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Abstract:   (117 Views)
Introduction: In this study, we investigated the effect of basil rosmarinic acid-rich extract on mice lipid metabolism, low-density lipoprotein oxidation and antiradical property.
Methods: The rosmarinic acid rich-extract was used to treat male mice. Mice were divided into four groups of seven mice and treatment was performed daily and orally for 9 weeks. Hypolipidemic effect was evidenced by the measurement of plasma and liver lipid profile. Thiobarbituric acid reactive substances assay was used to measure the antioxidant capacity of the phenolic extract using mice plasma rich in LDL. The antioxidant activity of the extract was assessed by 2,2-diphenyl-1-picrylhydrazyl free radical scavenging capacity and the bleaching analysis β-carotene.
Results: The extract exerts a significant decrease in plasma total cholesterol (P= 0.011), triglycerides (P<0.05) and LDL-cholesterol (P<0.001). Similar results were observed in liver total cholesterol (P<0.01) and triglycerides (P<0.01). The phenolic extract prevents lipoprotein oxidation by 93% (P<0.001) at a dose of 25 µg/ml. We also note that the extract scavenge DPPH radical in a dose-dependent manner with an IC50= 12.45 ± 0.18 µg/ml. Besides, the extract inhibits oxidation process of β-carotene, the IC50 was 12.16 ± 0.19 µg/ml. HPLC analysis shows that the extract contains caftaric acid (2.9%), caffeic acid (4.3%), chicoric acid (5.5%) and rosmarinic acid (87.3%) which is the major compound.
Conclusion: The results obtained suggest that the extract may be an important source of natural compounds that can be exploited as a substrate to develop a new treatment of hyperlipidemia and atherosclerosis.
     
Types of Manuscript: Original Research | Subject: Pharmacology