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Abstract:   (84 Views)
Abstract
Introduction: Cisplatin induced nephrotoxicity may limit the clinical use of this drug. The aim of this study was to investigate the mechanism of the possible renoprotective effect of curcumin in cisplatin nephrotoxicity.
Material and Methods: Thirty male Wistar rats were randomly divided into five groups: 1- control (normal saline, 0.5 ml ip., daily for 10 days); 2- cisplatin (10 mg/kg ip, single dose at the first day); 3- cisplatin + curcumin (10 mg/kg ip, dissolved in 5% DMSO, daily for 10 days); 4- cisplatin + vehicle (DMSO 5%, 0.3 ml ip.); and 5- curcumin (10 mg/kg ip.). At the end of the study, urine and blood samples were obtained for biochemical analysis. The right kidneys were kept in 10% formalin for (H&E and TUNEL) staining and the left kidneys were kept at -80 ° C for type 2 organic cation transporter (OCT2) and type 3 glutathione peroxidase (GPx3) gene expression and MDA measurements.
Results: Cisplatin significantly increased serum Cr, BUN, K and kidney lipid peroxidation (p<0.01). However, the effect of cisplatin on Gpx3 and OCT2 gene expression was not statistically significant. Curcumin treatment decreased serum Cr, BUN, and kidney lipid peroxidation, but increased GPx3 and OCT2 gene expression (p<0.01). Moreover, curcumin significantly reversed the cisplatin-induced kidney tissue injury and decreased apoptosis.
Conclusion: It is concluded that the ameliorative effect of curcumin in cisplatin nephrotoxicity was assumed to be due to antioxidant effect of this agen. Although the role of curcumin in mediating uptake of cisplatin is still unclear.
 
     
Types of Manuscript: Original Research | Subject: Kidney