Volume 29, Issue 4 (December 2025)
Physiol Pharmacol 2025, 29(4): 408-423 |
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Mousavinezhad S A, Harzandi N, Marjani A, Jafari P. Evaluation of the effect of biodegradable dressing prepared from chitosan and external microvesicles of Bifidobacterium bifidum on burn wound healing in male Wistar rats. Physiol Pharmacol 2025; 29 (4) :408-423
URL: http://ppj.phypha.ir/article-1-2417-en.html
URL: http://ppj.phypha.ir/article-1-2417-en.html
Abstract: (1837 Views)
Introduction: The burn wound healing process is multi-variable, and various factors are involved. Chitosan-based dressings are acceptable materials for wound healing. PProbiotics and postbiotics can in-crease the effectiveness of these dressings. This study aimed to investigate the effect of a biodegradable dressing prepared from chitosan and external microvesicles of Bifidobacterium bifidum on burn wound healing in male Wistar rats. Methods: Bifidobacterium bifidum was cultured, the activated crude supernatant (ACS) was separated, and the microvesicles were isolated with the help of Ultra-centrifugation at 150,000×g at 4°C for 30 minutes. Chitosan films containing and without microvesicles were prepared using a heater stirrer at 29°C. The effectiveness of the membranes was evaluated. For this purpose, 60 male rats with second-degree burns were randomly divided into 5 groups (n=12) and were treated for 21 days. The process of wound healing was examined macroscopically and microscopically (wound histology and evaluation of gene expression of cytokines interleukin-8, interleukin-10, and VEGF) on the days 3, 7, 14 and 21. The obtained data were analyzed by IBM SPSS.21 software and using Kolmogorov-Smirnov, Kruskal-Wallis, and Mann-Whitney tests with p ≤ 0.05. Results: The average diameter of microvesicles was 174 ± 52nm. The thickness of the layers was 2mm. The wounds of the group post-biotic and positive control were closed on the 14th day. Collagen production and epithelialization, as well as inflammation control in the treatment groups, were higher than negative control. Conclusion: The use of chitosan membranes, particularly those enhanced with ACS and microvesicles, represents a significant advancement in wound care by inflammation control, offering a promising strategy for improving the healing of burn wounds.
Type of Manuscript: Experimental research article |
Subject:
Clinical Physiology/Pharmacology
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