Volume 26, Issue 1 (March 2022)                   Physiol Pharmacol 2022, 26(1): 1-6 | Back to browse issues page

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Abolmaali M, Motevalian M, Mehrzadi S, Shojaii A. Anticonvulsant effects of squill oxymel (a traditional formulation) in mice. Physiol Pharmacol. 2022; 26 (1) :1-6
URL: http://ppj.phypha.ir/article-1-1694-en.html
Abstract:   (1258 Views)
Introduction: This study evaluated the anticonvulsant activity of the add-on Drimia maritima (squill) oxymel, used traditionally in the treatment of convulsion, in animal model. Methods: Albino mice pretreated with squill oxymel in different doses of 50, 100, 200 and 400mg/kg by oral gavage, 15min prior to injection of pentylenetetrazole (PTZ). Animals pretreated with flumazenil to determine the mechanism of anticonvulsant action. The total flavonoid content of squill oxymel was also determined. Results: Squill oxymel prolonged the onset of seizures and decreased the duration of seizures compared to control group. Diazepam used as a reference drug for its anticonvulsive effects, showed complete inhibition of seizure. This study revealed that squill oxymel has significant anticonvulsant effect in PTZ-induced seizures in mice and these effects may be related to its effect on benzodiazepines’ receptors on GABA complex. Conclusion: These results confirmed the traditional use of squill oxymel in Iranian traditional medicine for treatment of epilepsy. The clarification of mechanisms involved needs further studies.
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1. Aghili Khorasani M., 1771. Makhzan-al-adviah (In Persian), edited by: Rahimi R, Farjadmand F, Shams Ardakani MR. Tehran University of medical sciences. 2009; 130-132.
2. Avicenna. The canon of Medicine. Tehran: Soroush Press, 2008.
3. Belhaddad OE, Charef N, Amamra S, Zerargui F, Baghiani A, Khennouf S, et al. Chromatographic fractionation, antioxidant and antibacterial activities of Urginea maritima methanolic extract. Pak J Pharm Sci 2018. 30: 127-34
4. Bozorgi M, Amin G, Shekarchi M, Rahimi R. Traditional medical uses of Drimia species in terms of phytochemistry, pharmacology and toxicology. J Tradit Chin Med 2017; 37: 124-39. [DOI:10.1016/S0254-6272(17)30036-5]
5. Chamberlain FL, Levy RL. Clinical study of a preparation of squill (urginin) in the treatment of myocardial insufficiency. Am Heart J 1937; 14: 268-83. [DOI:10.1016/S0002-8703(37)90564-7]
6. Corda M, Giorgi O, Longoni B, Orlandi M, Biggio G. Decrease in the function of the γ‐aminobutyric acid‐ coupled chloride channel produced by the repeated administration of pentylenetetrazol to rats. J Neurochem 1990; 55: 1216-21. [DOI:10.1111/j.1471-4159.1990.tb03127.x]
7. Dizaye KF, Hamad BA. Cardiovascular studies of white squill (Urginea maritima) extract. Zanco J Med Sci 2010; 14: 20-7.
8. Greenberg AE. Standard methods for the examination of water and wastewater. Washington DC, American Public Health Association. 1981.
9. Haile M, Kang WH. Antioxidant activity, total polyphenol, flavonoid and tannin contents of fermented green coffee beans with selected yeasts. Fermentation 2019; 5: 29. [DOI:10.3390/fermentation5010029]
10. Johannessen Landmark C, Patsalos PN. Drug interactions involving the new second- and third-generation antiepileptic drugs. Expert Rev Neurother 2010; 10: 119-40. [DOI:10.1586/ern.09.136]
11. Kirmani BF, Robinson DM, Kikam A, Fonkem E, Cruz D. Selection of antiepileptic drugs in older people. Curr Treat Options Neurol 2014; 16: 295. [DOI:10.1007/s11940-014-0295-4]
12. Mammadov R, Makasçı-Afacan A, Uysal-Demir D, Görk Ç. Determination of antioxidant activities of different Urginea maritima (L.) Baker plant extracts. Iranian Journal of Chemistry and Chemical Engineering (IJCCE) 2010; 29: 47-53.
13. Mehrzadi S, Sadr S, Hosseinzadeh A, Gholamine B, Shahbazi A, FallahHuseini H, et al. Anticonvulsant activity of the ethanolic extract of Punica granatum L. seed. Neurol Res 2015; 37: 470-5. [DOI:10.1179/1743132814Y.0000000460]
14. Mehrzadi S, Shojaii A, Pur SA, Motevalian M. Anticonvulsant activity of hydroalcoholic extract of Citrullus colocynthis fruit: involvement of benzodiazepine and opioid receptors. Evid-Based Compl Alt 2016; 21: 31-5. [DOI:10.1177/2156587215615455]
15. Moshi MJ, Kagashe GA, Mbwambo ZH. Plants used to treat epilepsy by Tanzanian traditional healers. J Ethnopharmacol 2005; 97: 327-36. [DOI:10.1016/j.jep.2004.11.015]
16. Motevalian M, Mehrzadi S, Ahadi S, Shojaii A. Anticonvulsant activity of Dorema ammoniacum gum: evidence for the involvement of benzodiazepines and opioid receptors. Res Pharm Sci 2017; 12: 53-9. [DOI:10.4103/1735-5362.199047]
17. Nejatbakhsh F, Karegar-Borzi H, Amin G, Eslaminejad A, Hosseini M, Bozorgi M, et al. Squill Oxymel, a traditional formulation from Drimia Maritima (L.) Stearn, as an add-on treatment in patients with moderate to severe persistent asthma: A pilot, triple-blind, randomized clinical trial. J Ethnopharmacol 2017; 196: 186-92. [DOI:10.1016/j.jep.2016.12.032]
18. Ngugi AK, Bottomley C, Kleinschmidt I, Sander JW, Newton CR. Estimation of the burden of active and life-time epilepsy: a meta-analytic approach. Epilepsia 2010; 51: 883-90. [DOI:10.1111/j.1528-1167.2009.02481.x]
19. Obeidat M, Sharab A. Antimicrobial and anticancer activities of extracts from Urginea maritime fruits. Afr J Tradit Complement Altern Med 2018; 15: 74-84. [DOI:10.21010/ajtcam.v15i1.8]
20. Rashidian A, Farhang F, Vahedi H, Dehpour AR, Ejtemai Mehr S, Mehrzadi S, et al. Anticonvulsant effects of Lippia citriodora (Verbenaceae) leaves ethanolic extract in mice: role of GABAergic system. Int J Prev Med 2016; 7: 97. [DOI:10.4103/2008-7802.187251]
21. Rashidian A, Kazemi F, Mehrzadi S, Dehpour AR, Mehr SE, Rezayat SM. Anticonvulsant effects of aerial parts of verbena officinalis extract in mice: involvement of benzodiazepine and opioid receptors. J Evid Based Complementary Altern Med 2017; 22: 632-6. [DOI:10.1177/2156587217709930]
22. Riazi K, Honar H, Homayoun H, Rashidi N, Dehghani M, Sadeghipour H, et al. Sex and estrus cycle differences in the modulatory effects of morphine on seizure susceptibility in mice. Epilepsia 2004; 45: 1035-42. [DOI:10.1111/j.0013-9580.2004.69903.x]
23. Shahidi F, Ho CT. Antioxidant measurement and applications: an overview. J Am Chem Soc 2007: 2-7. [DOI:10.1021/bk-2007-0956.ch001]
24. Shams Ardakani MR, Farjadmand F, Rahimi R. Makhzan al Adviyeh and Pointing to the Scientific Names of Medicinal Plants for the First Time in a Persian Book. Trad Integr Med. 3(4):186-195.
25. Stannard J. Squill in ancient and medieval materia medica, with special reference to its employment for dropsy. Bull N Y Acad Med 1974; 50: 684-713.
26. Thurman DJ, Beghi E, Begley CE, Berg AT, Buchhalter JR, Ding D, et al. Standards for epidemiologic studies and surveillance of epilepsy. Epilepsia 2011; 52: 2-26. [DOI:10.1111/j.1528-1167.2011.03121.x]
27. Zargari A. Medicinal plants. Tehran: Tehran University of medical sciences publication, 1996.
28. Zhu HL, Wan JB, Wang YT, Li BC, Xiang C, He J, et al. Medicinal compounds with antiepileptic/anticonvulsant activities. Epilepsia 2014; 55: 3-16. [DOI:10.1111/epi.12463]

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