Volume 30, Issue 2 (June 2026)                   Physiol Pharmacol 2026, 30(2): 210-226 | Back to browse issues page

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moazami M, Hayati-Roodbari N, Parivar K, Kamalinejad M, kaka G. Repair of cutaneous sciatic nerve by using bone marrow stromal cells on a chitosan-gelatin membrane with olive fruit extract in rats. Physiol Pharmacol 2026; 30 (2) :210-226
URL: http://ppj.phypha.ir/article-1-2594-en.html
Repair of cutaneous sciatic nerve by using bone marrow stromal cells on a chitosan-gelatin membrane with olive fruit extract in rats
Mostafa Moazami , Nasim Hayati-Roodbari , Kazem Parivar , Mohammad Kamalinejad , Gholamreza Kaka
Abstract:   (980 Views)

Introduction: Despite various treatment methods, the damaged peripheral nervous system has become a severe problem in today’s societies. This study aimed to evaluate the effects of cultured bone marrow stromal cells (BMSCs) on the chitosan-gelatin membrane and olive fruit extract in Wistar rat cutaneous sciatic nerve repair.
Methods: The chitosan-gelatin membrane was prepared after the extraction of olive oil, the extraction of BMSCs, and cultivation in special pellets. Wistar rats were divided to 9 groups (n=5): healthy control, sciatic nerve injury, nerve injury treated with bone marrow stromal stem cells, nerve injury treated with olive fruit extract, nerve injury treated with olive fruit extract+bone marrow stromal stem cells, nerve injury treated with olive fruit extract+chitosan-gelatin, nerve injury treated with chitosan-gelatin+ bone marrow stromal stem cells, and nerve injury treated with olive fruit extract+chitosan-gelatin+ bone marrow stromal stem cells. The treatment period was 8 weeks, then the evaluation of sciatic nerve damage repair included electrophysiological, sciatic functional index, tissue sampling, immunocytochemistry, and histopathological examination.
Results: In the group treated with olive fruit extract +chitosan-gelatin+ bone marrow stromal stem cells, sciatic nerve repair was observed with an increase in nerve conduction velocity (m/s), amplitude muscle action potential (mv), number of (vessels and fiber), most significant area of vessels, myeline, axon, and fiber (1000 micro m2) and a decrease in statistic functional index (0 to -50) (P<0.0001).
Conclusion: The combination of olive extract, chitosan-gelatin, and bone marrow stromal stem cells (BMSCs) demonstrated a significant reparative effect on sciatic nerve injury and could serve as a promising approach for peripheral nerve repair.

Keywords: Peripheral nervous system, Sciatic nerve, Chitosan-gelatin scaffold, Olive fruit extract, Bone marrow stromal stem cells
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Type of Manuscript: Experimental research article | Subject: Cell, Stem Cell and Cancer
References
1. Agatonovic-Kustrin S, Gegechkori V. HPTLC and FTIR Fingerprinting of Olive Leaves Extracts and ATR-FTIR Characterisation of Major Flavonoids and Polyphenolics. Molecules 2021; 26: 6892. [DOI:10.3390/molecules26226892]
2. Becker K, Kienecker E, Andrae I. Effect of locally applied corticoids on the morphology of peripheral nerves following neurotmesis and microsurgical suture. Neurochirurgia 1987; 30: 161-167. [DOI:10.1055/s-2008-1054088]
3. Boka V-I, Argyropoulou A, Gikas E, Angelis A, Aligiannis N, Skaltsounis A-L. Employment of high-performance thin-layer chromatography for the quantification of oleuropein in olive leaves and the selection of a suitable solvent system for its isolation with centrifugal partition chromatography. Planta Medica 2015; 81: 1628-1635. [DOI:10.1055/s-0035-1558140]
4. Cho Y, Shi R, Borgens R B. Chitosan produces potent neuroprotection and physiological recovery following traumatic spinal cord injury. Journal of Experimental Biology 2010; 213: 1513-1520. [DOI:10.1242/jeb.035162]
5. Choi B-H, Zhu S-J, Kim B-Y, Huh J-Y, Lee S-H, Jung J-H. Transplantation of cultured bone marrow stromal cells to improve peripheral nerve regeneration. International Journal of Oral and Maxillofacial Surgery 2005; 34: 537-542. [DOI:10.1016/j.ijom.2004.10.017]
6. Cui L, Jiang J, Wei L, Zhou X, Fraser J L, Snider B J, et al. Transplantation of embryonic stem cells improves nerve repair and functional recovery after severe sciatic nerve axotomy in rats. Stem Cells 2008; 26: 1356-1365. [DOI:10.1634/stemcells.2007-0333]
7. Diegelmann R F, Dunn J D, Lindblad W J, Cohen I K. Analysis of the effects of chitosan on inflammation, angiogenesis, fibroplasia, and collagen deposition in polyvinyl alcohol sponge implants in rat wounds. Wound Repair and Regeneration 1996; 4: 48-52. [DOI:10.1046/j.1524-475X.1996.40109.x]
8. Eckersley L, Ansselin A D, Tomlinson D R. Effects of experimental diabetes on axonal and Schwann cell changes in sciatic nerve isografts. Molecular Brain Research 2001; 92: 128-137. [DOI:10.1016/S0169-328X(01)00163-2]
9. Falvo E, Diviccaro S, Melcangi R C, Giatti S. Physiopathological role of neuroactive steroids in the peripheral nervous system. International Journal of Molecular Sciences 2020; 21: 9000. [DOI:10.3390/ijms21239000]
10. Fan B-S, Lou J-Y. Enhancement of angiogenic effect of co-transfection human NGF and VEGF genes in rat bone marrow mesenchymal stem cells. Gene 2011; 485: 167-171. [DOI:10.1016/j.gene.2011.06.027]
11. Gaspar-Pintiliescu A, Stanciuc A-M, Craciunescu O. Natural composite dressings based on collagen, gelatin and plant bioactive compounds for wound healing: A review. International Journal of Biological Macromolecules 2019; 138: 854-865. [DOI:10.1016/j.ijbiomac.2019.07.155]
12. Jayaraman K, Kotaki M, Zhang Y, Mo X, Ramakrishna S. Recent advances in polymer nanofibers. Journal of Nanoscience and Nanotechnology 2004; 4: 52-65.
13. Kato K, Liu H, Kikuchi Si, Myers R R, Shubayev V I. Immediate anti-tumor necrosis factor-α (etanercept) therapy enhances axonal regeneration after sciatic nerve crush. Journal of Neuroscience Research 2010; 88: 360-368. [DOI:10.1002/jnr.22202]
14. Lee KS, Baek J R, Lee G H, Choi G W. Comparative study of scar formation at the site of sciatic nerve repair in rats. Journal of the Korean Orthopaedic Association 2007; 42: 162-170. [DOI:10.4055/jkoa.2007.42.2.162]
15. Muzzarelli R, Mattioli-Belmonte M, Pugnaloni A, Biagini G. Biochemistry, histology and clinical uses of chitins and chitosans in wound healing. Experientia Supplementum 1999; 87: 251-264. [DOI:10.1007/978-3-0348-8757-1_18]
16. Navarro X, Vivó M, Valero-Cabré A. Neural plasticity after peripheral nerve injury and regeneration. Progress in Neurobiology 2007; 82: 163-201. [DOI:10.1016/j.pneurobio.2007.06.005]
17. Nikhal S, Dambe P, Ghongade D, Goupale D. Hydroalcoholic extraction of Mangifera indica (leaves) by Soxhletion. International Journal of Pharmaceutical Sciences 2010; 2: 30-32.
18. Ren Z, Wang Y, Peng J, Zhao Q, Lu S. Role of stem cells in the regeneration and repair of peripheral nerves. Reviews in the Neurosciences 2012; 23: 135-143. [DOI:10.1515/revneuro-2011-0069]
19. Shishido H, Kikuchi S, Heckman H, Myers R R. Dexamethasone decreases blood flow in normal nerves and dorsal root ganglia. Spine 2002; 27: 581-586. [DOI:10.1097/00007632-200203150-00005]
20. Smith D, Tweed C, Fernyhough P, Glazner G W. Nuclear factor-κB activation in axons and Schwann cells in experimental sciatic nerve injury and its role in modulating axon regeneration: studies with etanercept. Journal of Neuropathology & Experimental Neurology 2009; 68: 691-700. [DOI:10.1097/NEN.0b013e3181a7c14e]
21. Swett J E, Hong C Z, Miller P G. All peroneal motoneurons of the rat survive crush injury but some fail to reinnervate their original targets. Journal of Comparative Neurology 1991; 304: 234-252. [DOI:10.1002/cne.903040207]
22. Teixeira A I, Abrams G A, Bertics P J, Murphy C J, Nealey P F. Epithelial contact guidance on well-defined micro-and nanostructured substrates. Journal of Cell Science 2003; 116: 1881-1892. [DOI:10.1242/jcs.00383]
23. Temporin K, Tanaka H, Kuroda Y, Okada K, Yachi K, Moritomo H, et al. Interleukin-1 beta promotes sensory nerve regeneration after sciatic nerve injury. Neuroscience Letters 2008; 440: 130-133. [DOI:10.1016/j.neulet.2008.05.081]
24. Varier P, Raju G, Madhusudanan P, Jerard C, Shankarappa S A. A brief review of in vitro models for injury and regeneration in the peripheral nervous system. International Journal of Molecular Sciences 2022; 23: 816. [DOI:10.3390/ijms23020816]
25. Yardim A, Kandemir F M, Ozdemir S, Kucukler S, Comakli S, Gur C, et al. Quercetin provides protection against the peripheral nerve damage caused by vincristine in rats by suppressing caspase 3, NF-κB, ATF-6 pathways and activating Nrf2, Akt pathways. Neurotoxicology 2020; 81: 137-146. [DOI:10.1016/j.neuro.2020.10.001]
26. Zarbakhsh S, Bakhtiyari M, Faghihi A, Joghataei M T, Mehdizadeh M, Khoei S, et al. The effects of schwann and bone marrow stromal stem cells on sciatic nerve injury in rat: a comparison of functional recovery. Cell Journal (Yakhteh) 2012; 14: 39.
27. Zhelyaznik N, Schrage K, McCaffery P, Mey J. Activation of retinoic acid signalling after sciatic nerve injury: up-regulation of cellular retinoid binding proteins. European Journal of Neuroscience 2003; 18: 1033-1040. [DOI:10.1046/j.1460-9568.2003.02834.x]
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