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Introduction: Di-2-ethylhexyl phthalate (DEHP) caused the osteoblasts mortality in vitro. Since the rat bone marrow mesenchymal stem cells (MSCs) is the osteoblasts cellular back up, we studied the effect of DEHP on viability, proliferation and some biochemical factors of MSCs. Methods:  MSCs were treated with 0.5 to 2500 μM of DEHP for 12, 24 and 48 hours to study the viability. Then further investigations, including proliferation, cell morphology, sodium and potassium level, concentration of calcium, total protein, activity of metabolic enzymes (ALT,AST,ALP,LDH), malondialdehyde (MDA) level, total antioxidant capacity(TAC), activity of superoxide dismutase(SOD) and catalase(CAT) were measured using selected concentration of 100, 500 and 1500 μM for 48 hours. Results: 100 μM of DEHP did not change the viability and biochemical factor after 48 hours but CFA and PDN was significantly affected. 500 μM only reduced the viability at 24 and 48 hours while 1500 μM caused the reduction at all the periods. These two concentrations, caused proliferation reduction and increased calcium and sodium level, LDH activity and MDA level. While decreased potassium, total protein, activity of metabolic enzymes and activity of CAT and SOD. Conclusion: DEHP has reduced viability and proliferation of MSCs through metabolic change, alteration in cellular ultrastructure, ionic imbalance and induction of oxidative stress.
     
Types of Manuscript: Original Research | Subject: Cellular and Molecular BioMedicine

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