Volume 23, Issue 3 (September 2019)                   Physiol Pharmacol 2019, 23(3): 150-153 | Back to browse issues page

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Farrokhi M R, Emamghoreishi M, Amiri A, Keshavarz M. Neuroprotective effects of caffeine against beta-amyliod neurotoxicity: The involvement of glycogen synthase kinase-3β protein. Physiol Pharmacol. 2019; 23 (3) :150-153
URL: http://phypha.ir/ppj/article-1-1447-en.html
Abstract:   (389 Views)
Introduction: The reduction of glycogen synthase kinase-3β protein level may correlate to the neuroprotective effects of antioxidant agents like caffeine. Therefore, we aimed to evaluate the impact of GSK-3β protein on neuroprotective effects of caffeine in the SHSY5Y cells exposed to beta-amyloid. Methods: We incubated SHSY5Ycells with beta-amyloid 25–35 and caffeine (0.6 and 1mM) for 24h. Cell viability was determined using MTT test. We used the western blotting technique to measure the glycogen synthase kinase-3β and phosphorylated glycogen synthase kinase-3β protein levels. Results: Caffeine (0.6 and 1mM) diminished beta-amyloid neurotoxicity and attenuated the beta-amyloid effects on the glycogen synthase kinase-3β protein level in a neuronal culture. Conclusion: Caffeine neuroprotective effects against beta-amyloid may correlate to glycogen synthase kinase-3β protein.
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