Volume 27, Issue 4 (December 2023)                   Physiol Pharmacol 2023, 27(4): 417-425 | Back to browse issues page

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Khajehnasiri N, hosseini M, Piraki P, Ghowsi M, Rahbarnia L, Habibi P et al . Diverse long-term exercise intensity effects on appetite and body weight regulation: Arcuate Neuropeptide -Y and Proopiomelanocortin gene function. Physiol Pharmacol 2023; 27 (4) : 7
URL: http://ppj.phypha.ir/article-1-1979-en.html
Abstract:   (1055 Views)

Introduction: The effectiveness of various extrinsic and intrinsic regulatory signals on food intake and body weight can be influenced by hypothalamic neuropeptide-Y (NPY) and proopiomelanocortin (POMC) neurons. While several studies emphasize the vital role of regular physical activity in effective weight management, how these molecular and cellular processes interact with physical activity remains an area in need of further exploration. Hence, this study aims to investigate the impact of various long-term physical activities intensities on the regulation of body weight and appetite.
Methods: Twenty-one Wistar rats (n=7) were randomized into three groups: 1) Control group, 2) a group engaged in regular exercise at moderate intensity for 24 weeks (24-ME, 5 days each week), and 3) a group frequently and intensively exercising over 24 weeks (24-IE, 5 days each week). Subsequently, Reverse transcription polymerase chain reaction (RT-PCR) and enzyme-linked immunosorbent assay (ELISA) methods were performed to measure gene expression of hypothalamic arcuate nucleus NPY and POMC, as well as serum levels of acyl-ghrelin and leptin.
Results: The POMC mRNA level decreased in the 24-ME group compared to the control rats. However, intensive regular exercise increased NPY expression compared to the control rats. Inversely, body weight and food intake levels were considerably higher in the 24-ME and 24-IE groups than in the control group. Different intensities of prolonged exercise seem to heighten appetite, eventually increasing body weight through distinct molecular pathways.
Conclusion: Hence, it can be concluded that prolonged intensive exercise may not be a practical approach for weight loss.

Article number: 7
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1. Ali MA, Kravitz AV. Challenges in quantifying food intake in rodents. Brain Res 2018; 1693: 188-91. [DOI:10.1016/j.brainres.2018.02.040]
2. Aronne LJ, Nelinson DS, Lillo JL. Obesity as a disease state: a new paradigm for diagnosis and treatment. Clin Cornerstone 2009a; 9: 9-25; discussion 26-9. [DOI:10.1016/S1098-3597(09)80002-1]
3. Aronne LJ, Nelinson DS, Lillo JL. Obesity as a disease state: a new paradigm for diagnosis and treatment. Clinical cornerstone 2009b; 9: 9-29. [DOI:10.1016/S1098-3597(09)80002-1]
4. Benite-Ribeiro SA, Putt DA, Santos JM. The effect of physical exercise on orexigenic and anorexigenic peptides and its role on long-term feeding control. Med Hypotheses 2016; 93: 30-3. [DOI:10.1016/j.mehy.2016.05.005]
5. Bilski J, Mańko G, Brzozowski T, Pokorski J, Nitecki J, Nitecka E, et al. Effects of exercise of different intensity on gut peptides, energy intake and appetite in young males. Ann Agric Environ Med 2013; 20.
6. Bilski J, Teległów A, Zahradnik-Bilska J, Dembiński A, Warzecha Z. Effects of exercise on appetite and food intake regulation. Medicina Sportiva 2009; 13: 82-94. [DOI:10.2478/v10036-009-0014-5]
7. Blundell JE, Stubbs R, Hughes D, Whybrow S, King N. Cross talk between physical activity and appetite control: does physical activity stimulate appetite? Proceedings of the Nutrition Society 2003; 62: 651-61. [DOI:10.1079/PNS2003286]
8. Casanova N, Finlayson G, Blundell JE, Hopkins M. Biopsychology of human appetite-understanding the excitatory and inhibitory mechanisms of homeostatic control. Curr Opin Physiol 2019; 12: 33-38. [DOI:10.1016/j.cophys.2019.06.007]
9. Chen S, Chen H, Zhou J J, Pradhan G, Sun Y, Pan H, et al. Ghrelin receptors mediate ghrelin-induced excitation of agouti-related protein/neuropeptide Y but not pro-opiomelanocortin neurons. J Neurochem 2017; 142: 512-20. [DOI:10.1111/jnc.14080]
10. De Bond JA, Smith JT. Kisspeptin and energy balance in reproduction. Reproduction 2014; 147: R53-63. [DOI:10.1530/REP-13-0509]
11. Douglas JA, King JA, McFarlane E, Baker L, Bradley C, Crouch N, et al. Appetite, appetite hormone and energy intake responses to two consecutive days of aerobic exercise in healthy young men. Appetite 2015; 92: 57-65. [DOI:10.1016/j.appet.2015.05.006]
12. Ezzati M, Lopez AD, Rodgers AA, Murray CJL. Comparative quantification of health risks : global and regional burden of disease attributable to selected major risk factors. World Health Organization 2004.
13. Farhadipour M, Depoortere I. The function of gastrointestinal hormones in obesity-implications for the regulation of energy intake. Nutrients 2021; 13. [DOI:10.3390/nu13061839]
14. Guelfi KJ, Donges CE, Duffield R. Beneficial effects of 12 weeks of aerobic compared with resistance exercise training on perceived appetite in previously sedentary overweight and obese men. Metabolism 2013; 62: 235-43. [DOI:10.1016/j.metabol.2012.08.002]
15. Hagobian TA, Sharoff CG, Stephens BR, Wade GN, Silva JE, Chipkin SR, et al. Effects of exercise on energy-regulating hormones and appetite in men and women. Am J Physiol Regul Integr Comp Physiol 2009; 296: R233-42. [DOI:10.1152/ajpregu.90671.2008]
16. Han D, Kim S, Cho B. mRNA expression on neuropeptide Y (NPY) to exercise intensity and recovery time. J Phys The Sci 2011; 23: 781-4. [DOI:10.1589/jpts.23.781]
17. Heymsfield SB, Greenberg AS, Fujioka K, Dixon RM, Kushner R, Hunt T, et al. Recombinant leptin for weight loss in obese and lean adults: a randomized, controlled, dose-escalation trial. JAMA 1999; 282: 1568-75. [DOI:10.1001/jama.282.16.1568]
18. Hill JW, Elmquist JK, Elias CF. Hypothalamic pathways linking energy balance and reproduction. Am J Physiol Endocrinol Metab 2008; 294: E827-32. [DOI:10.1152/ajpendo.00670.2007]
19. Holliday A, Blannin A. Appetite, food intake and gut hormone responses to intense aerobic exercise of different duration. J Endocrinol 2017; 235: 193-205. [DOI:10.1530/JOE-16-0570]
20. Jiaxu C, Weiyi Y. Influence of acute and chronic treadmill exercise on rat brain POMC gene expression. Med Sci Sports Exerc 2000; 32: 954-7. [DOI:10.1097/00005768-200005000-00012]
21. Khajehnasiri N, Dehkordi MB, Amini-Khoei H, Mohammadabadi MSM, Sadeghian R. Effect of exercise intensity and duration on the levels of stress hormones and hypothalamic-pituitary-gonadal axis in adult male rats: an experimental study. Hormones (Athens) 2021; 20: 483-90. [DOI:10.1007/s42000-021-00303-4]
22. Khajehnasiri N, Khazali H, Sheikhzadeh F. Various responses of male pituitary-gonadal axis to different intensities of long-term exercise: Role of expression of KNDYrelated genes. J Biosci 2018; 43: 569-574. [DOI:10.1007/s12038-018-9782-1]
23. Khajehnasiri N, Khazali H, Sheikhzadeh F, Ghowsi M. One-month of high-intensity exercise did not change the food intake and the hypothalamic arcuate nucleus proopiomelanocortin and neuropeptide Y expression levels in male Wistar rats. Endocr Regul 2019; 53: 8-13. [DOI:10.2478/enr-2019-0002]
24. Klok MD, Jakobsdottir S, Drent ML. The role of leptin and ghrelin in the regulation of food intake and body weight in humans: a review. Obes Rev 2007; 8: 21-34. [DOI:10.1111/j.1467-789X.2006.00270.x]
25. Laing BT, Do K, Matsubara T, Wert DW, Avery MJ, Langdon EM, et al. Voluntary exercise improves hypothalamic and metabolic function in obese mice. J Endocrinol 2016; 229: 109-22. [DOI:10.1530/JOE-15-0510]
26. Lauterio TJ, Davies MJ, DeAngelo M, Peyser M, Lee J. Neuropeptide Y expression and endogenous leptin concentrations in a dietary model of obesity. Obes Res 1999; 7: 498-505. [DOI:10.1002/j.1550-8528.1999.tb00439.x]
27. Owyang C, Heldsinger A. Vagal control of satiety and hormonal regulation of appetite. J Neurogastroenterol Motil 2011; 17: 338-48. [DOI:10.5056/jnm.2011.17.4.338]
28. Pomerleau M, Imbeault P, Parker T, Doucet E. Effects of exercise intensity on food intake and appetite in women. Am J Clin Nutr 2004; 80: 1230-6. [DOI:10.1093/ajcn/80.5.1230]
29. Sadeghian R, Shahidi S, Komaki A, Habibi P, Ahmadiasl N, Yousefi H, et al. Synergism effect of swimming exercise and genistein on the inflammation, oxidative stress, and VEGF expression in the retina of diabetic-ovariectomized rats. Life Sci 2021; 284: 119931. [DOI:10.1016/j.lfs.2021.119931]
30. Salehi M S, Namavar MR, Shirazi MRJ, Rahmanifar F, Tamadon A. A simple method for isolation of the anteroventral periventricular and arcuate nuclei of the rat hypothalamus. Anatomy 2012; 7. [DOI:10.2399/ana.11.212]
31. Sartin JL, Daniel JA, Whitlock BK, Wilborn RR. Selected hormonal and neurotransmitter mechanisms regulating feed intake in sheep. Animal 2010; 4: 1781-9. [DOI:10.1017/S1751731110001497]
32. Sohn JW. Network of hypothalamic neurons that control appetite. BMB Rep 2015; 48: 229-33. [DOI:10.5483/BMBRep.2015.48.4.272]
33. Stubbs R, Sepp A, Hughes D, Johnstone A, King N, Horgan G, et al. The effect of graded levels of exercise on energy intake and balance in free-living women. Int J Obes 2002; 26: 866-9. [DOI:10.1038/sj.ijo.0801874]
34. Varela L, Horvath TL. Leptin and insulin pathways in POMC and AgRP neurons that modulate energy balance and glucose homeostasis. EMBO Rep 2012; 13: 1079-86. [DOI:10.1038/embor.2012.174]
35. Wardlaw SL. Clinical review 127: Obesity as a neuroendocrine disease: lessons to be learned from proopiomelanocortin and melanocortin receptor mutations in mice and men. J Clin Endocrinol Metab 2001; 86: 1442-6. [DOI:10.1210/jcem.86.4.7388]

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