Volume 29, Issue 2 (June 2025)                   Physiol Pharmacol 2025, 29(2): 155-170 | Back to browse issues page

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Zhaeentan S, Amidi F, Moini A, Mohammadi S D, Sobhani A, Nataj Majd M et al . Identification of endometriosis molecular regulatory axes through bioinformatics analysis: insights into lncRNAs, miRNAs, and mRNAs. Physiol Pharmacol 2025; 29 (2) :155-170
URL: http://ppj.phypha.ir/article-1-2353-en.html
Identification of endometriosis molecular regulatory axes through bioinformatics analysis: insights into lncRNAs, miRNAs, and mRNAs
Shahrzad Zhaeentan , Fardin Amidi , Ashraf Moini , Seyed Danial Mohammadi , Aligholi Sobhani , Masoomeh Nataj Majd , Mahshad Khodarahmian
Abstract:   (980 Views)

Introduction: Endometriosis (EMS) is a highly prevalent gynecological disorder with substantial health consequences, affecting as many as 10% of women in their reproductive years. Although EMS is widespread, its intricate origin and pathophysiology are not well understood. This study investigated the molecular characteristics of EMS by examining the expression patterns of long noncoding RNAs (lncRNAs), microRNAs (miRNAs), and genes linked to the condition.
Methods: The study used publicly accessible datasets, namely RNA-seq data for long non-coding RNAs (GSE105764), miRNA expression data (GSE105765), and gene expression data (GSE12768), to compare samples of ectopic and eutopic endometrial samples. The study conducted differential expression analysis, pathway enrichment analyses, protein-protein interaction network creation, and hub gene identification to uncover the molecular markers linked to EMS. 
Results: The investigation identified substantial dysregulation of lncRNAs, miRNAs, and genes in endometriotic tissues compared with the control eutopic endometrium. Hub genes, including CDH1, ESR1, GATA4, PGR, FOXA2, EPCAM, APOA1, BDNF, FGFR2, and PAX2, dominate the molecular landscape. miR-6500-3p has been identified as a pivotal regulator that influences the expression of seven essential genes. Pathway enrichment analysis identified biological processes, including embryonic organ morphogenesis, immunological response, and medication metabolism. Exploring lncRNA-mediated regulatory axes, particularly H19 and MIR600HG, suggests complex networks that may clarify the molecular underpinnings of EMS.
Conclusion: This thorough investigation offers significant insight into the molecular pathways underlying EMS. Hub genes and regulatory miRNAs, including miR-6500-3p, have been identified as prospective targets for therapeutic interventions. Pathway enrichment studies provide a more comprehensive understanding of the biological processes implicated in EMS, indicating new possibilities for therapeutic interventions.

Keywords: Endometriosis, lncRNAs, miRNAs, Hub Genes, Bioinformatics
Full-Text [PDF 2369 kb]   (50 Downloads)    
Type of Manuscript: Clinical research article | Subject: Clinical Physiology/Pharmacology
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