Volume 11, Issue 3 (Fall 2007)
Physiol Pharmacol 2007, 11(3): 208-217 |
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Bigdeli M R, Hajizadeh S, Frouzandeh M, khoshbaten A. Study on the effect of neuroprotective prolonged and intermittent normobaric hyperoxia on serum level of TNF-α and glutamate transporters expression in rat brain. Physiol Pharmacol 2007; 11 (3) :208-217
URL: http://ppj.phypha.ir/article-1-269-en.html
URL: http://ppj.phypha.ir/article-1-269-en.html
Abstract: (15286 Views)
Introduction: Prolonged and intermittent oxygen pre-exposure is associated with protection against ischemic
reperfusion (IR) injury. In the current study, attempts were made to investigate the relationship between exposure to
prolonged and intermittent normobaric hyperoxia (NBHO) and expression of excitatory amino acids transporters
(EAATs) and TNF-α level.
Method: Rats were divided into four main experimental groups, each of 21 animals. The first two were exposed to
95% inspired NBHO 4 h/day for 6 consecutive days (intermittent NBHO) or for 24 continuous hours (prolonged NBHO).
The second two groups considered as controls and were exposed to 21% oxygen in the same chamber (normobaric
normoxia, NBNO). Each main group was subdivided to MCAO (middle cerebral artery occlusion), sham-operated
(without MCAO) and intact (without any surgery) subgroups. After 24h, MCAO subgroups were subjected to 60 min of
right MCAO. After 24 h reperfusion, neurologic deficit scores (NDS) were assessed in MCAO-operated subgroups.
Immediately and 48 h after pretreatment, blood sampling were done for assessing level of serum TNF-α. The effect of
intermittent and prolonged NBHO on EAATs expression level was also measured using western blotting.
Result: Preconditioning with prolonged and intermittent NBHO decreased NDS and up-regulated EAAT1, EAAT2, and
EAAT3, significantly. Also, oxygen exposure of prolonged and intermittent NBHO increased the level of serum TNF-α.
Conclusion: Although further studies are needed to clarify the protective mechanisms OF hyperoxia, the
intermittent and prolonged NBHO seems to partly exert their effects via increasing the serum level of TNF-α and upregulation
of glutamate transporters. However, the intermittent NBHO seems to have appropriate effects with low
toxicity.
Keywords: Normobaric Hyperoxia, Brain ischemic tolerance, Stroke, Neuroprotection, Glutamate transporter, TNF-α, Rat
Type of Manuscript: Experimental research article |
Subject:
Cardiovascular Physiology/Pharmacology
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