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Abstract: (29 Views)
Background: Medicinal plants perform a prominent function in improving sustainable healthcare system and benefits because of enriching bioactive components in pharmacology industry. The aim for present research is to explore potential medicinal attributes of Teucrium polium L. essential oil (TEO), focusing on antioxidant, anticancer and antimicrobial attributes.
Methods: Initially, TEO isolated by distillation method and existence substances such as spathulenol (12.98 %), beta-pinene (10.07 %) and germacrene B (9.21 %) identified using gas chromatography. Functional groups detected in TEO, which confirmed through Fourier transform infrared spectroscopy.
Results: In addition, total phenol and flavonoids were reported 198.1 ± 4.19 mg gallic acid equivalent (GAE)/g dry weight (DW) and 56.23 ± 9 mg rutin (R)E/g DW, respectively. The promising antioxidant function of TEO was proved using achieved IC50 from 2,2-diphenyl-1-picrylhydrazyl (94.35 ±3.2 μg/mL), 2,2'-azino-bis-3-ethylbenzothiazoline-6-sulfonic acid (83.3 ± 5.1 μg/mL) and ferric ions (Fe3+) reducing antioxidant power (2.8 ± 0.02 μg ascorbic acid equivalent (AAE)/g). Additionally, an acceptable cytotoxicity detected by TEO according to obtained results. The lowest (1.8 mm) and highest (13.8 mm) inhibition zones for TEO were observed against Escherichia coli (E. coli) at a concentration of 20 mg/L and Staphylococcus aureus at 80 mg/L level in disk diffusion agar, respectively. The structure of E. coli depicted using a scanning electron microscopy evaluation after treating by TEO, which indicated several effects on membrane and denaturalization for bacterial cell.
Conclusion: Overall, significant antimicrobial, antioxidant and anticancer functions of TEO suggested a candidate for further research in pharmacology industry and novel therapeutic agents.
Methods: Initially, TEO isolated by distillation method and existence substances such as spathulenol (12.98 %), beta-pinene (10.07 %) and germacrene B (9.21 %) identified using gas chromatography. Functional groups detected in TEO, which confirmed through Fourier transform infrared spectroscopy.
Results: In addition, total phenol and flavonoids were reported 198.1 ± 4.19 mg gallic acid equivalent (GAE)/g dry weight (DW) and 56.23 ± 9 mg rutin (R)E/g DW, respectively. The promising antioxidant function of TEO was proved using achieved IC50 from 2,2-diphenyl-1-picrylhydrazyl (94.35 ±3.2 μg/mL), 2,2'-azino-bis-3-ethylbenzothiazoline-6-sulfonic acid (83.3 ± 5.1 μg/mL) and ferric ions (Fe3+) reducing antioxidant power (2.8 ± 0.02 μg ascorbic acid equivalent (AAE)/g). Additionally, an acceptable cytotoxicity detected by TEO according to obtained results. The lowest (1.8 mm) and highest (13.8 mm) inhibition zones for TEO were observed against Escherichia coli (E. coli) at a concentration of 20 mg/L and Staphylococcus aureus at 80 mg/L level in disk diffusion agar, respectively. The structure of E. coli depicted using a scanning electron microscopy evaluation after treating by TEO, which indicated several effects on membrane and denaturalization for bacterial cell.
Conclusion: Overall, significant antimicrobial, antioxidant and anticancer functions of TEO suggested a candidate for further research in pharmacology industry and novel therapeutic agents.
Type of Manuscript: Experimental research article |
Subject:
Cell, Stem Cell and Cancer
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