Volume 29, Issue 4 (December 2025)                   Physiol Pharmacol 2025, 29(4): 386-397 | Back to browse issues page

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Sazegar G, Rajabzadeh A A, Paseban M, Khoshdel- Sarkarizi H. Co-administration of hydro-alcoholic Teucrium polium l. extract and glibenclamide on pancreatic islets in streptozotocin-induced diabetic rats- a stereological analysis. Physiol Pharmacol 2025; 29 (4) :386-397
URL: http://ppj.phypha.ir/article-1-2421-en.html
Co-administration of hydro-alcoholic Teucrium polium l. extract and glibenclamide on pancreatic islets in streptozotocin-induced diabetic rats- a stereological analysis
Ghasem Sazegar , Ali Akbar Rajabzadeh , Maryam Paseban , Hoda Khoshdel- Sarkarizi
Abstract:   (1761 Views)

Introduction: As herbal drugs have the potential to influence the therapeutic outcomes of pharmaceutical drugs, we investigated the effects of co-administration of Teucrium polium L. extract (TP) and Glibenclamide (Glib) on pancreatic islets in diabetic rats.
Methods: Male Wistar rats were separated into six distinct groups (n=8). The control and sham control (normal saline), while four diabetic rats received different treatments (normal saline, Glib (5mg/kg), TP (200mg/kg), or co-administration of TP and Glib), via gavage, for 6 weeks. Induction of diabetes was performed with Streptozotocin injection, intraperitoneally at a dose of 55 mg/kg. The animals were anesthetized, and their dissected pancreases were fixed in 10% Formalin. Stereological assessments were done to determine pancreas volume, islet volume, volume density of islets relative to the pancreas, and the number of beta and apoptotic cells within the islets. Ultimately, the data analysis was conducted using SPSS and ANOVA.
Results: Histological examinations revealed that the administration of TP, Glib, and their co administration did not non-significantly increase the islet volume and volume density of islets relative to the pancreas in diabetic rats. However, treatment with these drugs led to an increase in the number of beta-cells and a decrease in the number of apoptotic cells within the islet. Notably, co-administration of these drugs did not yield significant differences compared to individual treatments.
Conclusion: This study demonstrates that TP and Glib have similar impacts in streptozotocin induced diabetic rats. Co-administration of the two agents did not lead to a statistically significant difference compared with either treatment alone.

Keywords: Diabetes, Teucrium polium, Glibenclamide, Pancreas, Stereology
Full-Text [PDF 4856 kb]   (33 Downloads)    
Type of Manuscript: Experimental research article | Subject: Clinical Physiology/Pharmacology
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