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Abstract:   (188 Views)
Introduction: Extracellular signal-regulated kinase 1 and 2 (ERK1/2) are important members of mitogen-activated protein kinases (MAPK), which are actively involved in the shaping of cellular responses to different stimuli, however, these responses, are somehow contradicting. It is believed that time is a crucial factor in the determination of these effects; therefore, the present work was designed to obtain a more vivid view about the effect of time on ERK activity and its relation to cell viability. Methods: In the first step, we challenged cultured PC12 cells with different doses of Lipopolysaccharides (LPS) for different time intervals (3,6,24 and 48 hours) and the cell viability was checked by MTT test. Thereafter, we cultured the cells in 6-well plates and treated them with the effective dose (10µg/ml) for the abovementioned intervals, and the level of ERK phosphorylation, as the active form, was assessed in the Western blotting analysis. Results: The results showed that treating cells with 10µg/ml LPS reduces cell viability after 48 hours. While being ineffective in shorter periods of time, ERK activity has a fluctuating trend, so that it reaches the highest level at 6 hours, thereafter it declines to the lowest level at 24 hours and partially increases again at 48 hours. Conclusion: These results imply that time is a determinant factor in the activity of ERK and single-point assessments may result in misinterpretation.
Types of Manuscript: Original Research | Subject: Cellular and Molecular BioMedicine