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Abstract:   (17 Views)
Introduction: Astrocyte, S100B, and nitric oxide may have a role in the pathogenesis and treatment of epilepsy. However, the effects of nitric oxide and S100B on the gliotoxic effects of chemical convulsants such as pentylenetetrazole is unknown. Therefore, we aimed to evaluate the effects of S100B and nitric oxide on gliotoxicity of pentylenetetrazole in a 1321NI1 astrocytic culture.
Methods: 1321N1 astrocytes were exposed to pentylenetetrazole (40mM) and arundic acid (50 µM), or to both of them for 24h. In addition, we poured L-arginine (100 and 500μM), N-Nitro-L--arginine methyl ester (100 and 500μM), 7-nitroindazole (30 and 100μM), and aminoguanidine (50 and 100μM) to the culture media contained pentylenetetrazole, arundic acid or both of them and incubated for 24h. Cell viability was measured by the methylthiazolyldiphenyl-tetrazolium bromide reagent and the S100B protein level was measured by using an enzyme-linked immunosorbent assay.
Results: There was a negative correlation between cell viability in astrocytes and the intracellular S100B levels. Pentylenetetrazole decreased cell viability, but it increased the intracellular S100B levels.  Arundic acid, N-Nitroarginine methyl ester, 7-nitroindazole, and aminoguanidine reversed the Pentylenetetrazole effects on cell viability and intracellular S100B levels. Adding the L-arginine to Pentylenetetrazole plus arundic acid reduced the modulatory effects of arundic acid on pentylenetetrazole.
Conclusion: Nitric oxide and S100B have a role in gliotoxicity of pentylenetetrazole in cell culture. Arundic acid suppresses pentylenetetrazole-induced S100B elevation and gliotoxicity possibly by modulation of the nitric oxide pathway.
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Types of Manuscript: Original Research | Subject: Cellular and Molecular BioMedicine