Volume 17, Issue 4 (Winter 2014)
Physiol Pharmacol 2014, 17(4): 370-378 |
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Jahanara M, Tamadon A, Jafarzadeh Shirazi M R, Rahmanifar F, Sabet Sarvestani F, Tanideh N, et al . Long term malnutrition and mRNAs expression of RFRP-3 and KiSS-1 in hypothalamus of female ovariectomized rats. Physiol Pharmacol 2014; 17 (4) :370-378
URL: http://ppj.phypha.ir/article-1-917-en.html
URL: http://ppj.phypha.ir/article-1-917-en.html
Maliheh Jahanara 
, Amin Tamadon , Mohammad Reza Jafarzadeh Shirazi , Farhad Rahmanifar , Fatemeh Sabet Sarvestani , Nader Tanideh , Ali Moghadam , Ali Niazi
, Amin Tamadon , Mohammad Reza Jafarzadeh Shirazi , Farhad Rahmanifar , Fatemeh Sabet Sarvestani , Nader Tanideh , Ali Moghadam , Ali Niazi
Abstract: (9005 Views)
Introduction: Arginine-phenylalanine-amide-related peptide-3 (RFRP-3) is an inhibitor of gonadotropin releasing
hormone (GnRH) secretion and an appetizer. Kisspeptin is a stimulator of GnRH and a regulator of energy metabolism.
The objectives of the present study were to compare the effect of long term malnutrition on expression of RFRP-3
mRNA in dorsomedial hypothalamic nucleus (DMH) and KiSS-1 mRNA in arcuate nucleus (ARC) of rat
hypothalamus.
Methods: Sixteen female ovariectomized rats of the Sprague-Dawley strain were randomly allotted into three
groups. After 2 weeks recovery period, one group was fed with perfect diet (rat standard feed) and the other group was
fed with the half dietary of perfect diet for 14 days. The control group (n=4) were sacrificed 2 weeks after surgery (day
0). Relative expression of RFRP-3 and KiSS-1 mRNAs (compared to the control group) were measured using real-time
PCR method in DMH and ARC of hypothalamus, respectively.
Results: Mean and SE of relative expression of RFRP-3 mRNA in DMH in the half dietary rats was higher than that
of the perfect diet ones (P=0.01). Relative expression of KiSS-1 mRNA in ARC was not different between two groups
of rats (P=0.1).
Conclusion: Long term malnutrition (2 weeks) increased RFRP-3 mRNA expression in DMH of rats hypothalamus,
but had no effect on KiSS-1 mRNA expression in the ARC nucleus of ovariectomized female rats.
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