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Negin Givechian 
, Ghorbangol Ashabi , Mehri Kadkhodaee , Behjat Seifi , Arash Abdi , Farzaneh Kianian , Tina Kianfar
, Ghorbangol Ashabi , Mehri Kadkhodaee , Behjat Seifi , Arash Abdi , Farzaneh Kianian , Tina Kianfar
Abstract: (463 Views)
Introduction: Acute kidney injury (AKI) is a severe complication of rhabdomyolysis (RM). RM occurs by skeletal muscle injury in which cell contents are released into blood circulation and finally obstructs renal tubules. Renal dysfunction happens consequently, due to an increase in oxidative stress, inflammation, and apoptosis. Glycerol (10 mL/kg) injection is one of the most common methods to induce experimental AKI.
Methods: 28 male Wistar rats were used in our investigation. We first studied to find the most effective dosage of glycerol for AKI induction in three groups (5, 6.25, and 10 mL/kg), and accordingly 6.25 mL/kg was selected. Secondly, we investigated isoflurane (1.5%, 20 minutes) pretreatment effects on glycerol-induced AKI by estimating blood urea nitrogen (BUN), creatinine (Cr), Bcl-2-associated X (Bax) and B-cell lymphoma 2 (Bcl-2) proteins, malondialdehyde (MDA), superoxide dismutase (SOD), and histological changes in renal tissues.
Results: Results showed that glycerol (6.25 mL/kg) injection increased blood BUN, Cr and also, elevated renal Bax/Bcl-2 ratio and MDA level and decreased renal SOD level in comparison to sham group (P<0.001 for all). Isoflurane pretreatment suppressed blood BUN, Cr and decreased renal Bax/Bcl-2 ratio and MDA level and enhance SOD level compared to glycerol group (P<0.001 for all).
Conclusion: Isoflurane pretreatment might be protective against rhabdomyolysis-induced AKI because of its anti-oxidant and anti-apoptotic activities.
Methods: 28 male Wistar rats were used in our investigation. We first studied to find the most effective dosage of glycerol for AKI induction in three groups (5, 6.25, and 10 mL/kg), and accordingly 6.25 mL/kg was selected. Secondly, we investigated isoflurane (1.5%, 20 minutes) pretreatment effects on glycerol-induced AKI by estimating blood urea nitrogen (BUN), creatinine (Cr), Bcl-2-associated X (Bax) and B-cell lymphoma 2 (Bcl-2) proteins, malondialdehyde (MDA), superoxide dismutase (SOD), and histological changes in renal tissues.
Results: Results showed that glycerol (6.25 mL/kg) injection increased blood BUN, Cr and also, elevated renal Bax/Bcl-2 ratio and MDA level and decreased renal SOD level in comparison to sham group (P<0.001 for all). Isoflurane pretreatment suppressed blood BUN, Cr and decreased renal Bax/Bcl-2 ratio and MDA level and enhance SOD level compared to glycerol group (P<0.001 for all).
Conclusion: Isoflurane pretreatment might be protective against rhabdomyolysis-induced AKI because of its anti-oxidant and anti-apoptotic activities.
Type of Manuscript: Short communication |
Subject:
Renal Physiology/Pharmacology
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