Volume 30, Issue 1 (March 2026)                   Physiol Pharmacol 2026, 30(1): 22-37 | Back to browse issues page

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Khoshsirat S, bazgir N, mosleh H, Raissi S, amanzadeh jajin E, Niknazar S. A systematic review and bioinformatics analysis on DNA methylation status in age-related hearing loss. Physiol Pharmacol 2026; 30 (1) :22-37
URL: http://ppj.phypha.ir/article-1-2460-en.html
A systematic review and bioinformatics analysis on DNA methylation status in age-related hearing loss
Shahrokh Khoshsirat , Narges Bazgir , Haideh Mosleh , Simin Raissi , Elanaz Amanzadeh jajin , Somayeh Niknazar
Abstract:   (1137 Views)
Many individuals experience age-related hearing loss (ARHL), yet it frequently goes unnoticed. Its prevalence increases two-fold with every ten years of age. Epigenetics is linked to heritable modifications in gene activity that are not caused by alterations in DNA sequence. In this review, our goal is to assess the variety of DNA methylation in individuals with ARHL. Methods: We conducted a systematic review of studies that report changes in DNA methylation among patients with presbycusis. The study used the PECO framework to establish the eligibility criteria for patients with ARHL. A search was performed on Embase, PubMed, Scopus, Google Scholar, and ScienceDirect. Furthermore, a bioinformatics analysis was performed. Results: A total of seven studies were reviewed. Eighteen genes including P2RX2, KCNQ5, SOCS3, ERBB3, TCF25, POLE, ESPN, TNFRSF25, FGFR1, CDH23, SLC26A4, MEF2D, LCK, ACP6, ALG10, DUSPK4, C21orf58 and GBX2 were differentially methylated. To contextualize these in ARHL pathways, we integrated them with 136 ARHLassociated genes for PPI network analysis, identifying nine hub genes. Then we performed the overlap analysis using R to identify intersections between 18 methylated genes and the hub genes. After conducting enrichment analysis for hub genes, differentially methylated genes, and intersected genes, it was revealed that ERBB3, P2RX2, and FGFR1 were involved in the calcium signaling pathway. Besides, the overlap of methylated genes with ARHL hubs identifies SLC26A4, ERBB3, and CDH23 as key candidates. Conclusion: This review demonstrated that DNA methylation could cause the development of ARHL. Changes in gene methylation can disrupt the calcium signaling pathway, potentially leading to presbycusis.
Keywords: DNA Methylation, Age-Related Hearing Loss, Systematic Review, Bioinformatics Analysis
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Type of Manuscript: Review | Subject: Clinical Physiology/Pharmacology
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