127: 468-77. [DOI:10.1016/j.jep.2009.10.011]
50. Swoboda L, Held J. Impaired wound healing in diabetes. J Wound Care 2022; 31: 882-885. [DOI:10.12968/jowc.2022.31.10.882]
51. Torra i Bou J-E, Rueda López J, Segovia Gómez T, Bermejo Martínez M. [Topical administration of an hyperoxygenated fatty acid compound. Preventive and curative effects on pressure ulcer]. Revista de enfermeria (Barcelona, Spain) 2003; 26: 54-61.
52. World Health O. Definition, diagnosis and classification of diabetes mellitus and its complications : report of a WHO consultation. Part 1, Diagnosis and classification of diabetes mellitus. Journal 1999.
53. Yu M, Lee J Y. Polydeoxyribonucleotide improves wound healing of fractional laser resurfacing in rat model. Journal of Cosmetic and Laser Therapy 2017; 19: 43-48. [DOI:10.1080/14764172.2016.1247966]
| Rights and permissions | |
 |
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License. |
