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Abstract:   (152 Views)
Introduction: Spinal cord injury is a condition which can lead to permanent loss of neurons, glial, and precursor cells. According to the positive influences of electrical stimulation in the neurogenesis, we hypothesized that sub-threshold electrical stimulation may stimulate the differential regulation of wingless-type3(Wnt-3), and eukaryotic Initiation Factor-2α(eIF2α) mediators in spinal cord injured rats. Methods: Forty male Wistar rats (weighing 250-280 g) were randomly divided into four groups; sham, spinal cord injury, spinal cord injury+astrocyte, and spinal cord injury+astrocyte which followed by electrical stimulation. We evaluated the glial fibrillary acidic protein (GFAP), doublecortin, Wnt-3, and eIF2α proteins by immunofluorescence and immunoblotting techniques. Results: The results show that expression of Wnt-3 and eIF2α proteins significantly enhanced after 14 days in the electrical stimulation+spinal cord injury+astrocyte group in comparison with spinal cord injury and spinal cord injury+Astrocyte groups. Also, the expression of GFAP cells was significantly increased after 14 days by electrical stimulation compared with other groups. Conclusion: Electrical stimulation had no effect on expression of doublecortin after14 days. This survey confirmed that sub-threshold-electrical stimulation upregulates neurogenesis mediators, but there are no evidences based on doublecortin expression as a neurogenesis biomarker.
Types of Manuscript: Original Research | Subject: Neurodegeneretive diseases