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Complex of zinc (II) methylthiosemicarbazone Induces Cytotoxicity, Antiproliferative and Apoptosis effects in Human K562 leukemia
Simin Namvar aghdash , Majid Mahdavi , Golsa Foroughi
Abstract:   (7 Views) 
Introduction: Methylthiosemicarbazones (MTSCs) is a compound with different biological properties. Some studies demonstrated its metal complexes have potential cytotoxic effects on numerous cancers. In this study the effects of Zn2+-methylthiosemicarbazone complex (Zn-MTC) on human chronic myelogenous leukemia K562 cell line were evaluated.
Methods: In this experimental study, K562 cells were treated with different doses of Zinc (II) methylthiosemicarbazone (Zn-MTC) for 24, 48 and 72 h. We studies whether the compound could induce cytotoxicity and apoptosis in K562 cells using MTT assay, fluorescence microscopy, and flow cytometry.
Results: Our results indicated that Zn-MTC time and dose dependently inhibited the growth of K562 cells and induced apoptosis. The IC50 value of the complex was 100 µM after 72h incubation. Morphological changes and the Sub-G1 cell cycle confirmed apoptosis induction in K562 cells that were treated with the IC50 value of the compound.
Conclusion: Considering the prevention of proliferation and induction of apoptosis effects of Zn-MTC, this compound can be suggested for further pharmaceutical evaluation for treatment of CML patients in the future.
 
Keywords: Methylthiosemicarbazone, Apoptosis, Cytotoxicity, cell cycle, K562 cell line.
     
Type of Manuscript: Short communication | Subject: Others
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