Accepted Manuscripts
Back to the articles list |
Back to browse issues page
Mohammad Rahman Rahimi 
, Sanaz Mahmazi , Mehdi Abbaspoor , Mehdi Rahnema , Dara Latif Saifalddin , Nasser Ghanbari , Sarkawt Kolahdouzi , Hadi Golpasandi
, Sanaz Mahmazi , Mehdi Abbaspoor , Mehdi Rahnema , Dara Latif Saifalddin , Nasser Ghanbari , Sarkawt Kolahdouzi , Hadi Golpasandi
Abstract: (224 Views)
Introduction: Caffeine, as a popular drink, along with exercise training may restore altered genes in high-fat diet-induced obesity (HFD). This study examined the effects of exercise training, caffeine consumption, and their interaction on inflammation and genes involved in the metabolism in rats fed an HFD.
Methods: Eighty male Wistar rats were separated into two groups: HFD and normal diet (ND), and then each group was subsequently divided into four groups: sedentary, caffeine-only, exercise, and caffeine-plus-exercise. For eight weeks, the animals in the training groups engaged in aerobic exercise on a motorized treadmill for 60 minutes, five times per week. Animals in the caffeine group ingested a solution containing caffeine daily (6 mg/kg/bw). The expression of PGC 1a and FNDC1 genes in the calf muscle, UCP-1 in subcutaneous adipose tissue, NF-KB and TLR4 in visceral adipose tissue, and Fet-A in the liver were investigated.
Results: The findings demonstrated that HFD significantly elevated the NF-Kβ gene and downregulated the skeletal muscle PGC-1α and FNDC5 genes, as well as serum fetuin-A. UCP-1 (366% vs. 56%), FNDC5 (26% vs. 54%), and PGC-1α (40% vs. 1700%) genes were all considerably elevated by exercise training and caffeine supplementation, respectively. Additionally, exercise training reduced TLR4 and NF-KB expression in visceral adipose tissue and liver fetuin-A gene expression. Furthermore, following HFD feeding, when compared to the sedentary group, exercise training with and without caffeine consumption decreased the NF-Kβ gene and liver fetuin-A and increased PGC1-α, FNDC5, UCP1, and serum fetuin-A.
Conclusion: These findings support the idea that exercise and caffeine may reduce inflammation by downregulating genes involved in inflammation and adipose tissue browning.
Methods: Eighty male Wistar rats were separated into two groups: HFD and normal diet (ND), and then each group was subsequently divided into four groups: sedentary, caffeine-only, exercise, and caffeine-plus-exercise. For eight weeks, the animals in the training groups engaged in aerobic exercise on a motorized treadmill for 60 minutes, five times per week. Animals in the caffeine group ingested a solution containing caffeine daily (6 mg/kg/bw). The expression of PGC 1a and FNDC1 genes in the calf muscle, UCP-1 in subcutaneous adipose tissue, NF-KB and TLR4 in visceral adipose tissue, and Fet-A in the liver were investigated.
Results: The findings demonstrated that HFD significantly elevated the NF-Kβ gene and downregulated the skeletal muscle PGC-1α and FNDC5 genes, as well as serum fetuin-A. UCP-1 (366% vs. 56%), FNDC5 (26% vs. 54%), and PGC-1α (40% vs. 1700%) genes were all considerably elevated by exercise training and caffeine supplementation, respectively. Additionally, exercise training reduced TLR4 and NF-KB expression in visceral adipose tissue and liver fetuin-A gene expression. Furthermore, following HFD feeding, when compared to the sedentary group, exercise training with and without caffeine consumption decreased the NF-Kβ gene and liver fetuin-A and increased PGC1-α, FNDC5, UCP1, and serum fetuin-A.
Conclusion: These findings support the idea that exercise and caffeine may reduce inflammation by downregulating genes involved in inflammation and adipose tissue browning.
Keywords: Exercise training, Caffeine, Supplementation, Brown Adipose Tissue Uncoupling Protein, Inflammation
Type of Manuscript: Experimental research article |
Subject:
Others
| Rights and permissions | |
 |
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License. |
