Volume 28, Issue 1 (March 2024)                   Physiol Pharmacol 2024, 28(1): 56-65 | Back to browse issues page


XML Print


Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:

Heydari Z, Sadeghi-Dinani M, Talebi A, Minaiyan M. Evaluation of anti-ulcerative effect of Eryngium billardieri extracts on experimental colitis in rats. Physiol Pharmacol 2024; 28 (1) : 8
URL: http://ppj.phypha.ir/article-1-2156-en.html
Abstract:   (668 Views)
Introduction: Eryngium billardieri has been demonstrated in previous studies to possess anti-inflammatory, antioxidant, and wound-healing properties. Colitis, an inflammatory bowel disease with unknown causes, often leads to numerous side effects associated with current medications. Therefore, this study aimed to investigate the anti-ulcerative potential of E. billardieri extracts in experimental colitis.
Methods: The hydroalcoholic extract of E. billardieri and its aqueous and ethyl acetate partitions were prepared using the maceration method, and the polyphenol content was determined for each extract. Male Wistar rats with acetic acid-induced colitis were orally administered three different doses (50, 100, 200 mg/kg) of the extract and each partition for 5 consecutive days. On the sixth day, the rats’ colons were removed and analyzed for macroscopic parameters (ulcer index), microscopic parameters (total colitis index), as well as inflammatory and oxidative stress markers, including myeloperoxidase and malondialdehyde, respectively.
Results: The total phenol content for the dry extract and aqueous and ethyl acetate partitions were 6.51, 4.15, and 8.59 mg gallic acid equivalent/g, respectively. The hydroalcoholic extract and ethyl acetate partition at all three examined doses were able to significantly alleviate most parameters related to colitis. However, the aqueous partition did not improve most of the colitis features except for the tissue level of malondialdehyde.
Conclusion: The study concludes that the total extract of E. billardieri, as well as the ethyl acetate partition, exhibited anti-colitis properties in a dose-related manner. It is suggested that the effective substances responsible for these properties are non-polar compounds that are not extracted by aqueous partitioning. Further studies are needed to identify and characterize these effective compounds.
Article number: 8
Full-Text [PDF 987 kb]   (98 Downloads)    

References
1. Akshaya K, Chitra V. A review on pathological state and herbal remedies on ulcerative colitis. Res J Pharm Technol. 2019; 12(3): 1409-17. [DOI:10.5958/0974-360X.2019.00235.X]
2. Baumgart D C, Carding S R. Inflammatory bowel disease: cause and immunobiology. The Lancet. 2007; 12;369: 1627-40. [DOI:10.1016/S0140-6736(07)60750-8]
3. Cai Z, Wang S, Li J. Treatment of inflammatory bowel disease: a comprehensive review. Front Med. 2021; 20(8): 765474. [DOI:10.3389/fmed.2021.765474]
4. Calvino C I, Martínez S G, Downie S R. The evolutionary history of Eryngium (Apiaceae, Saniculoideae): rapid radiations, long distance dispersals, and hybridizations. Mol Phylogenet Evol. 2008; 46(3): 1129-50. [DOI:10.1016/j.ympev.2007.10.021]
5. Cosnes J, Gower-Rousseau C, Seksik P, Cortot A. Epidemiology and natural history of inflammatory bowel diseases. Gastroenterology. 2011; 140(6): 1785-94. [DOI:10.1053/j.gastro.2011.01.055]
6. Daneshzadeh M S, Abbaspour H, Amjad L, Nafchi A M. An investigation on phytochemical, antioxidant and antibacterial properties of extract from Eryngium billardieri F. Delaroche. J Food Me::as char::a. 2020; 14(2): 708-15. [DOI:10.1007/s11694-019-00317-y]
7. Frankel E N, Meyer A S. The problems of using one-dimensional methods to evaluate multifunctional food and biological antioxidants. J Sci Food Agr. 2000; 80(13): 1925-41. [DOI:10.1002/1097-0010(200010)80:133.0.CO;2-4]
8. Gajendran M, Loganathan P, Jimenez G, Catinella A P, Ng N, Umapathy C, et al. A comprehensive review and update on ulcerative colitis. Dis Mon. 2019; 65(12): 100851. [DOI:10.1016/j.disamonth.2019.02.004]
9. Gholap P A, Nirmal S A, Pattan S R, Pal S C, Mandal S C. Potential of Moringa oleifera root and Citrus sinensis fruit rind extracts in the treatment of ulcerative colitis in mice. Pharm Biol. 2012; 50(10): 1297-02. [DOI:10.3109/13880209.2012.674142]
10. Handa S S, Khanuja S P, Longo G, Rakesh D U. editors. Extraction technologies for medicinal and aromatic plants. 1st ed. Trieste: ISC-UNDO publication; 2008.
11. Heidari B, Sajjadi S E, Minaiyan M. Effect of Coriandrum sativum hydroalcoholic extract and its essential oil on acetic acid- induced acute colitis in rats. Avicenna J Phytomed 2016; 6(2): 205-14.
12. Ishihara S, Aziz M M, Yuki T, Kazumori H, Kinoshita Y. Inflammatory bowel disease: review from the aspect of genetics. J Gastroenterol. 2009; 44: 1097-08. [DOI:10.1007/s00535-009-0141-8]
13. Khani S, Abdollahi M, Asadi Z, Nazeri M, Nasiri M A, Yusefi H, et al. Hypoglycemic, hepatoprotective, and hypolipidemic effects of hydroalcoholic extract of Erynium billardieri root on nicotinamide/streptozotocin-induced type II diabetic rats. Res Pharm Sci. 2021; 16(2): 193. [DOI:10.4103/1735-5362.310526]
14. Khoramian L, Sajjadi SE, Miniaiyan M. Anti-inflammatory effect of Adiantum capillus-veneris hydroalcoholic and aqueous extracts on acetic acid-induced colitis in rats. Avicenn J Phytomed 2020; 10(5): 492-03.
15. Kumar V S, Rajmane A R, Adil M, Kandhare A D, Ghosh P, Bodhankar S L. Naringin ameliorates acetic acid induced colitis through modulation of endogenous oxido-nitrosative balance and DNA damage in rats. J Biomed Res. 2014; 28(2): 132. [DOI:10.7555/JBR.27.20120082]
16. MacPherson B, Pfeiffer C. Experimental production of diffuse colitis in rats. Digestion. 1978; 17(2): 135-50. [DOI:10.1159/000198104]
17. Maisuthisakul P, Suttajit M, Pongsawatmanit R. Assessment of phenolic content and free radical-scavenging capacity of some Thai indigenous plants. Food Chem. 2007; 100(4): 1409-18. [DOI:10.1016/j.foodchem.2005.11.032]
18. Minaiyan M, Ghannadi A, Mahzouni P, Nabi-Meibodi M. Anti-ulcerogenic effect of ginger (rhizome of Zingiber officinale Roscoe) hydroalcoholic extract on acetic acid-induced acute colitis in rats. Res Pharm Sci. 2008;3(2):79-86.
19. Minaiyan M. Sajjadi SE, Naderi N, Taheri D. Anti-inflammatory effect of Kelussia odoratissima Mozaff. hydroalcoholic extract on acetic acid- induced acute colitis in rats. J Reports Pharm Sci 2014; 3(1): 28-35. [DOI:10.4103/2322-1232.222545]
20. Motavallian-Naeini A, Minaiyan M, Rabbani M, Mahzuni P. Anti-inflammatory effect of ondansetron through 5-HT3 receptors on TNBS-induced colitis in rat. EXCLI J. 2012; 11:30.
21. Ng S C, Shi H Y, Hamidi N, Underwood F E, Tang W, Benchimol E I, et al. Worldwide incidence and prevalence of inflammatory bowel disease in the 21st century: a systematic review of population-based studies. Lancet. 2017; 390: 2769-78. [DOI:10.1016/S0140-6736(17)32448-0]
22. Niknami E, Sajjadi S E, Talebi A, Minaiyan M. Protective effect of Vitis vinifera (Black Grape) seed extract and oil on acetic acid-induced colitis in rats. Int J Prev Med 2020; 362: 19.
23. Perler B K, Ungaro R, Baird G, Mallette M, Bright R, Shah S, et al. Presenting symptoms in inflammatory bowel disease: descriptive analysis of a community-based inception cohort. BMC Gastroenterol. 2019;19(1), 1-8. [DOI:10.1186/s12876-019-0963-7]
24. Roshanravan N, Asgharian P, Dariushnejad H, Alamdari N M, Mansoori B, Mohammadi A, et al. Eryngium billardieri induces apoptosis via bax gene expression in pancreatic cancer cells. Adv Pharm Bull. 2018; 8(4): 667. [DOI:10.15171/apb.2018.075]
25. Sefidkon F, Dabiri M, Alamshahi A. Chemical composition of the essential oil of eryngium billardieri F. delaroche from Iran. J Essen Oil Res. 2004; 16(1): 42-3. [DOI:10.1080/10412905.2004.9698648]
26. Siddhuraju P, Becker K. Antioxidant properties of various solvent extracts of total phenolic constituents from three different agroclimatic origins of drumstick tree (Moringa oleifera Lam.) leaves. J Agr Food Chem. 2003; 51(8): 2144-55. [DOI:10.1021/jf020444+]
27. Tahan G, Aytac E, Aytekin H, Gunduz F, Dogusoy G, Aydin S, et al. Vitamin E has a dual effect of anti-inflammatory and antioxidant activities in acetic acid-induced ulcerative colitis in rats. Can J Surg. 2011; 54(5): 333. [DOI:10.1503/cjs.013610]
28. Thiviya P, Gamage A, Piumali D, Merah O, Madhujith T. Apiaceae as an important source of antioxidants and their applications. Cosmetics. 2021; 8(4): 111. [DOI:10.3390/cosmetics8040111]
29. Ungaro R, Colombel J F, Lissoos T, Peyrin-Biroulet L. A treat-to-target update in ulcerative colitis: a systematic review. Am J Gastroenterol. 2019; 114(6): 874. [DOI:10.14309/ajg.0000000000000183]
30. Wang P, Su Z, Yuan W, Deng G, Li S. Phytochemical constituents and pharmacological activities of Eryngium billardieri (Apiaceae). 2012; 3(1): 99-120. [DOI:10.2174/2210290601203010099]
31. Yesilada E, Tanaka S h, Tabata M, Sezik E. The anti-inflammatory activity of the fractions from Eryngium billardieri in mice. Phytother Res. 1989; 3(1): 38-40. [DOI:10.1002/ptr.2650030111]
32. Zarei A, Ashtiyani S C, Hamidizadeh S, Rezaei A, Ramezani M, Aalizadeh A. The study of the effects hydro-alcoholic extract of Eryngium billardieri on lipid profiles levels and liver and renal functions tests in hypercholesterolemic rats. J Chem Pharm Res. 2015; 7(2): 200-6.
33. Zargari A. Pharmaceutical plants (persian). 8th ed. Tehran: Tehran University Press; 2015. 212-4.

Rights and permissions
Creative Commons License This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.